PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2046 [new locus tag: SAUSA300_RS11265 ]
• pan locus tag^?: SAUPAN005371000
• symbol: oxaA
• pan gene symbol^?: yidC
• synonym:
• product: membrane protein oxaA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2046 [new locus tag: SAUSA300_RS11265 ]
• symbol: oxaA
• product: membrane protein oxaA
• replicon: chromosome
• strand: -
• coordinates: 2211426..2212298
• length: 873
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914711 NCBI
• RefSeq: YP_494692 NCBI
• BioCyc: see SAUSA300_RS11265
• MicrobesOnline: 1293561 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAAGAAAAAAGCGTTACTACCATTATTTTTAGGTATTATGGTCTTTTTGGCTGGTTGT
  GACTATTCTAAACCTGAAAAACGTAGTGGGTTTTTCTACAATACATTCGTAGATCCAATG
  AAAAATGTATTGGATTGGTTGGGAAATAACTTATTAAACGACAATTATGGTTTAGCTATT
  ATTATCCTTGTATTGGTAATTCGTATTATTTTATTACCATTCATGTTGTCAAACTATAAA
  AATAGTCATATGATGCGTCAAAAAATGAAAGTTGCAAAGCCAGAAGTTGAAAAAATTCAA
  GAAAAAGTGAAACGTGCGCGTACACAAGAAGAAAAAATGGCTGCAAACCAAGAATTAATG
  CAAGTATATAAAAAGTATGACATGAACCCGATTAAGAGTATGTTGGGTTGTTTACCAATG
  CTAATCCAATTACCAATCATCATGGGATTATACTTTGTACTTAAAGACCAACTTGTAGAT
  GGTTTGTTTAAATATCCACACTTCTTATGGTTCGATTTAGGACGTCCTGATATTTGGATT
  ACAATTATTGCCGGTGTTTTATACTTTATCCAAGCATATGTATCAAGTAAAACGATGCCA
  GACGAACAACGTCAAATGGGTTACATGATGATGGTCATTTCACCAATTATGATTATCTGG
  ATTTCATTAAGCTCAGCATCAGCACTTGGTTTGTACTGGTCAGTCAGTGCGGCGTTCCTT
  GTAGTTCAAACACACTTTGCGAACATTTATTATGAAAAAGTCGCTAAAAAAGAAGTACAA
  CCTTTCATTGAAGCGTATGAAAGAGAGCACAACGGCGGCAGCAATAAAAAAGGCAAAAAC
  ACACAAGTTGTGTCTAAAAAGAAAAAGAAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2046 [new locus tag: SAUSA300_RS11265 ]
• symbol: OxaA
• description: membrane protein oxaA
• length: 290
• theoretical pI: 10.1207
• theoretical MW: 33580.9
• GRAVY: 0.0365517

⊟Function

• TIGRFAM:
  Genetic information processing Protein fate Protein and peptide secretion and trafficking membrane protein insertase, YidC/Oxa1 family (TIGR03592; HMM-score: 160.7)
• TheSEED  :
  • Inner membrane protein translocase and chaperone YidC, short form OxaI-like
  Cell Division Subsystem including YidCD  Inner membrane protein translocase component YidC, short form OxaI-like
• PFAM:
  Oxa1 (CL0376) 60KD_IMP; 60Kd inner membrane protein (PF02096; HMM-score: 151.5)
  and 9 more
  no clan defined EI24; Etoposide-induced protein 2.4 (EI24) (PF07264; HMM-score: 20.2)
  DUF4181; Domain of unknown function (DUF4181) (PF13789; HMM-score: 13.9)
  Succ_DH_flav_C; Fumarate reductase flavoprotein C-term (PF02910; HMM-score: 13.8)
  MLANA; Protein melan-A (PF14991; HMM-score: 13.8)
  MFS (CL0015) OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 11.6)
  no clan defined DUF4446; Protein of unknown function (DUF4446) (PF14584; HMM-score: 11.5)
  Trigger_C (CL0262) SurA_N_2; SurA N-terminal domain (PF13623; HMM-score: 10.7)
  Hypoth_1 (CL0447) DUF3784; Domain of unknown function (DUF3784) (PF12650; HMM-score: 8.9)
  no clan defined DUF5325; Family of unknown function (DUF5325) (PF17259; HMM-score: 8.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• LocateP: Multi-transmembrane(Lipid modified N-termini)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: VFLAGCD
• SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
  • SP(Sec/SPI): 0.000365
  • TAT(Tat/SPI): 0.000066
  • LIPO(Sec/SPII): 0.999252
  • Cleavage Site: CS pos: 19-20. LAG-CD. Pr: 0.9999
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI: 87161292 NCBI
• RefSeq: YP_494692 NCBI
• UniProt: Q2FF36 UniProt

⊟Protein sequence

• MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVLDWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEKVKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVDGLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMIIWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNKKGKNTQVVSKKKKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications