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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2256 [new locus tag: SAUSA300_RS12460 ]
  • pan locus tag?: SAUPAN005782000
  • symbol: SAUSA300_2256
  • pan gene symbol?: —
  • synonym:
  • product: putative N-acetylmuramoyl-L-alanine amidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2256 [new locus tag: SAUSA300_RS12460 ]
  • symbol: SAUSA300_2256
  • product: putative N-acetylmuramoyl-L-alanine amidase
  • replicon: chromosome
  • strand: -
  • coordinates: 2423261..2424037
  • length: 777
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAGAAGAATTTCAAGTTACGCATTTCAACGCTACTATTGATAGTTATTTTAGTTGTT
    TTTGCTGTATTACTCATCGTGAATGAAACTAAATTGTTTAAAAATGATGTGAATTACTCT
    TTTGATGAGGCTGTTTCAATGCAACAAGGGAAAGGTATTGTACAGACAAAAGAAGAGGAT
    GGTAAATTTGTTGAAGCAAATAATAATGAAATTGCTAAAGCAATGACTATTTCACATAAA
    GACAATGATATGAAGTATATGGATATCACAGAAAAAGTGCCAATGTCGGAATCTGAAGTT
    AACCAATTGCTAAAAGGTAAGGGGATTTTAGAAAATCGAGGGAAAGTTTTTCTAGAAGCT
    CAAGAAAAATATGAGGTTAATGTCATTTATCTTGTTAGCCATGCATTAGTAGAAACAGGT
    AACGGCAAATCAGAATTAGCAAAAGGCATTAAAGATGGGAAAAAACGCTATTACAACTTT
    TTTGGTATAGGAGCATTCGATAGTAGTGCTGTTCGTAGTGGGAAAAGTTATGCTGAAAAG
    GAACAATGGACATCACCAGATAAGGCGATTATTGGTGGTGCAAAGTTCATTCGTAATGAA
    TATTTTGAAAACAATCAACTGAATTTATATCAAATGCGATGGAATCCAGAAAATCCTGCG
    CAACATCAATATGCGAGTGACATTCGCTGGGCAGATAAAATTGCCAAATTAATGGATAAA
    TCCTATAAGCAGTTTGGTATAAAGAAAGATGATATTAGACAAACATATTATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    777

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2256 [new locus tag: SAUSA300_RS12460 ]
  • symbol: SAUSA300_2256
  • description: putative N-acetylmuramoyl-L-alanine amidase
  • length: 258
  • theoretical pI: 9.60613
  • theoretical MW: 29671.8
  • GRAVY: -0.555039

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Bifunctional autolysin Atl
    • Endo-beta-N-acetylglucosaminidase (EC 3.2.1.96)
    • N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Murein Hydrolases  N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
    and 3 more
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Recycling of Peptidoglycan Amino Acids  N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
    Regulation and Cell signaling Quorum sensing and biofilm formation Biofilm formation in Staphylococcus  Bifunctional autolysin Atl
    Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Bifunctional autolysin Atl
  • PFAM:
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 63.7)
    and 2 more
    no clan defined Dcc1; Sister chromatid cohesion protein Dcc1 (PF09724; HMM-score: 16.7)
    Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.419066
    • TAT(Tat/SPI): 0.000696
    • LIPO(Sec/SPII): 0.031158
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEAVSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQLLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNFFGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPENPAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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