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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2530 [new locus tag: SAUSA300_RS14035 ]
  • pan locus tag?: SAUPAN006275000
  • symbol: SAUSA300_2530
  • pan gene symbol?: —
  • synonym:
  • product: TetR family transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2530 [new locus tag: SAUSA300_RS14035 ]
  • symbol: SAUSA300_2530
  • product: TetR family transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 2731139..2731726
  • length: 588
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACTATAGATCGACGTATTAGAAAATCACAAGTTGCAATGAGAAATGCATTTATTGAA
    CTTTTACATCAACACCAATTAGAAGAGATTACTGTGCAACAAATTGCAGATTTAGCTGAT
    GTTAACCGTAGTACATTTTATACGCATTATTATGATAAGTATGATTTGCTTGAAAAATTA
    GAGGACCAGCAGTTGGAGGAAATAAGAGACTTCATTCATGATGAGAAGTTAAATGGAGCA
    GTTAAGTTATCCACGGATAACATACATCAAATTATGACCGTTTTAATAGAAATGATAGAA
    AAAGACATCGCATTTTATCAATTGATGTTTCGTATGGGTAAAGATTCAAATATCCACGAA
    AAACTTTATGATTTAATTATGTGTCATTTACAACGTTATAAAAATGAAAATGATCAAATT
    AATGGTATACCATTTTCTTATTTCATGAGTTATGTTTCAGGTGCGGGATTGTCTTTTATA
    AGACATTGGGTAGAAGATCCAAACCGTATAGACAAAGTTGACTTAATAAGGCATTTTAAT
    ACGATTGTCAATCAAGGGCCAGCAACGATAATTAAAAATATACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    588

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2530 [new locus tag: SAUSA300_RS14035 ]
  • symbol: SAUSA300_2530
  • description: TetR family transcriptional regulator
  • length: 195
  • theoretical pI: 6.02724
  • theoretical MW: 23193.4
  • GRAVY: -0.404103

⊟Function[edit | edit source]

  • TIGRFAM:
    probable dihydroxyacetone kinase regulator (TIGR02366; HMM-score: 42.8)
    and 4 more
    Signal transduction Regulatory functions DNA interactions mycofactocin system transcriptional regulator (TIGR03968; HMM-score: 25.9)
    Signal transduction Regulatory functions DNA interactions transcriptional repressor BetI (TIGR03384; HMM-score: 21.1)
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 15.6)
    pyrimidine utilization regulatory protein R (TIGR03613; HMM-score: 13.5)
  • TheSEED  :
    • Transcriptional regulator, TetR family
  • PFAM:
    no clan defined TetR_C_8; Transcriptional regulator C-terminal region (PF14278; HMM-score: 38.7)
    and 4 more
    HTH (CL0123) TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 27.7)
    HTH_Tnp_1_2; Helix-turn-helix of insertion element transposase (PF13022; HMM-score: 16.8)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 14.7)
    no clan defined Tim17; Tim17/Tim22/Tim23/Pmp24 family (PF02466; HMM-score: 13.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001938
    • TAT(Tat/SPI): 0.000193
    • LIPO(Sec/SPII): 0.000249
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRSTFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKDIAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFIRHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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