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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2140 [new locus tag: SAUSA300_RS11785 ]
  • pan locus tag?: SAUPAN005572000
  • symbol: SAUSA300_2140
  • pan gene symbol?: sfnaD
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2140 [new locus tag: SAUSA300_RS11785 ]
  • symbol: SAUSA300_2140
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2315221..2317197
  • length: 1977
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACTTGAACTTAATTTTTAAAGAACAGACATTAAAATTTAACAAAGAAGAACAAGAA
    ACATATTTATTTTTACAACAACATAATAGCGATTGGGCTAATATTTTTAAAGAGATGATT
    TTACAAGGAAGAGATAAAGTCACTCAGAGATTGGTCACATCAATGCATCGCGAAAACTTA
    GTTAAAGCGCGCACTCAAAGTAAGAAAATATTGAGTCGTGATTTGATTATGTTAGATATC
    TCAACGACTCATATACTAGAAATCCAGTTCCCACAAGCTAAGCAAACATTATATGCTCCT
    ATCACTGGAGAACACGCCTTTGATCGAATTGATGTTGAAGGCCCTTTCTACATTAAAGAT
    GACATTACAAATACGATTACACGAGTACACCATCCAAATGAAATTCTTGAGTGTATTTTA
    ATTGAAGCACCAGATTTAAAAAATGCTGCTAGTGATCAATTTCAGCAAGATTTAATAAAC
    AGTGCAACTAATATGACATTTGCAATTAGCTATCAAGCACTGTCAATGCAACATGATTCT
    GCACCTTTATTTAATATTATCGAAAATAGCGAAGATAGTTACTTGCGTTCAGAGCAAGCC
    GTTATTGAAGGTCATCCGCTACATCCTGGGGCTAAATTACGTAAAGGTTTAAATGCATTA
    CAAACATTCCTATATTCTTCAGAATTCAATCAACCTATTAAGTTGAAAATAGTACTGATT
    CACAGTAAATTAAGCCGTACTATGTCACTGTCTAAAGATTATGATACAACTGTTCATCAA
    CTATTTCCGGACTTAATTAAACAATTAGAAAATGAATTTACGCCAAAGTTTAACTTTAAT
    GATTATCACATTATGATTGTTCATCCATGGCAATTAGATGACGTATTACATTCTGATTAT
    CAAGCAGAAGTAGATAAAGAATTAATCATTGAAGCAAAACATACGTTGGATTATTATGCT
    GGTTTATCTTTCAGAACATTAGTTCCTAAATACCCAGCTATGTCGCCACACATCAAATTG
    TCGACAAACGTTCATATTACAGGTGAGATTCGTACATTATCAGAACAAACGACGCACAAT
    GGTCCACTAATGACACGTATTTTAAATGATATTTTGGAGAAAGATGTCATATTTAAGTCC
    TATGCATCAACAATTATTGATGAAGTTGCTGGTATTCATTTTTATAATGAGCAAGACGAA
    GCAGACTATCAAACTGAACGAAGTGAACAATTAGGTACATTATTTAGAAAAAATATATAC
    CAAATGATTCCACAAGAAGTCACACCATTGATTCCTTCAAGCTTAGTTGCAACCTACCCA
    TTTAACAATGAATCACCTATCGTGACATTGATTAAACGTTATCAATCAGCTGCATCATTG
    TCTGATTTTGAATCATCGGCGAAATCGTGGGTTGAAACTTATAGTAAAGCATTGCTTGGT
    CTCGTAATCCCACTAGTTACTAAATATGGCATTGCATTAGAGGCTCACTTGCAAAATGCT
    ATTGCAACATTTCGGAAAGATGGTCTATTGGACACGATGTATATTAGAGACTTTGAAGGT
    TTAAGAATTGATAAAGCACAACTAAATGAAATGGGATACAGTACATCTCATTTCCATGAA
    AAATCAAGAATTCTTACTGATTCAAAAACGTCTGTGTTTAACAAAGCCTTTTATTCTACC
    GTTCAAAACCATTTAGGCGAATTAATTCTTACAATCTCTAAAGCATCAAATGATTCAAAC
    TTAGAAAGACATATGTGGTATATCGTTCGTGATGTACTAGATAACATTTTTGATCAACTT
    GTACTTTCGACACACAAATCTAATCAAGTAAATGAGAATCGCATAAATGAAATTAAAGAT
    ACCATGTTCGCGCCATTTATAGATTATAAGTGTGTTACAACAATGCGTCTTGAAGATGAG
    GCACATCATTATACTTATATTAAAGTTAATAACCCTTTGTATCGAGAAAATAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1977

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_2140 [new locus tag: SAUSA300_RS11785 ]
  • symbol: SAUSA300_2140
  • description: hypothetical protein
  • length: 658
  • theoretical pI: 6.09975
  • theoretical MW: 76044.8
  • GRAVY: -0.369605

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Siderophore synthetase superfamily, group B
    • Uncharacterized siderophore biosynthesis protein near heme transporter HtsABC
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Uncharacterized siderophore biosynthesis protein near heme transporter HtsABC
  • PFAM:
    PKinase (CL0016) IucA_IucC; IucA / IucC family (PF04183; HMM-score: 194.5)
    and 1 more
    no clan defined FhuF; Ferric iron reductase FhuF-like transporter (PF06276; HMM-score: 106.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001636
    • TAT(Tat/SPI): 0.000276
    • LIPO(Sec/SPII): 0.000233
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGRDKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPITGEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLINSATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLNALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPKFNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAMSPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGIHFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPLIPSSLVATYPFNNESPIVTLIKRYQSAASLSDFESSAKSWVETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDGLLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLGELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFAPFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]