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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_1722 [new locus tag: SAUSA300_RS09405 ]
- pan locus tag?: SAUPAN004470000
- symbol: SAUSA300_1722
- pan gene symbol?: sigS
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_1722 [new locus tag: SAUSA300_RS09405 ]
- symbol: SAUSA300_1722
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 1904566..1905114
- length: 549
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3914431 NCBI
- RefSeq: YP_494415 NCBI
- BioCyc: see SAUSA300_RS09405
- MicrobesOnline: 1293237 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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481
541ATGAATGATTTTTCTAAATATTTTATTCTAACTTTATCCGGAAATATTTGTTATACGTAT
AACTTGGAGAATGACTACTTGAAATTTAATGACGTATACAACAAACACCACAAAATCATA
CACCATCTTTTAAAAAAATATAATATTAGCTATAATTATGATGAGTATTATCAACTACTC
TTGATAAAAATGTGGCAATTGAGTCAGATATATAAACCCTCAAGCAAGCAATCTTTATCC
TCTTTTTTATTCACTCGATTAAATTTTTACCTTATCGATTTATTCAGACAACAAAATCAA
TTAAAAGATGTCATTTTATGTGAGAATAATTCACCAACATTAACTGAACAACCAACATAC
TTTAATGAACATGACCTTCGTTTACAAGATATCTTCAAGCTTTTAAATCAAAGAGAAAGA
CTATGGCTCAAACTATACCTTGAAGGATACAAGCAATTTGAAATTGCTGAAATCATGTCA
TTATCGCTTTCAACGATTAAATTAATTAAGATGTCCGTTAAGCGTAAATGCCAACATAAT
TTTAATTAG60
120
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549
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_1722 [new locus tag: SAUSA300_RS09405 ]
- symbol: SAUSA300_1722
- description: hypothetical protein
- length: 182
- theoretical pI: 9.30145
- theoretical MW: 22242.6
- GRAVY: -0.436813
⊟Function[edit | edit source]
- TIGRFAM: RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 60.4)and 8 moretranscriptional regulator BotR, P-21 (TIGR03209; HMM-score: 28.5)RNA polymerase sigma factor, SigZ family (TIGR02959; HMM-score: 25.1)RNA polymerase sigma factor, FliA/WhiG family (TIGR02479; HMM-score: 23)RNA polymerase sigma-70 factor, Bacteroides expansion family 1 (TIGR02985; HMM-score: 21.8)RNA polymerase sigma factor, TIGR02999 family (TIGR02999; HMM-score: 14.7)LuxR family transcriptional regulatory, chaperone HchA-associated (TIGR03541; HMM-score: 14.5)RNA polymerase sigma-70 factor, sigma-E family (TIGR02983; HMM-score: 11.4)RNA polymerase sigma-B factor (TIGR02941; HMM-score: 11.3)
- TheSEED :
- RNA polymerase sigma factor SigS
- PFAM: HTH (CL0123) Sigma70_r2; Sigma-70 region 2 (PF04542; HMM-score: 28.5)GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 23.9)and 6 moreSigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 19.6)Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 19.1)HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 12.7)Terminase_5; Putative ATPase subunit of terminase (gpP-like) (PF06056; HMM-score: 12.3)HTH_23; Homeodomain-like domain (PF13384; HMM-score: 11.6)no clan defined DUF3837; Domain of unknown function (DUF3837) (PF12939; HMM-score: 7.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.026255
- TAT(Tat/SPI): 0.000464
- LIPO(Sec/SPII): 0.01734
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNDFSKYFILTLSGNICYTYNLENDYLKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQIYKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRLQDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
Lindsey N Shaw, Catharina Lindholm, Tomasz K Prajsnar, Halie K Miller, Melanie C Brown, Ewa Golonka, George C Stewart, Andrej Tarkowski, Jan Potempa
Identification and characterization of sigma, a novel component of the Staphylococcus aureus stress and virulence responses.
PLoS One: 2008, 3(12);e3844
[PubMed:19050758] [WorldCat.org] [DOI] (I p)