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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1623 [new locus tag: SACOL_RS08275 ]
- pan locus tag?: SAUPAN004147000
- symbol: SACOL1623
- pan gene symbol?: recO
- synonym:
- product: RecO family DNA repair protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1623 [new locus tag: SACOL_RS08275 ]
- symbol: SACOL1623
- product: RecO family DNA repair protein
- replicon: chromosome
- strand: -
- coordinates: 1654977..1655729
- length: 753
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3238298 NCBI
- RefSeq: YP_186463 NCBI
- BioCyc: see SACOL_RS08275
- MicrobesOnline: 913072 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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721TTGTTAATGCGCCAAAAAGGGATTATCATCAAAGCAGTTGATTATGGTGAATCTGATAAA
ATTATCACGATTTTAAATGAGCATGGTGCAAAAGTACCACTTATGGCAAGGCGTGCTAAA
AAAGTTAAGACGGGTTTACAAGCGCAAACGCAATTGTTTGTTTATGGTTTGTTTATTTAC
AATCAGTGGCGAGGTATGGGAACGTTAAATTCTGTAGATGTTATTAGTCAACATTATAAA
TTACAAATGGACCTTTACGTAAGCAGTTATGCCGCTCTGGCAGCTGAAACTATTGAGCGC
TCAATGGATGAAGGTGACATTGCACCATATAACTATCAATTATTACAATTTGTTCTTGAA
AAAATAGAATCAGGTACATCTGCACAGTTAATGTCAGTCGTAGTTATGTTAAAGTGCATG
AAACGATTTGGTTTTACTGCATCATTTAATCGCTGTGCTGTGAGTGGTAATGACACACAA
GCAGATTTAATAGGTTATAGTTTTAAGTTTGACGGTGCGATTTCAAGGCAAGAGGCTTCT
AAAGATGTACATGCAGTTATATTATCGAATAAAACACTATATTTATTAGATGTATTACAA
AAATTACCGATAGATAAAATGAATTCATTGAATATCCATCAAGAAATTATTGATGAAATG
TCAGATATCATTTTAATGTTATATCGTGAATATGCAGGTATGTTTTTTAAAAGTCAGAAA
CTAATCAACCAATTAAAAAGATTGGAACAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1623 [new locus tag: SACOL_RS08275 ]
- symbol: SACOL1623
- description: RecO family DNA repair protein
- length: 250
- theoretical pI: 8.89118
- theoretical MW: 28450
- GRAVY: -0.044
⊟Function[edit | edit source]
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair DNA repair protein RecO (TIGR00613; HMM-score: 259.7)
- TheSEED :
- DNA recombination and repair protein RecO
- PFAM: Zn_Beta_Ribbon (CL0167) RecO_C; Recombination protein O C terminal (PF02565; HMM-score: 90.4)and 2 moreOB (CL0021) RecO_N; Recombination protein O N terminal (PF11967; HMM-score: 59.8)RecO_N_2; RecO N terminal (PF13114; HMM-score: 25)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003247
- TAT(Tat/SPI): 0.00085
- LIPO(Sec/SPII): 0.000987
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MLMRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGLQAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYAALAAETIERSMDEGDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQADLIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEMSDIILMLYREYAGMFFKSQKLINQLKRLEQ
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)