PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1708
• pan locus tag^?: SAUPAN004448000
• symbol: rot
• pan gene symbol^?: rot
• synonym:
• product: accessory regulator Rot

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1708
• symbol: rot
• product: accessory regulator Rot
• replicon: chromosome
• strand: -
• coordinates: 1890756..1891256
• length: 501
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914258 NCBI
• RefSeq: YP_494402 NCBI
• BioCyc:
• MicrobesOnline: 1293223 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  
  ATGCATAAGTTAGCACATACAAGTTTTGGGATTGTTGGGATGTTTGTTAATACTTGTATA
  GTAGCTAAATATGTGATTATTAATTGGGAGATGTTTAGCATGAAAAAAGTAAATAACGAC
  ACTGTATTTGGAATTTTGCAATTAGAAACACTTTTGGGTGACATTAACTCAATTTTCAGC
  GAGATTGAAAGCGAATACAAAATGTCTAGAGAAGAAATTTTAATTTTACTAACTTTATGG
  CAAAAAGGTTCTATGACGCTTAAAGAAATGGACAGATTTGTTGAAGTTAAACCGTATAAG
  CGTACGAGAACGTATAATAATTTAGTTGAATTAGAATGGATTTACAAAGAGCGTCCTGTT
  GACGATGAAAGAACAGTTATTATTCATTTCAATGAAAAGTTACAACAAGAGAAAGTAGAG
  TTGTTGAATTTCATCAGTGATGCGATTGCAAGTAGAGCAACAGCAATGCAAAATAGTTTA
  AACGCAATTATTGCTGTGTAA
  60
  120
  180
  240
  300
  360
  420
  480
  501
  

⊟Protein

⊟General

• locus tag: SAUSA300_1708
• symbol: Rot
• description: accessory regulator Rot
• length: 166
• theoretical pI: 5.7418
• theoretical MW: 19370.5
• GRAVY: -0.023494

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 76.3)
• TheSEED  :
  • Repressor of toxins Rot
• PFAM:
  HTH (CL0123) MarR; MarR family (PF01047; HMM-score: 32.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.78
  • Cytoplasmic Membrane Score: 8.16
  • Cellwall Score: 0.06
  • Extracellular Score: 0.01
  • Internal Helix: 1
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 4
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.041759
  • TAT(Tat/SPI): 0.000345
  • LIPO(Sec/SPII): 0.131707
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 87161072 NCBI
• RefSeq: YP_494402 NCBI
• UniProt: A0A0H2XFX7 UniProt

⊟Protein sequence

• MHKLAHTSFGIVGMFVNTCIVAKYVIINWEMFSMKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILLTLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQQEKVELLNFISDAIASRATAMQNSLNAIIAV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• data available for NCTC8325

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Hsin-Yeh Hsieh, Ching Wen Tseng, George C Stewart
Regulation of Rot expression in Staphylococcus aureus.
J Bacteriol: 2008, 190(2);546-54
[PubMed:17981965] [WorldCat.org] [DOI] (I p)April Killikelly, Meredith A Benson, Elizabeth A Ohneck, Jared M Sampson, Jean Jakoncic, Brett Spurrier, Victor J Torres, Xiang-Peng Kong
Structure-based functional characterization of repressor of toxin (Rot), a central regulator of Staphylococcus aureus virulence.
J Bacteriol: 2015, 197(1);188-200
[PubMed:25331435] [WorldCat.org] [DOI] (I p)