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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1653 [new locus tag: SAUSA300_RS09020 ]
  • pan locus tag?: SAUPAN004358000
  • symbol: SAUSA300_1653
  • pan gene symbol?: —
  • synonym:
  • product: metal-dependent hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1653 [new locus tag: SAUSA300_RS09020 ]
  • symbol: SAUSA300_1653
  • product: metal-dependent hydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 1818095..1818784
  • length: 690
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAGTTATCATTTCATGGTCAATCAACGATTTATTTAGAAGGGAACAACAAAAAGGTT
    ATCGTAGATCCGTTTATTTCAAATAACCCTAAGTGTGATTTGAATATTGAAACAGTGCAG
    GTAGACTATATCGTTTTAACGCACGGTCATTTTGATCATTTTGGTGATGTTGTGGAACTA
    GCAAAGAAAACAGGAGCAACAGTAATTGGAAGTGCAGAAATGGCTGACTATCTTTCTTCA
    TATCACGGTGTTGAAAATGTTCATGGTATGAATATTGGTGGTAAAGCAAACTTTGATTTC
    GGAAGCGTTAAATTTGTTCAAGCATTTCATAGTTCAAGTTTTACACATGAAAATGGTATT
    CCAGTTTATTTAGGTATGCCAATGGGTATCGTGTTTGAAGTTGAAGGAAAAACAATTTAT
    CATACTGGTGATACAGGTCTGTTTAGTGACATGTCACTTATTGCGAAACGTCATCCAGTT
    GATGTATGTTTTGTACCAATTGGAGATAATTTTACGATGGGTATTGATGATGCAAGTTAT
    GCTATTAATGAATTTATTAAACCAAAAATTTCTGTACCGATTCATTATGATACATTCCCA
    TTAATCGAACAAGATCCACAACAATTTAAAGATGCTGTTAATGTAGGGGACGTGCAAATT
    TTAAAACCAGGTGAATCTGTTCAGTTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    690

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1653 [new locus tag: SAUSA300_RS09020 ]
  • symbol: SAUSA300_1653
  • description: metal-dependent hydrolase
  • length: 229
  • theoretical pI: 5.03066
  • theoretical MW: 25250.4
  • GRAVY: -0.0462882

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Detoxification hydroxyacylglutathione hydrolase (TIGR03413; EC 3.1.2.6; HMM-score: 30.7)
    and 4 more
    arCOG00543 universal archaeal KH-domain/beta-lactamase-domain protein (TIGR03675; HMM-score: 14.6)
    Genetic information processing Transcription Degradation of RNA beta-CASP ribonuclease, RNase J family (TIGR00649; EC 3.1.-.-; HMM-score: 13.9)
    Unknown function Enzymes of unknown specificity quinoprotein relay system zinc metallohydrolase 2 (TIGR04559; EC 3.-.-.-; HMM-score: 13.6)
    Genetic information processing Transcription RNA processing ribonuclease Z (TIGR02651; EC 3.1.26.11; HMM-score: 11.2)
  • TheSEED  :
    • FIG002379: metal-dependent hydrolase
  • PFAM:
    Metallo-HOrase (CL0381) Lactamase_B_3; Beta-lactamase superfamily domain (PF13483; HMM-score: 82.1)
    Lactamase_B_2; Beta-lactamase superfamily domain (PF12706; HMM-score: 73.1)
    and 1 more
    Lactamase_B; Metallo-beta-lactamase superfamily (PF00753; HMM-score: 62.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010219
    • TAT(Tat/SPI): 0.00011
    • LIPO(Sec/SPII): 0.000935
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYIVLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGSVKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPVDVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDVQILKPGESVQF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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