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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS14035 [old locus tag: SAUSA300_2530 ]
- pan locus tag?: SAUPAN006275000
- symbol: SAUSA300_RS14035
- pan gene symbol?: —
- synonym:
- product: TetR/AcrR family transcriptional regulator
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS14035 [old locus tag: SAUSA300_2530 ]
- symbol: SAUSA300_RS14035
- product: TetR/AcrR family transcriptional regulator
- replicon: chromosome
- strand: -
- coordinates: 2731139..2731726
- length: 588
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (2731139..2731726) NCBI
- BioCyc: SAUSA300_RS14035 BioCyc
- MicrobesOnline: see SAUSA300_2530
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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541ATGACTATAGATCGACGTATTAGAAAATCACAAGTTGCAATGAGAAATGCATTTATTGAA
CTTTTACATCAACACCAATTAGAAGAGATTACTGTGCAACAAATTGCAGATTTAGCTGAT
GTTAACCGTAGTACATTTTATACGCATTATTATGATAAGTATGATTTGCTTGAAAAATTA
GAGGACCAGCAGTTGGAGGAAATAAGAGACTTCATTCATGATGAGAAGTTAAATGGAGCA
GTTAAGTTATCCACGGATAACATACATCAAATTATGACCGTTTTAATAGAAATGATAGAA
AAAGACATCGCATTTTATCAATTGATGTTTCGTATGGGTAAAGATTCAAATATCCACGAA
AAACTTTATGATTTAATTATGTGTCATTTACAACGTTATAAAAATGAAAATGATCAAATT
AATGGTATACCATTTTCTTATTTCATGAGTTATGTTTCAGGTGCGGGATTGTCTTTTATA
AGACATTGGGTAGAAGATCCAAACCGTATAGACAAAGTTGACTTAATAAGGCATTTTAAT
ACGATTGTCAATCAAGGGCCAGCAACGATAATTAAAAATATACAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS14035 [old locus tag: SAUSA300_2530 ]
- symbol: SAUSA300_RS14035
- description: TetR/AcrR family transcriptional regulator
- length: 195
- theoretical pI: 6.02724
- theoretical MW: 23193.4
- GRAVY: -0.404103
⊟Function[edit | edit source]
- TIGRFAM: probable dihydroxyacetone kinase regulator (TIGR02366; HMM-score: 42.8)and 4 moreRegulatory functions DNA interactions mycofactocin system transcriptional regulator (TIGR03968; HMM-score: 25.9)Regulatory functions DNA interactions transcriptional repressor BetI (TIGR03384; HMM-score: 21.1)sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 15.6)pyrimidine utilization regulatory protein R (TIGR03613; HMM-score: 13.5)
- TheSEED: see SAUSA300_2530
- PFAM: no clan defined TetR_C_8; Transcriptional regulator C-terminal region (PF14278; HMM-score: 38.7)and 4 moreHTH (CL0123) TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 27.7)HTH_Tnp_1_2; Helix-turn-helix of insertion element transposase (PF13022; HMM-score: 16.8)HTH_23; Homeodomain-like domain (PF13384; HMM-score: 14.7)no clan defined Tim17; Tim17/Tim22/Tim23/Pmp24 family (PF02466; HMM-score: 13.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.001938
- TAT(Tat/SPI): 0.000193
- LIPO(Sec/SPII): 0.000249
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446073257 NCBI
- RefSeq: WP_000151112 NCBI
- UniProt: see SAUSA300_2530
⊟Protein sequence[edit | edit source]
- MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRSTFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKDIAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFIRHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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