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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1876 [new locus tag: SAUSA300_RS10265 ]
  • pan locus tag?: SAUPAN004919000
  • symbol: SAUSA300_1876
  • pan gene symbol?: dinB
  • synonym:
  • product: DNA polymerase IV

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1876 [new locus tag: SAUSA300_RS10265 ]
  • symbol: SAUSA300_1876
  • product: DNA polymerase IV
  • replicon: chromosome
  • strand: -
  • coordinates: 2035873..2036913
  • length: 1041
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGACTATTTTTTTGCACAAGTCGAAATGAGAGATAACCCCAAATTGAAAGGGAAACCA
    GTCATTGTTGGTGGTAAAGCAAGCAGTAGGGGTGTTGTCTCTACGGCATCCTATGAAGCA
    CGAAAATACGGCGTCCATTCAGCAATGCCTATGTCACAAGCGCATAAATTATGTCCAAAT
    GGATACTTTGTCACAAGTAATTTTGGTGCTTACCGAGAAACATCTGCGCAGATTATGTCT
    ATTTTCCGAAGTTATACAGATAAGGTAGAACCGATGTCATTGGATGAAGCGTATTTAGAT
    ATTACAGAATTAGTGAGACCAGACCTTCCTGCTTCGAAAATTGCTCAGTATATTAGAAAA
    GATATTCTAGAGCAGACACATTTAACAGCATCTGCAGGTGTTTCTTATAACAAATTTTTA
    GCTAAATTAGCGAGTGGTATGAATAAACCTGATGGTATGACTGTGATTGATTATCAAAAT
    GTCCATGATATTTTGATGACATTGGATATTGGAGATTTTCCAGGCGTAGGTAAAGCTTCC
    AAAAAAGTAATGCATGATAATGGTATTTTTAACGGTAGAGATTTATATGAGAAAACGGAA
    TTTGAATTAATACGTTTGTTTGGAAAAAGAGGTCGGGGTTTATATAACAAGGCACGCGGT
    ATTGACCATAGTGAAGTGAAATCATCAAGAGTAAGAAAATCAGTAGGGACTGAACGCACA
    TTTGCAACAGACGTGAATGATGATGAAGAGATTTTAAGAAAAGTATGGGAATTGTCAGGT
    AAAACAGCTGAACGTCTAAATAAATTACAGAAGTCAGCTAAAACTGTAACGGTTAAAATT
    AAAACTTATCAATTTGAAACGCTATCTAAACAGATGAGTTTAAGAGATTCGGTTAGTTCT
    GAAGAAGATATTTATAATATTGCATATTTACTTTATAACGATTTAAAAGACCCTGATGTA
    CCAATTCGACTTATTGGTGTCACTGTAGGTAATTTAGAACAATCAACTTATAAAAATATG
    ACGATATATGACTTTATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1041

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1876 [new locus tag: SAUSA300_RS10265 ]
  • symbol: SAUSA300_1876
  • description: DNA polymerase IV
  • length: 346
  • theoretical pI: 9.2892
  • theoretical MW: 39049.3
  • GRAVY: -0.432948

Function[edit | edit source]

  • reaction:
    EC 2.7.7.7?  ExPASy
    DNA-directed DNA polymerase Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
  • TIGRFAM:
  • TheSEED:  
    DNA Metabolism DNA repair DNA repair, bacterial  DNA polymerase IV (EC 2.7.7.7)
  • PFAM:
    no clan defined IMS; impB/mucB/samB family (PF00817; HMM-score: 177.3)
    and 3 more
    IMS_C; impB/mucB/samB family C-terminal domain (PF11799; HMM-score: 75.4)
    HHH (CL0198) IMS_HHH; IMS family HHH motif (PF11798; HMM-score: 28.7)
    no clan defined DUF3219; Protein of unknown function (DUF3219) (PF11514; HMM-score: 12.7)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.042379
    • TAT(Tat/SPI): 0.013581
    • LIPO(Sec/SPII): 0.007101
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVSTASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPMSLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKPDGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFGKRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERLNKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRLIGVTVGNLEQSTYKNMTIYDFI

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

  • SAUSA300_1876 no polycistronic organisation predicted

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]