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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_2013 [new locus tag: SAUSA300_RS11070 ]
- pan locus tag?: SAUPAN005310000
- symbol: leuD
- pan gene symbol?: leuD
- synonym:
- product: isopropylmalate isomerase small subunit
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_2013 [new locus tag: SAUSA300_RS11070 ]
- symbol: leuD
- product: isopropylmalate isomerase small subunit
- replicon: chromosome
- strand: +
- coordinates: 2173746..2174318
- length: 573
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3913544 NCBI
- RefSeq: YP_494664 NCBI
- BioCyc: see SAUSA300_RS11070
- MicrobesOnline: 1293528 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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541ATGGCAGCAATCAAACCTATTACAACATATAAAGGTAAAATAGTCCCTCTCTTCAACGAC
AATATCGATACAGACCAAATCATTCCTAAGGTACACTTAAAGCGTATTTCAAAAAGTGGC
TTTGGTCCATTTGCTTTTGATGAATGGCGGTACTTACCTGATGGTTCAGATAATCCTGAT
TTCAATCCTAACAAACCACAATATAAAGGGGCTTCTATTTTAATTACTGGAGATAATTTT
GGATGTGGTTCAAGTCGTGAACATGCTGCTTGGGCTCTTAAGGACTATGGTTTTCATATT
ATTATTGCAGGAAGTTTCAGTGACATATTTTATATGAATTGCACTAAAAATGCGATGTTG
CCTATCGTTTTAGAAAAAAGTGCCCGTGAACATCTTGCACAATATGTTGAAATTGAGGTC
GATTTACCAAATCAAACTGTGTCATCACCAGACAAGCGTTTCCATTTTGAAATTGATGAA
ACTTGGAAGAATAAACTTGTAAATGGCTTAGATGACATTGCAATCACCCTACAATATGAA
TCATTAATAGAAAAATATGAAAAATCACTTTAA60
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573
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_2013 [new locus tag: SAUSA300_RS11070 ]
- symbol: LeuD
- description: isopropylmalate isomerase small subunit
- length: 190
- theoretical pI: 5.63012
- theoretical MW: 21578.4
- GRAVY: -0.356842
⊟Function[edit | edit source]
- reaction: EC 4.2.1.33? ExPASy3-isopropylmalate dehydratase (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate
- TIGRFAM: Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydratase, small subunit (TIGR00171; EC 4.2.1.33; HMM-score: 223.5)and 7 more3-isopropylmalate dehydratase, small subunit (TIGR02087; HMM-score: 83.1)Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydratase, small subunit (TIGR02084; EC 4.2.1.33; HMM-score: 80)Energy metabolism TCA cycle putative aconitate hydratase (TIGR01342; EC 4.2.1.3; HMM-score: 48.1)Amino acid biosynthesis Aspartate family homoaconitase (TIGR00139; EC 4.2.1.36; HMM-score: 40.1)Energy metabolism TCA cycle aconitate hydratase, mitochondrial (TIGR01340; EC 4.2.1.3; HMM-score: 29)Energy metabolism TCA cycle aconitate hydratase 1 (TIGR01341; EC 4.2.1.3; HMM-score: 27.6)2-methylisocitrate dehydratase, Fe/S-dependent (TIGR02333; EC 4.2.1.99; HMM-score: 17.2)
- TheSEED :
- 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33)
Amino Acids and Derivatives Branched-chain amino acids Branched-Chain Amino Acid Biosynthesis 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33)and 1 more - PFAM: Leu-IlvD (CL0364) Aconitase_C; Aconitase C-terminal domain (PF00694; HMM-score: 115.9)and 1 moreAconitase_2_N; Aconitate hydratase 2 N-terminus (PF06434; HMM-score: 12.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002721
- TAT(Tat/SPI): 0.000148
- LIPO(Sec/SPII): 0.000422
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPFAFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIIIAGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYVEIEVDLPNQTVSSPDKRFHFEIDETWKNKLVNGLDDIAITLQYESLIEKYEKSL
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CodY (repression) regulon
CodY (TF) important in Amino acid metabolism; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Charlotte D Majerczyk, Paul M Dunman, Thanh T Luong, Chia Y Lee, Marat R Sadykov, Greg A Somerville, Kip Bodi, Abraham L Sonenshein
Direct targets of CodY in Staphylococcus aureus.
J Bacteriol: 2010, 192(11);2861-77
[PubMed:20363936] [WorldCat.org] [DOI] (I p)