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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_2618 [new locus tag: SAUSA300_RS14550 ]
- pan locus tag?: SAUPAN006441000
- symbol: SAUSA300_2618
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_2618 [new locus tag: SAUSA300_RS14550 ]
- symbol: SAUSA300_2618
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 2845219..2845773
- length: 555
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3915064 NCBI
- RefSeq: YP_495252 NCBI
- BioCyc: see SAUSA300_RS14550
- MicrobesOnline: 1294133 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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541ATGAAAAAACAAGATATTTCAGTTAAAACTGTTGTTGCAATTGGTATAGGAGCAGCGGTA
TTTGTAATTTTAGGGCGTTTTGTTGTAATACCAACAGGTTTTCCTAATACGAATATAGAA
ACATCATATGCATTTTTAGCATTAATATCTGCAATATTTGGACCTTTTGCTGGTTTAATG
ACTGGATTAGTTGGACATGCTATTAAAGATTTTACGACATACGGTAGTGCCTGGTGGAGT
TGGGTTATTTGCTCGGGAATTATAGGCTGTTTATATGGCTGGATTGGACTAAAATTAAAT
CTTTCTTCAGGCCGATTTTCTAGGAAATCGATGGTTTATTTTAATATCGGTCAAATTATT
GCGAATATTATTTGCTGGGCACTTATTGCACCAACATTAGATATTTTGATTTATAACGAA
CCAGCTAACAAGGTTTATACACAAGGTGTTATCTCTGCAGTATTAAATATTATTTCAGTT
GGCATTATTGGGACAATATTATTAAAAGCATATGCTTCATCTCAAATTAAAAAAGGTAGT
TTACGTAAGGAATAG60
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555
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_2618 [new locus tag: SAUSA300_RS14550 ]
- symbol: SAUSA300_2618
- description: hypothetical protein
- length: 184
- theoretical pI: 10.0688
- theoretical MW: 19839.4
- GRAVY: 0.747283
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Unknown substrate ECF transporter S component, folate family (TIGR04518; HMM-score: 36.9)
- TheSEED :
- Substrate-specific component MtsA of methionine-regulated ECF transporter
Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis Substrate-specific component MtsA of methionine-regulated ECF transporterand 1 more - PFAM: Gx_transp (CL0315) ECF-ribofla_trS; ECF-type riboflavin transporter, S component (PF07155; HMM-score: 173.3)and 3 moreECF_trnsprt; ECF transporter, substrate-specific component (PF12822; HMM-score: 30.7)no clan defined Glycophorin_A; Glycophorin A (PF01102; HMM-score: 12.7)DUF3021; Protein of unknown function (DUF3021) (PF11457; HMM-score: 7.1)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 5
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: 0
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.043392
- TAT(Tat/SPI): 0.001417
- LIPO(Sec/SPII): 0.012129
- predicted transmembrane helices (TMHMM): 5
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYAFLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLSSGRFSRKSMVYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISVGIIGTILLKAYASSQIKKGSLRKE
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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