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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_2584 [new locus tag: SAUSA300_RS14370 ]
- pan locus tag?: SAUPAN006382000
- symbol: SAUSA300_2584
- pan gene symbol?: secA2
- synonym:
- product: preprotein translocase subunit SecA
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_2584 [new locus tag: SAUSA300_RS14370 ]
- symbol: SAUSA300_2584
- product: preprotein translocase subunit SecA
- replicon: chromosome
- strand: -
- coordinates: 2803587..2805977
- length: 2391
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3913142 NCBI
- RefSeq: YP_495218 NCBI
- BioCyc: see SAUSA300_RS14370
- MicrobesOnline: 1294099 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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2341ATGAAACATAAACTAGATGTTACGATAAACGAATTAAGACTAAAGTCGATTAGAAAAATA
GTGAAACGCATCAATACATGGAGCGATGAAGTTAAATCATATTCTGATGATGCGTTAAAA
CAAAAGACAATAGAATTTAAAGAACGTTTAGCATCAGGAGTTGATACACTAGATACATTG
TTACCTGAAGCTTATGCAGTGGCACGTGAAGCGAGCTGGAGAGTATTAGGTATGTATCCT
AAAGAAGTTCAGTTAATCGGAGCGATTGTATTGCATGAAGGTAACATTGCGGAGATGCAA
ACAGGCGAAGGTAAAACATTAACGGCAACGATGCCTTTATATTTAAATGCTCTTTCGGGA
AAAGGAACATATTTAATTACAACGAATGATTATTTAGCGAAGCGAGATTTTGAAGAGATG
CAACCATTATATGAATGGTTAGGTTTAACTGCTTCATTAGGTTTTGTTGATATTGTGGAT
TATGAGTATCAAAAAGGGGAAAAACGTAATATATATGAACATGATATTATATATACAACT
AATGGACGTCTCGGTTTTGATTATTTAATTGATAATTTGGCTGATAGTGCGGAAGGGAAA
TTTTTACCACAATTAAATTACGGTATTATTGATGAAGTGGATTCAATCATTTTAGATGCT
GCTCAAACACCATTAGTTATTTCGGGTGCACCAAGATTACAATCAAATCTATTTCATATT
GTGAAAGAGTTTGTAGATACATTGATTGAAGACGTGCATTTTAAAATGAAGAAGACCAAA
AAAGAAATATGGCTGTTAAATCAAGGTATTGAAGCGGCACAATCATACTTTAATGTTGAA
GATTTATATAGCGAACAAGCGATGGTCCTAGTGCGTAATATTAATTTAGCACTGCGCGCA
CAATATTTGTTTGAATCTAATGTCGATTACTTTGTATATAATGGTGATATTGTTTTAATT
GACCGTATTACAGGTCGTATGTTACCGGGAACTAAGTTGCAAGCTGGACTTCACCAAGCT
ATTGAAGCGAAAGAAGGTATGGAGGTTTCAACAGATAAAAGTGTTATGGCAACAATTACC
TTCCAGAATTTATTTAAACTTTTTGAATCATTTTCAGGTATGACAGCTACAGGAAAATTA
GGCGAATCAGAGTTCTTTGATTTGTATTCAAAAATAGTCGTACAAGTACCAACTGATAAA
GCGATTCAACGTATCGATGAACCAGATAAAGTGTTTCGTTCAGTTGATGAGAAAAACATC
GCGATGATTCATGATATAGTTGAACTTCATGAAACGGGGCGACCGGTTTTACTCATAACG
AGAACTGCTGAAGCGGCTGAATACTTTTCGAAAGTATTATTCCAAATGGATATTCCTAAT
AATTTACTCATTGCGCAAAATGTTGCAAAAGAAGCGCAGATGATAGCTGAAGCAGGGCAA
ATTGGTTCCATGACTGTTGCGACTAGTATGGCAGGTCGAGGCACAGATATTAAACTTGGT
GAAGGTGTCGAAGCATTAGGTGGATTAGCTGTTATTATTCATGAACATATGGAAAATAGC
CGTGTAGACAGGCAATTACGTGGTCGTTCTGGTAGACAAGGGGATCCGGGATCATCTTGT
ATATATATTTCACTAGATGATTATTTAGTTAAGCGATGGAGCGATAGTAATTTAGCGGAA
AATAATCAATTATATTCATTAGATGCACAACGATTATCGCAAAGTAATTTGTTTAATCGC
AAAGTTAAGCAAATTGTAGTTAAAGCGCAGCGTATCTCGGAAGAACAAGGGGTTAAAGCT
CGTGAAATGGCTAATGAATTTGAAAAAAGCATTAGTATACAGCGAGATCTTGTATACGAG
GAACGCAATCGAGTTTTAGAAATAGATGATGCTGAGAATCAAGATTTTAAAGCGTTAGCT
AAAGATGTATTTGAAATGTTTGTAAATGAGGAAAAGGTGCTAACAAAATCGCGTGTTGTC
GAGTATATTTATCAAAATTTAAGTTTCCAATTTAATAAAGATGTGGCTTGTGTTAATTTT
AAAGATAAGCAAGCAGTAGTGACATTTTTATTAGAGCAATTTGAAAAGCAATTAGCTTTG
AATCGTAAAAACATGCAAAGTGCATATTATTATAATATTTTCGTTCAAAAAGTCTTTTTG
AAAGCAATTGATTCATGTTGGTTAGAACAAGTCGACTATTTACAACAATTAAAAGCAAGC
GTTAATCAACGACAAAATGGGCAACGTAACGCTATTTTTGAATACCATAGAGTTGCCTTA
GATTCATTTGAAGTTATGACACGAAATATCAAAAAAAGAATGGTGAAAAATATTTGTCAA
AGCATGATTACATTTGATAAAGAGGGTATGCCAGTTATACATTTTCCATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_2584 [new locus tag: SAUSA300_RS14370 ]
- symbol: SAUSA300_2584
- description: preprotein translocase subunit SecA
- length: 796
- theoretical pI: 5.29414
- theoretical MW: 90929.4
- GRAVY: -0.265704
⊟Function[edit | edit source]
- TIGRFAM: Protein fate Protein and peptide secretion and trafficking accessory Sec system translocase SecA2 (TIGR03714; HMM-score: 1241.3)and 3 moreProtein fate Protein and peptide secretion and trafficking preprotein translocase, SecA subunit (TIGR00963; HMM-score: 689.5)Protein fate Protein and peptide secretion and trafficking accessory Sec system translocase SecA2 (TIGR04397; HMM-score: 668.9)accessory Sec system translocase SecA2 (TIGR04221; HMM-score: 484.6)
- TheSEED :
- Protein export cytoplasm protein SecA2 ATPase RNA helicase (TC 3.A.5.1.1)
- PFAM: P-loop_NTPase (CL0023) SecA_DEAD; SecA DEAD-like domain (PF07517; HMM-score: 340.6)and 4 moreno clan defined SecA_PP_bind; SecA preprotein cross-linking domain (PF01043; HMM-score: 91.1)SecA_SW; SecA Wing and Scaffold domain (PF07516; HMM-score: 88.1)P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 27.7)no clan defined Mrx7; MIOREX complex component 7 (PF10906; HMM-score: 12.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.007036
- TAT(Tat/SPI): 0.000872
- LIPO(Sec/SPII): 0.00129
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTIEFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTGEGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVDYEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIILDAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSYFNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQAGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKIVVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFSKVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGGLAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYSLDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNRVLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKDKQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKASVNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
SAUSA300_2142 (asp23) alkaline shock protein 23 [1] (data from MRSA252) SAUSA300_0532 (fusA) elongation factor G [1] (data from MRSA252) SAUSA300_1880 (gatB) aspartyl/glutamyl-tRNA amidotransferase subunit B [1] (data from MRSA252) SAUSA300_1640 (icd) isocitrate dehydrogenase [1] (data from MRSA252) SAUSA300_2541 (mqo) malate:quinone oxidoreductase [1] (data from MRSA252) SAUSA300_0993 (pdhA) pyruvate dehydrogenase E1 component, alpha subunit [1] (data from MRSA252) SAUSA300_0220 (pflB) formate acetyltransferase [1] (data from MRSA252) SAUSA300_1644 (pyk) pyruvate kinase [1] (data from MRSA252) SAUSA300_2203 (rplD) 50S ribosomal protein L4 [1] (data from MRSA252) SAUSA300_2202 (rplW) 50S ribosomal protein L23 [1] (data from MRSA252) SAUSA300_2074 (rpmE2) 50S ribosomal protein L31 type B [1] (data from MRSA252) SAUSA300_1149 (rpsB) 30S ribosomal protein S2 [1] (data from MRSA252) SAUSA300_2198 (rpsC) 30S ribosomal protein S3 [1] (data from MRSA252) SAUSA300_2171 (rpsI) 30S ribosomal protein S9 [1] (data from MRSA252) SAUSA300_0368 (rpsR) 30S ribosomal protein S18 [1] (data from MRSA252) SAUSA300_1305 (sucB) dihydrolipoamide succinyltransferase [1] (data from MRSA252) SAUSA300_1150 (tsf) elongation factor Ts [1] (data from MRSA252) SAUSA300_0533 (tuf) elongation factor Tu [1] (data from MRSA252) SAUSA300_2066 (upp) uracil phosphoribosyltransferase [1] (data from MRSA252) SAUSA300_0658 LysR family transcriptional regulator [1] (data from MRSA252) SAUSA300_0844 hypothetical protein [1] (data from MRSA252) SAUSA300_1656 universal stress protein [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)