PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2036 [new locus tag: SAUSA300_RS11205 ]
• pan locus tag^?: SAUPAN005352000
• symbol: kdpE
• pan gene symbol^?: kdpE
• synonym:
• product: DNA-binding response regulator, KdpE

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2036 [new locus tag: SAUSA300_RS11205 ]
• symbol: kdpE
• product: DNA-binding response regulator, KdpE
• replicon: chromosome
• strand: +
• coordinates: 2200181..2200876
• length: 696
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913790 NCBI
• RefSeq: YP_494682 NCBI
• BioCyc: see SAUSA300_RS11205
• MicrobesOnline: 1293551 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  
  ATGCAATCTAAAATATTGATAATTGAAGATGATCACGCAATCACACATTTACTTGATGTT
  GCATTAACTTTAGATTATTACAATGTAACTACAGCCGACAATGCCACACAAGCACACTTT
  AAAATTCAAATTGATAAACCAGATGTCATTTTATTAGATTTAGGTTTACCAGATAAAGAT
  GGATTATGTTTGATTTCAGAAATCAGGCAACATACTGACATTCCTATCATTGTAATAAGT
  GCAAGACAAGAAGAACAAACAATTATTCAAGCTTTAGATAACGGTGCGAATGACTATATG
  ACTAAACCTTTTAATGTTGATGAGCTTCGGGCACGAATACGAGTCATCGAGAGAATCGCT
  AAGTCACATCAAGAAACTAACATTGTATTTACTAACGGTTTGCTAAGCATCGACTTTGGC
  TCCAAATCTGTTGTTATTAATAATCAGGAGGTACATTTGACGCCAAATGAATTTAGCTTG
  TTAGAACTATTGTCAAATCATAAAGGAAAAGTACTCACTTATGAGATGATATTAAAACGA
  ATATATGGCTATGTTAATAAGACTGAAATGCCTAGTTTAAGAGTGCATATGACATCATTA
  AGACAAAAACTTTCACAATGTCATGAGGACGCAAAAGATATTATTAAAACACATCCACGC
  ATTGGTTATCAAATGTTGCAGTGGAAAGAGAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  696
  

⊟Protein

⊟General

• locus tag: SAUSA300_2036 [new locus tag: SAUSA300_RS11205 ]
• symbol: KdpE
• description: DNA-binding response regulator, KdpE
• length: 231
• theoretical pI: 6.5114
• theoretical MW: 26511.4
• GRAVY: -0.25671

⊟Function

• reaction:
  EC 2.7.3.-?  ExPASy
• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 164.7)
  Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 164.7)
  and 8 more
  Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 121)
  proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 96.5)
  Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 82.9)
  Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 82.9)
  Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 82.9)
  Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 51.9)
  Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 51.3)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 49)
• TheSEED  :
  • DNA-binding response regulator KdpE
• PFAM:
  CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 100)
  and 3 more
  HTH (CL0123) Trans_reg_C; Transcriptional regulatory protein, C terminal (PF00486; HMM-score: 73.9)
  no clan defined DUF99; Protein of unknown function DUF99 (PF01949; HMM-score: 14.4)
  DUF1463; Protein of unknown function (DUF1463) (PF07316; HMM-score: 13.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002654
  • TAT(Tat/SPI): 0.000144
  • LIPO(Sec/SPII): 0.000299
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160950 NCBI
• RefSeq: YP_494682 NCBI
• UniProt: A0A0H2XG89 UniProt

⊟Protein sequence

• MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQIDKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTKPFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSLLELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTHPRIGYQMLQWKEK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: kdpD > kdpE

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Ting Xue, Yibo You, De Hong, Haipeng Sun, Baolin Sun
The Staphylococcus aureus KdpDE two-component system couples extracellular K+ sensing and Agr signaling to infection programming.
Infect Immun: 2011, 79(6);2154-67
[PubMed:21422185] [WorldCat.org] [DOI] (I p)