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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1685 [new locus tag: SAUSA300_RS09210 ]
  • pan locus tag?: SAUPAN004406000
  • symbol: SAUSA300_1685
  • pan gene symbol?: facZ
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1685 [new locus tag: SAUSA300_RS09210 ]
  • symbol: SAUSA300_1685
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1857697..1858188
  • length: 492
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGATTGGATTTTACCAATTGCTGGAATTATCGCTGCGATTGCATTCTTAATTTTATGT
    ATCGGTATCGTAGCTGTATTAAATTCTGTTAAGAAAAACTTAGATTATGTTGCAAAAACA
    CTTGACGGTGTAGAAGGTCAAGTTCAAGGTATTACTCGTGAAACAACAGATTTACTTCAT
    AAAGTAAACCGTTTAACTGAGGATATCCAAGGTAAAGTAGATCGTTTAAACTCAGTTGTA
    GATGCTGTTAAAGGTATCGGTGACTCAGTACAAACGTTAAACAGCTCTGTAGATCGTGTA
    ACAAATTCAATTACACATAATATTTCTCAAAATGAAGATAAAATCTCACAAGTTGTTCAA
    TGGTCAAATGTTGCAATGGAAATTGCAGACAAATGGCAAAATAGACACTACCGTCGTGGA
    AGTGCAAATTACAAAGCTAATAATGTAGCAACTGATGCAAATCATAGCTATACTTCTAGA
    GTAGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    492

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1685 [new locus tag: SAUSA300_RS09210 ]
  • symbol: SAUSA300_1685
  • description: hypothetical protein
  • length: 163
  • theoretical pI: 7.67054
  • theoretical MW: 18002.2
  • GRAVY: -0.220245

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Pathogenesis virulence factor Mce family protein (TIGR00996; HMM-score: 33)
    and 7 more
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 14.3)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 14.3)
    helix-rich protein (TIGR04523; HMM-score: 14.1)
    Metabolism Transport and binding proteins Unknown substrate transport protein (TIGR00833; HMM-score: 13.5)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 13.5)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 13.5)
    two transmembrane protein (TIGR04527; HMM-score: 10.8)
  • TheSEED  :
    • UPF0478 protein YtxG
  • PFAM:
    no clan defined DUF948; Bacterial protein of unknown function (DUF948) (PF06103; HMM-score: 98.9)
    and 42 more
    Baculo_PEP_C; Baculovirus polyhedron envelope protein, PEP, C terminus (PF04513; HMM-score: 23.1)
    Laminin_II; Laminin Domain II (PF06009; HMM-score: 21.3)
    Focal_AT; Focal adhesion targeting region (PF03623; HMM-score: 20.4)
    Reo_sigma (CL0326) Reo_sigmaC; Reovirus sigma C capsid protein (PF04582; HMM-score: 19.6)
    no clan defined HsbA; Hydrophobic surface binding protein A (PF12296; HMM-score: 19)
    IncA; IncA protein (PF04156; HMM-score: 17.7)
    Vps51 (CL0295) COG6; Conserved oligomeric complex COG6 (PF06419; HMM-score: 17.5)
    no clan defined Collagen_mid; Bacterial collagen, middle region (PF15984; HMM-score: 16.7)
    Sec34; Sec34-like family (PF04136; HMM-score: 16.5)
    Gp58; gp58-like protein (PF07902; HMM-score: 16.4)
    BORCS6; BLOC-1-related complex sub-unit 6 (PF10157; HMM-score: 16.2)
    STAND_N (CL0587) SesA; N-terminal domain on NACHT_NTPase and P-loop NTPases (PF17107; HMM-score: 16.2)
    no clan defined MCPsignal; Methyl-accepting chemotaxis protein (MCP) signalling domain (PF00015; HMM-score: 16.1)
    DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 15.8)
    FlaC_arch; Flagella accessory protein C (FlaC) (PF05377; HMM-score: 15.7)
    BLOC1_2; Biogenesis of lysosome-related organelles complex-1 subunit 2 (PF10046; HMM-score: 15.6)
    Tweety; Tweety (PF04906; HMM-score: 15.1)
    BORCS8; BLOC-1-related complex sub-unit 8 (PF10167; HMM-score: 15)
    NPV_P10; Nucleopolyhedrovirus P10 protein (PF05531; HMM-score: 14.7)
    MSP1b; Major surface protein 1B (PF03429; HMM-score: 14.2)
    UPF0184; Uncharacterised protein family (UPF0184) (PF03670; HMM-score: 14.2)
    DUF3450; Protein of unknown function (DUF3450) (PF11932; HMM-score: 14.2)
    PDDEXK (CL0236) RmuC; RmuC family (PF02646; HMM-score: 14.1)
    Vps51 (CL0295) COG2; COG (conserved oligomeric Golgi) complex component, COG2 (PF06148; HMM-score: 14.1)
    no clan defined EzrA; Septation ring formation regulator, EzrA (PF06160; HMM-score: 14.1)
    AI-2E_transport; AI-2E family transporter (PF01594; HMM-score: 14)
    Fzo_mitofusin; fzo-like conserved region (PF04799; HMM-score: 13.9)
    Laminin_I; Laminin Domain I (PF06008; HMM-score: 13.8)
    SNARE-fusion (CL0445) Syntaxin_2; Syntaxin-like protein (PF14523; HMM-score: 13.8)
    no clan defined BORCS7; BLOC-1-related complex sub-unit 7 (PF16088; HMM-score: 13.8)
    DUF16; Protein of unknown function DUF16 (PF01519; HMM-score: 13.7)
    Vps51 (CL0295) COG5; Golgi transport complex subunit 5 (PF10392; HMM-score: 13.5)
    no clan defined ApoLp-III; Apolipophorin-III precursor (apoLp-III) (PF07464; HMM-score: 13.2)
    DUF4446; Protein of unknown function (DUF4446) (PF14584; HMM-score: 13.2)
    DivIVA; DivIVA protein (PF05103; HMM-score: 13)
    Cluap1; Clusterin-associated protein-1 (PF10234; HMM-score: 12.6)
    Prominin; Prominin (PF05478; HMM-score: 12.4)
    Chlorosome_CsmC; Chlorosome envelope protein C (PF11098; HMM-score: 12)
    DASH_Dad2; DASH complex subunit Dad2 (PF08654; HMM-score: 11.4)
    GP41; Retroviral envelope protein (PF00517; HMM-score: 11.1)
    OML_zippers (CL0590) LPP; Lipoprotein leucine-zipper (PF04728; HMM-score: 9.8)
    no clan defined Trns_repr_metal; Metal-sensitive transcriptional repressor (PF02583; HMM-score: 8.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.089509
    • TAT(Tat/SPI): 0.00082
    • LIPO(Sec/SPII): 0.071432
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGVEGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTNSITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSRVDK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SAUSA300_1540(dnaK)molecular chaperone DnaK  [1] (data from MRSA252)
    SAUSA300_0993(pdhA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    SAUSA300_0994(pdhB)pyruvate dehydrogenase E1 component, beta subunit  [1] (data from MRSA252)
    SAUSA300_1644(pyk)pyruvate kinase  [1] (data from MRSA252)
    SAUSA300_0523(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SAUSA300_2204(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    SAUSA300_1306(sucA)2-oxoglutarate dehydrogenase E1 component  [1] (data from MRSA252)
    SAUSA300_0995branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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