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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1623 [new locus tag: SACOL_RS08275 ]
  • pan locus tag?: SAUPAN004147000
  • symbol: SACOL1623
  • pan gene symbol?: recO
  • synonym:
  • product: RecO family DNA repair protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1623 [new locus tag: SACOL_RS08275 ]
  • symbol: SACOL1623
  • product: RecO family DNA repair protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1654977..1655729
  • length: 753
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    TTGTTAATGCGCCAAAAAGGGATTATCATCAAAGCAGTTGATTATGGTGAATCTGATAAA
    ATTATCACGATTTTAAATGAGCATGGTGCAAAAGTACCACTTATGGCAAGGCGTGCTAAA
    AAAGTTAAGACGGGTTTACAAGCGCAAACGCAATTGTTTGTTTATGGTTTGTTTATTTAC
    AATCAGTGGCGAGGTATGGGAACGTTAAATTCTGTAGATGTTATTAGTCAACATTATAAA
    TTACAAATGGACCTTTACGTAAGCAGTTATGCCGCTCTGGCAGCTGAAACTATTGAGCGC
    TCAATGGATGAAGGTGACATTGCACCATATAACTATCAATTATTACAATTTGTTCTTGAA
    AAAATAGAATCAGGTACATCTGCACAGTTAATGTCAGTCGTAGTTATGTTAAAGTGCATG
    AAACGATTTGGTTTTACTGCATCATTTAATCGCTGTGCTGTGAGTGGTAATGACACACAA
    GCAGATTTAATAGGTTATAGTTTTAAGTTTGACGGTGCGATTTCAAGGCAAGAGGCTTCT
    AAAGATGTACATGCAGTTATATTATCGAATAAAACACTATATTTATTAGATGTATTACAA
    AAATTACCGATAGATAAAATGAATTCATTGAATATCCATCAAGAAATTATTGATGAAATG
    TCAGATATCATTTTAATGTTATATCGTGAATATGCAGGTATGTTTTTTAAAAGTCAGAAA
    CTAATCAACCAATTAAAAAGATTGGAACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    753

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1623 [new locus tag: SACOL_RS08275 ]
  • symbol: SACOL1623
  • description: RecO family DNA repair protein
  • length: 250
  • theoretical pI: 8.89118
  • theoretical MW: 28450
  • GRAVY: -0.044

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA repair protein RecO (TIGR00613; HMM-score: 259.7)
  • TheSEED  :
    • DNA recombination and repair protein RecO
    DNA Metabolism DNA repair DNA repair, bacterial RecFOR pathway  DNA recombination and repair protein RecO
  • PFAM:
    Zn_Beta_Ribbon (CL0167) RecO_C; Recombination protein O C terminal (PF02565; HMM-score: 90.4)
    and 2 more
    OB (CL0021) RecO_N; Recombination protein O N terminal (PF11967; HMM-score: 59.8)
    RecO_N_2; RecO N terminal (PF13114; HMM-score: 25)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003247
    • TAT(Tat/SPI): 0.00085
    • LIPO(Sec/SPII): 0.000987
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLMRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGLQAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYAALAAETIERSMDEGDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQADLIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEMSDIILMLYREYAGMFFKSQKLINQLKRLEQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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