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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1906 [new locus tag: SACOL_RS09815 ]
  • pan locus tag?: SAUPAN004782000
  • symbol: SACOL1906
  • pan gene symbol?: airS
  • synonym:
  • product: sensor histidine kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1906 [new locus tag: SACOL_RS09815 ]
  • symbol: SACOL1906
  • product: sensor histidine kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1961007..1962119
  • length: 1113
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGAACAAAGGACGCGACTAGCTTTATTAAAAGAGATTGCCGAATTTTTAAACGAAGAA
    ACTGAAATGTATAGCATGACCCAAGGTGCGTTAAAATATTTAATTGAGGGCAGTAATTTC
    ACGACAGGCTGGATATTTTTCATCAATAGCGTAGGTGAACATGAACTTGTATCACATGTG
    GCTCTACCACAATCTTTGACAGCAGACCACTGTCACTATATTAAAGATGGGTCTTGTTGG
    TGTGTGAAAGCATTTAATCAACGTAGGCTAATGAAAGCGTCGAATATCGTCAATTGTTCT
    CGTATTAACTTAGCCTCTAAAGCATTTCCTAGCCAAAATGACAATATTACACATCATGCA
    ACGGTACCACTTAAATCGGGTCAAGAACAATTCGGCATTTTAAATGTAGCGTCTCCAAAT
    ACTGAAATATATAGCGATGAAGATTTAGAGTTGCTAGAGTCTGTCGCATTTCAATTAGGG
    TCAGCCATCAAACGTATTTATTTAACAGACCGTGAAAAAGAAGCGGCAAAAATTAATGAA
    CGTAATCGTTTAGCTAGAGATTTACATGATTCAGTAAATCAAATGTTATTTTCTGTAAAA
    CTAACAGCACATGCTGCGTATGGCATGTCAAACGAATCCATCGCTAAACAAGCATTCAAG
    ACGATTGAGGAAACGAGTCAAAATGCCGTTAATGAAATGCGTGCATTGATTTGGCAACTT
    AAGCCAGTTGGACTTGAGCAAGGTTTAATTCATGCTTTGACAGCATATAGTAAATTAATG
    CATATACAATTAAATGTAAATGTAGAAGGTTTAATCGATTTATCTAATGAAATCGAAGAA
    AACATATACAGAGCATTACAAGAGTGTATTAATAATGTTAAGAAACATGCTGATACGAAT
    AAAATGGATCTTACATTAAAACAAATGAACGATATACTATATATTGACGTGATTGATTAT
    GGTCAAGGATTTGAGATCGATAATGTTCAGATAGCTTCATCACACGGTATTAATAACATA
    AAACAAAGAGTTAAATTATTAAGAGGTAAAGTGACCTTTCATTCTCAGCCAACAAAGGGT
    ACACAAATACAATTCACAATTCCTATAAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1113

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1906 [new locus tag: SACOL_RS09815 ]
  • symbol: SACOL1906
  • description: sensor histidine kinase
  • length: 370
  • theoretical pI: 6.70362
  • theoretical MW: 41880.5
  • GRAVY: -0.293784

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 34.6)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 34.6)
    and 3 more
    Metabolism Central intermediary metabolism Nitrogen fixation Nif-specific regulatory protein (TIGR01817; HMM-score: 25.5)
    Signal transduction Regulatory functions DNA interactions Nif-specific regulatory protein (TIGR01817; HMM-score: 25.5)
    anti-sigma B factor (TIGR01924; EC 2.7.11.1; HMM-score: 12.6)
  • TheSEED  :
    • Sensor histidine kinase YhcY
    Nitrogen Metabolism Nitrogen Metabolism - no subcategory Nitrate and nitrite ammonification  Nitrate/nitrite sensor protein (EC 2.7.3.-)
  • PFAM:
    His_Kinase_A (CL0025) HisKA_3; Histidine kinase (PF07730; HMM-score: 64.1)
    GAF (CL0161) GAF_2; GAF domain (PF13185; HMM-score: 53)
    and 4 more
    GAF_3; GAF domain (PF13492; HMM-score: 35.8)
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 29.2)
    GAF (CL0161) GAF; GAF domain (PF01590; HMM-score: 27.8)
    no clan defined Flu_M1_C; Influenza Matrix protein (M1) C-terminal domain (PF08289; HMM-score: 16.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001431
    • TAT(Tat/SPI): 0.000468
    • LIPO(Sec/SPII): 0.000194
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGWIFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRINLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLGSAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQAFKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSNEIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASSHGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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