PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2385 [new locus tag: SAUSA300_RS13195 ]
• pan locus tag^?: SAUPAN005990000
• symbol: SAUSA300_2385
• pan gene symbol^?: kimA
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2385 [new locus tag: SAUSA300_RS13195 ]
• symbol: SAUSA300_2385
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2565941..2567770
• length: 1830
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914943 NCBI
• RefSeq: YP_495020 NCBI
• BioCyc: see SAUSA300_RS13195
• MicrobesOnline: 1293900 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
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  ATGTTCAATCAATTTAAAAGACTTATTATAGGGCAACCTAAAAAAAACAGAGAACTAAAA
  GACGAAAAGATATCGAAGTTTAAAGGGTTAGCGATTCTTTCGTCGGATGCATTATCTTCA
  GTGGCTTACGGTCCCGAACAAATACTGATTACACTATCAGTAGTGGGTGCAGTTGCAACT
  TGGTATACTTTACCGATTGCAGGTGCTGTTCTGATCTTATTAGCTGCTTTAATTATGTCT
  TATAGGCAAATTATTTATGCATATCCTAAAGGTGGCGGCGCGTATATGGTGTCCAAGACC
  AATTTAGGAGAAAAATGGGGATTGCTTGCAGGAGGCTCATTATTAGTCGATTACATACTG
  ACTGTCGCTGTTAGTATATCATCTGGTGCTGATGCATTTGTTGCTGCATTCCCAAGTTTA
  TATGGGCATAAAGTACTGATTGCATGTTTACTTGTACTTTTTATTTTAATATTAAATTTA
  CGTGGTTTAACTGAATCTGCCACCGTATTATCCTATCCAGTATATCTTTTCATTATAGGG
  TTAGTGATATTAATATTTATAGGTACTTTCCGTGTGGCGACAGGAGATATTCAACCACAT
  ATGCATGCATCAGTAGGAACTGCGGTTCCTGGAGTAACATTATTCTTGCTATTAAAAGCA
  TTTTCATCAGGGGCTTCATCGTTAACGGGTGTTGAAGCTATTTCAAATGCGGTAACCAAT
  TTTAGAGAGCCAAGTGCTAAAAATGCTGTGAAAACGTTAATTGCTATGGGTTCAATTTTA
  GCGTTTTTATTAGTTGGTATCGTTGGTTTAGCATATGTTTATGGAATTTTGCCACAAACG
  GAAACGACAGTTTTATCACAATTAGCTATGCAAATATTTGGTGATAATGCTGCCTTCTAT
  TTTGTACAGGCAACGACCGTGATGATTTTGGTATTAGCAGCCAACACTGGATTTACAGCT
  TTTCCGATGTTAGCGGCTTCAATGTCTAAAGATAAATATATGCCTAGAATGTTTACTGTT
  AGAGGTGATCGCTTAGGCTACTCTAACTCGATTATTATTTTAGGTGTATTAGCCATTATT
  TTAATTATTGTATTTGATGGTATGACTGAAGATTTAATTCCATTGTATGCGGTTGGTGTA
  TTTATTCCATTTACACTAGCACAATTCGGTATGGTTATTAAATGGATTCATGAACGTCCT
  AAGAATTGGTTAAGTAAGTTGTCTGTGAACTTACTTGGTGGTATCGTGACATTTATTGTT
  TTTATGATTTTACTCATAACTAAATTTAGCCAAGTATGGCCGATACTTATTTTCTTGCCG
  TTCGTGGTGATTTTCTTCTTGAAAATTAACAAACATTATCGAGATATCGCAGAACAATTA
  CGTTCTGACATTGATGTTCTTAATGTGGACGTGGTAGATCGTAATTTAGCAATTGTGCCG
  ATAACAAGTATTACAACAGCAGTTGATAAGTCTATTTATTACGCACAAATGCTTGCGAAT
  AATGATGTCATAGCAGTACATGTATCATTTGGAGATGAAGATGAAAAAGCATTCCAAGAG
  AAATGGAAACGTCATTTCCCAGATGTAAGATTAGTTATTTTACATTCGGAATATCGCAGT
  ATTATACGACCAATTTCACGTTTTATCGATAAGATTAATCGTAAAGCCAACGATCAAAAC
  TATATGATTACAGTAGTGATACCAGAATTTATTACTAAGAAACGTTGGCATAATTTATTA
  CACAATCAAACTAGTTTGCGTATGAAATTGTATTTAATTTATCAAAAGAATGTTAACGTA
  TGTACAATTCCATTTAAACTTAAAAAATAG
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  1830
  

⊟Protein

⊟General

• locus tag: SAUSA300_2385 [new locus tag: SAUSA300_RS13195 ]
• symbol: SAUSA300_2385
• description: hypothetical protein
• length: 609
• theoretical pI: 10.0029
• theoretical MW: 67572.8
• GRAVY: 0.602791

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 30.1)
  and 1 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 16)
• TheSEED  :
  • Amino acid permease
• PFAM:
  APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 116.7)
  and 2 more
  no clan defined NUFIP1; Nuclear fragile X mental retardation-interacting protein 1 (NUFIP1) (PF10453; HMM-score: 15.3)
  DUF2244; Integral membrane protein (DUF2244) (PF10003; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009533
  • TAT(Tat/SPI): 0.000628
  • LIPO(Sec/SPII): 0.001494
• predicted transmembrane helices (TMHMM): 11

⊟Accession numbers

• GI: 87159897 NCBI
• RefSeq: YP_495020 NCBI
• UniProt: A0A0H2XDX7 UniProt

⊟Protein sequence

• MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYGPEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNLGEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNLRGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLLKAFSSGASSLTGVEAISNAVTNFREPSAKNAVKTLIAMGSILAFLLVGIVGLAYVYGILPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYMPRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMVIKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKINKHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHVSFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVVIPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications