PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2558 [new locus tag: SAUSA300_RS14235 ]
• pan locus tag^?: SAUPAN006337000
• symbol: nsaS
• pan gene symbol^?: nsaS
• synonym: braS
• product: nisin susceptibility-associated sensor histidine kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2558 [new locus tag: SAUSA300_RS14235 ]
• symbol: nsaS
• product: nisin susceptibility-associated sensor histidine kinase
• replicon: chromosome
• strand: -
• coordinates: 2769069..2769956
• length: 888
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914230 NCBI
• RefSeq: YP_495192 NCBI
• BioCyc: see SAUSA300_RS14235
• MicrobesOnline: 1294073 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGACCTTTCTTAAAAGTATTACTCAGGAAATAGCAATAGTCATAGTTATTTTTGCTTTG
  TTTGGCTTAATGTTTTACCTGTATCATTTGCCATTAGAAGCATATTTACTAGCACTTGGC
  GTTATTTTATTATTATTACTCATATTCATAGGTATTAAATATTTAAGTTTTGTAAAAACT
  ATAAGCCAACAACAACAAATTGAAAACTTAGAAAATGCGTTGTATCAGCTTAAAAATGAA
  CAAATTGAATATAAAAATGATGTAGAGAGCTACTTTTTAACATGGGTACATCAAATGAAA
  ACACCCATTACTGCAGCACAACTGTTACTTGAAAGAGATGAGCCTAATGTTGTTAATCGT
  GTTCGTCAAGAGGTTATTCAAATTGATAACTATACAAGTTTAGCACTTAGTTATTTAAAG
  TTATTAAATGAAACTTCTGATATTTCTGTCACTAAAATTTCGATTAATAATATCATTCGC
  CCAATTATTATGAAATATTCAATACAGTTTATTGATCAAAAAACAAAAATCCATTATGAA
  CCTTGTCATCACGAAGTATTAACTGACGTTAGATGGACCTCTTTAATGATAGAACAATTA
  ATAAATAATGCACTTAAGTATGCGAGAGGTAAAGATATATGGATTGAATTTGATGAGCAA
  TCCAATCAATTACACGTAAAAGATAATGGTATCGGTATTAGTGAAGCGGACTTGCCTAAA
  ATATTTGATAAGGGCTATTCAGGTTATAATGGCCAGCGCCAAAGTAACTCAAGTGGGATT
  GGTTTATTTATCGTAAAACAAATTTCAACACACACAAACCATCCTGTTTCAGTCGTATCT
  AAACAAAATGAGGGTACAACATTTACGATTCAATTTCCAGATGAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2558 [new locus tag: SAUSA300_RS14235 ]
• symbol: NsaS
• description: nisin susceptibility-associated sensor histidine kinase
• length: 295
• theoretical pI: 6.09533
• theoretical MW: 34019
• GRAVY: -0.0125424

⊟Function

• reaction:
  EC 2.7.3.-?  ExPASy
• TIGRFAM:
  Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 62.9)
  and 8 more
  heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 49.3)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 43.7)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 31.3)
  Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 31.3)
  Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 30.3)
  Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 30.3)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA mismatch repair protein MutL (TIGR00585; HMM-score: 20.1)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA topoisomerase VI, B subunit (TIGR01052; EC 5.99.1.3; HMM-score: 17.5)
• TheSEED  :
  • Putative two-component sensor histidine kinase
• PFAM:
  His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 73.1)
  and 10 more
  HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 26.9)
  HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 14.7)
  no clan defined DUF1043; Protein of unknown function (DUF1043) (PF06295; HMM-score: 14.6)
  Phage_holin_3_6; Putative Actinobacterial Holin-X, holin superfamily III (PF07332; HMM-score: 14.5)
  FtsL (CL0225) DivIC; Septum formation initiator (PF04977; HMM-score: 13.8)
  C_Lectin (CL0056) Ly49; Ly49-like protein, N-terminal region (PF08391; HMM-score: 13.6)
  His_Kinase_A (CL0025) HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 12.5)
  no clan defined DUF4131; Domain of unknown function (DUF4131) (PF13567; HMM-score: 11.8)
  IncA; IncA protein (PF04156; HMM-score: 6.9)
  DUF3290; Protein of unknown function (DUF3290) (PF11694; HMM-score: 6.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 9.99
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 2
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.000379
  • TAT(Tat/SPI): 0.000142
  • LIPO(Sec/SPII): 0.013345
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 87161488 NCBI
• RefSeq: YP_495192 NCBI
• UniProt: A0A0H2XIM9 UniProt

⊟Protein sequence

• MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILLLLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTPITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIRPIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFDEQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPVSVVSKQNEGTTFTIQFPDE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_2556 < SAUSA300_2557 < nsaS < SAUSA300_2559 < SAUSA300_2560

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications