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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1529 [new locus tag: SAUSA300_RS08340 ]
  • pan locus tag?: SAUPAN004151000
  • symbol: dgkA
  • pan gene symbol?: dgkA
  • synonym:
  • product: diacylglycerol kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1529 [new locus tag: SAUSA300_RS08340 ]
  • symbol: dgkA
  • product: diacylglycerol kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1679903..1680247
  • length: 345
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    ATGAAAAGGTTTAAATATGCACTTGATGGGCTGAAAATCTTAATTCAAAAAGACTATAAA
    TTTCTTTTACATGTGTTTGCAATGATTGTTGCTATTGTCTTTGGTCTCGTACTAAATATT
    AATCGGATTGAGTGGATATTTATACTCATTGCTATTGCATTAGTTCTCACTGTTGAAGCT
    TTAAACACTGCTATTGAATATGTTGTCGATTTAGTGACCGTTGAATATCATGATTTAGCT
    AAATACGCTAAAGATATTGCGGCTTTTAGTGTACTTATAGTTTCAATATTAGCATTTATT
    ATAGGTTTAATAGTATTTTTACCACATTTTATAGCGTTATTTTAG
    60
    120
    180
    240
    300
    345

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1529 [new locus tag: SAUSA300_RS08340 ]
  • symbol: DgkA
  • description: diacylglycerol kinase
  • length: 114
  • theoretical pI: 7.76368
  • theoretical MW: 12969.7
  • GRAVY: 1.35

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.107?  ExPASy
    Diacylglycerol kinase (ATP) ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate
  • TIGRFAM:
    cxxc_20_cxxc protein (TIGR04104; HMM-score: 5.3)
  • TheSEED  :
    • Undecaprenol kinase (EC 2.7.1.66)
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Diacylglycerol kinase (EC 2.7.1.107)
  • PFAM:
    no clan defined DAGK_prokar; Prokaryotic diacylglycerol kinase (PF01219; HMM-score: 112)
    and 2 more
    IT (CL0182) CitMHS; Citrate transporter (PF03600; HMM-score: 9.6)
    no clan defined SPC12; Microsomal signal peptidase 12 kDa subunit (SPC12) (PF06645; HMM-score: 8.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005147
    • TAT(Tat/SPI): 0.000083
    • LIPO(Sec/SPII): 0.001165
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIEWIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIGLIVFLPHFIALF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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