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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2297 [new locus tag: SAUSA300_RS12690 ]
  • pan locus tag?: SAUPAN005850000
  • symbol: SAUSA300_2297
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2297 [new locus tag: SAUSA300_RS12690 ]
  • symbol: SAUSA300_2297
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2468869..2469507
  • length: 639
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGACAAATCCAAATCAACGATTAGAACCATTTGATGAGACATTTCAACAACCGAATATT
    CATCGTGGTAAGCGATATGGTAAGAAAAAACGTTCATTGGTAAGCATGATTATTCAAATC
    ATTGTTGTTATATTAACCGCAATCGCTGGATACAGCATGTGGAAACAACCCATTTTTAAT
    ATTGTCTTCACCAAAGAATCTGTAGATTTTGATGGCATTAAAAATTTTAACGAGACAATT
    GGAAAAATGCATCAATTAAATATTAATTTGGGTAATATCAATGATTTACAACAAGGAATC
    GATCGCTTAATTTTAGTCTTTAACGGTTTCTTTATTTTATGTATCGTTAGCCTAGTCATA
    ACAATACTGACAATTGTATTTAATCGCACAGTTCTAAAAGTAGTAAATATTTTAGTGTTA
    ACAATTATGTTAGTCATTACATTGTATTTCAGTTATATTATTAAAGCACTCGCACAAGAA
    ATTGCAGATGTTTTCAAAAAATATTATTTAAATGTACCACCAGATCAAGTGATTACAGAA
    GCTGACGCTATTCATAACGGCATCATTTTACTTGGCTGTAGCATCGGTTTACTTGTTATA
    AGCTTATTCTTCCGTAATCGCATGCCTCGTTTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    639

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_2297 [new locus tag: SAUSA300_RS12690 ]
  • symbol: SAUSA300_2297
  • description: hypothetical protein
  • length: 212
  • theoretical pI: 10.0849
  • theoretical MW: 24257.8
  • GRAVY: 0.470755

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EssB (TIGR03926; HMM-score: 9.3)
  • TheSEED  :
    • FIG01108339: hypothetical protein
  • PFAM:
    AmpE_CobD-like (CL0685) CobD_Cbib; CobD/Cbib protein (PF03186; HMM-score: 15)
    PH (CL0266) UCH_N; N-terminal of ubiquitin carboxyl-terminal hydrolase 37 (PF16674; HMM-score: 14.7)
    no clan defined DUF6486; Family of unknown function (DUF6486) (PF20096; HMM-score: 13.5)
    ABHD16_N; Phosphatidylserine Lipase ABHD16 N-terminal domain (PF22990; HMM-score: 13.3)
    CopD_like (CL0430) DUF4149; Domain of unknown function (DUF4149) (PF13664; HMM-score: 12.4)
    and 4 more
    no clan defined DUF2207_C; Predicted membrane protein (DUF2207) C-terminal domain (PF20990; HMM-score: 11.6)
    DUF6442; Family of unknown function (DUF6442) (PF20040; HMM-score: 10.5)
    TMCCDC2; Transmembrane and coiled-coil domain-containing protein 2 (PF15844; HMM-score: 8.1)
    TspO_MBR; TspO/MBR family (PF03073; HMM-score: 6.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0002
    • Cytoplasmic Membrane Score: 0.9427
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0569
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010529
    • TAT(Tat/SPI): 0.000353
    • LIPO(Sec/SPII): 0.004481
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVILTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDRLILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQEIADVFKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]