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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1628 [new locus tag: SACOL_RS08300 ]
  • pan locus tag?: SAUPAN004153000
  • symbol: SACOL1628
  • pan gene symbol?: phoH
  • synonym:
  • product: PhoH family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1628 [new locus tag: SACOL_RS08300 ]
  • symbol: SACOL1628
  • product: PhoH family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1657881..1658801
  • length: 921
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAACCAATCTCAAGCTTTAATTGGAAATAATGATGAACATTTAAAAGCAATTGAAGAG
    AGTTTCGATGTTGTCATCCATGCAAGAGGACAAGAAGTTGCCGTTAAAGGTACAAAAATA
    GAAAACGTAGAAAAAGCGGAATCAGTATTAATCAATTTGCTGAAGGTTATTGATTTAGGT
    AATAATATTACAATTAAAGATGTTGAAGCAGCTATTAAAATGGCGCATAATAACACAATT
    CAACATCTGTTAGATTTATATGATGAAGAGATAACTAAAGATGCATTTGGTAAGACGATT
    CGTGCGAAAACGATGGGGCAACGTATATATGTTAATGCCATGAAAAATAATGATTTAGTA
    TTTGGTATAGGTCCTGCTGGTACAGGTAAGACATTCTTAGCTGTAGTTTATGCAGCAAAG
    CAACTCCGTAAAGGTGCTGTTAAACGTATTGTATTAACAAGACCTGCTGTTGAAGCAGGA
    GAGTCACTTGGATTTTTACCAGGAGATTTGAAAGAAAAGGTAGATCCATATTTAAGACCT
    TTATATGATGGTCTATATACTGTTCTTGGGCGTGAACAAACAGAGCGATTTATTGAAAGA
    GGCATTATCGAAATAGCGCCACTTGCATATATGCGCGGACGAACATTAGAAGATGCATTT
    GTAATTCTTGATGAGGCGCAGAATACGACACATGCGCAAATGAAAATGTTTTTAACAAGA
    CTAGGTTTTGGCTCAAAAATGGTAGTTACTGGTGACCAAACTCAAATCGATTTACCTAAA
    GGTGTTAAAAGTGGACTTAAGGAAGCGGTCAGTAGGTTACACAACGTTAAAGGTATAAGT
    ATATTGAAATTAGATCAGAGCGATGTAGTCAGACATCCATTGGTAAGTAAGATCATTGAA
    CATTATGAAGGAGAGAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    921

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1628 [new locus tag: SACOL_RS08300 ]
  • symbol: SACOL1628
  • description: PhoH family protein
  • length: 306
  • theoretical pI: 7.95346
  • theoretical MW: 33930
  • GRAVY: -0.180065

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair putative DNA helicase (TIGR00376; EC 3.6.4.12; HMM-score: 16.9)
  • TheSEED  :
    • PhoH-like protein, PhoL
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory Phosphate metabolism  Phosphate starvation-inducible protein PhoH, predicted ATPase
  • PFAM:
    P-loop_NTPase (CL0023) PhoH; PhoH-like protein (PF02562; HMM-score: 314.8)
    and 10 more
    AAA_30; AAA domain (PF13604; HMM-score: 35.1)
    AAA_19; AAA domain (PF13245; HMM-score: 24.9)
    AAA_22; AAA domain (PF13401; HMM-score: 19)
    KH (CL0007) KH_1; KH domain (PF00013; HMM-score: 17.8)
    P-loop_NTPase (CL0023) IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 16.8)
    UvrD-helicase; UvrD/REP helicase N-terminal domain (PF00580; HMM-score: 13.8)
    DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 13.6)
    Zot; Zonular occludens toxin (Zot) (PF05707; HMM-score: 13.6)
    no clan defined Vps16_C; Vps16, C-terminal region (PF04840; HMM-score: 12.8)
    P-loop_NTPase (CL0023) AAA_25; AAA domain (PF13481; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001752
    • TAT(Tat/SPI): 0.000169
    • LIPO(Sec/SPII): 0.000315
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAVKGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITKDAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIVLTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLAYMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLKEAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 176 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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