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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2709 [new locus tag: SACOL_RS14170 ]
  • pan locus tag?: SAUPAN006441000
  • symbol: SACOL2709
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2709 [new locus tag: SACOL_RS14170 ]
  • symbol: SACOL2709
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2781871..2782425
  • length: 555
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAAAAAACAAGATATTTCAGTTAAAACTGTTGTTGCAATTGGTATAGGAGCAGCGGTA
    TTTGTAATTTTAGGGCGTTTTGTTGTAATACCAACAGGTTTTCCTAATACGAATATAGAA
    ACATCATATGCATTTTTAGCATTAATATCTGCAATATTTGGACCTTTTGCTGGTTTAATG
    ACTGGATTAGTTGGACATGCTATTAAAGATTTTACGACATACGGTAGTGCCTGGTGGAGT
    TGGGTTATTTGCTCGGGAATTATAGGCTGTTTATATGGCTGGATTGGACTAAAATTAAAT
    CTTTCTTCAGGCCGATTTTCTAGGAAATCGATGGTTTATTTTAATATCGGTCAAATTATT
    GCGAATATTATTTGCTGGGCACTTATTGCACCAACATTAGATATTTTGATTTATAACGAA
    CCAGCTAACAAGGTTTATACACAAGGTGTTATCTCTGCAGTATTAAATATTATTTCAGTT
    GGCATTATTGGGACAATATTATTAAAAGCATATGCTTCATCTCAAATTAAAAAAGGTAGT
    TTACGTAAGGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    555

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2709 [new locus tag: SACOL_RS14170 ]
  • symbol: SACOL2709
  • description: hypothetical protein
  • length: 184
  • theoretical pI: 10.0688
  • theoretical MW: 19839.4
  • GRAVY: 0.747283

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Unknown substrate ECF transporter S component, folate family (TIGR04518; HMM-score: 36.9)
  • TheSEED  :
    • Substrate-specific component MtsA of methionine-regulated ECF transporter
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  Substrate-specific component MtsA of methionine-regulated ECF transporter
    and 1 more
    Membrane Transport Membrane Transport - no subcategory ECF class transporters  Substrate-specific component MtsA of methionine-regulated ECF transporter
  • PFAM:
    Gx_transp (CL0315) ECF-ribofla_trS; ECF-type riboflavin transporter, S component (PF07155; HMM-score: 173.3)
    and 3 more
    ECF_trnsprt; ECF transporter, substrate-specific component (PF12822; HMM-score: 30.7)
    no clan defined Glycophorin_A; Glycophorin A (PF01102; HMM-score: 12.7)
    DUF3021; Protein of unknown function (DUF3021) (PF11457; HMM-score: 7.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.043392
    • TAT(Tat/SPI): 0.001417
    • LIPO(Sec/SPII): 0.012129
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYAFLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLSSGRFSRKSMVYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISVGIIGTILLKAYASSQIKKGSLRKE

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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