PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1799 [new locus tag: SAUSA300_RS09845 ]
• pan locus tag^?: SAUPAN004782000
• symbol: SAUSA300_1799
• pan gene symbol^?: airS
• synonym:
• product: putative sensor histidine kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1799 [new locus tag: SAUSA300_RS09845 ]
• symbol: SAUSA300_1799
• product: putative sensor histidine kinase
• replicon: chromosome
• strand: -
• coordinates: 1981653..1982765
• length: 1113
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913861 NCBI
• RefSeq: YP_494482 NCBI
• BioCyc: see SAUSA300_RS09845
• MicrobesOnline: 1293314 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGGAACAAAGGACGCGACTAGCTTTATTAAAAGAGATTGCCGAATTTTTAAACGAAGAA
  ACTGAAATGTATAGCATGACCCAAGGTGCGTTAAAATATTTAATTGAGGGCAGTAATTTC
  ACGACAGGCTGGATATTTTTCATCAATAGCGTAGGTGAACATGAACTTGTATCACATGTG
  GCTCTACCACAATCTTTGACAGCAGACCACTGTCACTATATTAAAGATGGGTCTTGTTGG
  TGTGTGAAAGCATTTAATCAACGTAGGCTAATGAAAGCGTCGAATATCGTCAATTGTTCT
  CGTATTAACTTAGCCTCTAAAGCATTTCCTAGCCAAAATGACAATATTACACATCATGCA
  ACGGTACCACTTAAATCGGGTCAAGAACAATTCGGCATTTTAAATGTAGCGTCTCCAAAT
  ACTGAAATATATAGCGATGAAGATTTAGAGTTGCTAGAGTCTGTCGCATTTCAATTAGGG
  TCAGCCATCAAACGTATTTATTTAACAGACCGTGAAAAAGAAGCGGCAAAAATTAATGAA
  CGTAATCGTTTAGCTAGAGATTTACATGATTCAGTAAATCAAATGTTATTTTCTGTAAAA
  CTAACAGCACATGCTGCGTATGGCATGTCAAACGAATCCATCGCTAAACAAGCATTCAAG
  ACGATTGAGGAAACGAGTCAAAATGCCGTTAATGAAATGCGTGCATTGATTTGGCAACTT
  AAGCCAGTTGGACTTGAGCAAGGTTTAATTCATGCTTTGACAGCATATAGTAAATTAATG
  CATATACAATTAAATGTAAATGTAGAAGGTTTAATCGATTTATCTAATGAAATCGAAGAA
  AACATATACAGAGCATTACAAGAGTGTATTAATAATGTTAAGAAACATGCTGATACGAAT
  AAAATGGATCTTACATTAAAACAAATGAACGATATACTATATATTGACGTGATTGATTAT
  GGTCAAGGATTTGAGATCGATAATGTTCAGATAGCTTCATCACACGGTATTAATAACATA
  AAACAAAGAGTTAAATTATTAAGAGGTAAAGTGACCTTTCATTCTCAGCCAACAAAGGGT
  ACACAAATACAATTCACAATTCCTATAAAATAG
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  1113
  

⊟Protein

⊟General

• locus tag: SAUSA300_1799 [new locus tag: SAUSA300_RS09845 ]
• symbol: SAUSA300_1799
• description: putative sensor histidine kinase
• length: 370
• theoretical pI: 6.70362
• theoretical MW: 41880.5
• GRAVY: -0.293784

⊟Function

• TIGRFAM:
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 34.6)
  Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 34.6)
  and 3 more
  Metabolism Central intermediary metabolism Nitrogen fixation Nif-specific regulatory protein (TIGR01817; HMM-score: 25.5)
  Signal transduction Regulatory functions DNA interactions Nif-specific regulatory protein (TIGR01817; HMM-score: 25.5)
  anti-sigma B factor (TIGR01924; EC 2.7.11.1; HMM-score: 12.6)
• TheSEED  :
  • Sensor histidine kinase YhcY
  Nitrogen Metabolism Nitrogen Metabolism - no subcategory Nitrate and nitrite ammonification  Nitrate/nitrite sensor protein (EC 2.7.3.-)
• PFAM:
  His_Kinase_A (CL0025) HisKA_3; Histidine kinase (PF07730; HMM-score: 64.1)
  GAF (CL0161) GAF_2; GAF domain (PF13185; HMM-score: 53)
  and 4 more
  GAF_3; GAF domain (PF13492; HMM-score: 35.8)
  His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 29.2)
  GAF (CL0161) GAF; GAF domain (PF01590; HMM-score: 27.8)
  no clan defined Flu_M1_C; Influenza Matrix protein (M1) C-terminal domain (PF08289; HMM-score: 16.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.05
  • Cytoplasmic Membrane Score: 8.78
  • Cellwall Score: 0.08
  • Extracellular Score: 0.09
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001431
  • TAT(Tat/SPI): 0.000468
  • LIPO(Sec/SPII): 0.000194
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161252 NCBI
• RefSeq: YP_494482 NCBI
• UniProt: A0A0H2XGS2 UniProt

⊟Protein sequence

• MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGWIFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRINLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLGSAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQAFKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSNEIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASSHGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1798 < SAUSA300_1799

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

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⊟Literature

⊟References

⊟Relevant publications