PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS00200 [old locus tag: SAUSA300_0037 ]
• pan locus tag^?: SAUPAN000444000
• symbol: SAUSA300_RS00200
• pan gene symbol^?: ccrB
• synonym:
• product: recombinase RecB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS00200 [old locus tag: SAUSA300_0037 ]
• symbol: SAUSA300_RS00200
• product: recombinase RecB
• replicon: chromosome
• strand: -
• coordinates: 45892..47520
• length: 1629
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (45892..47520) NCBI
• BioCyc: see SAUSA300_0037
• MicrobesOnline: see SAUSA300_0037

⊟Phenotype

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⊟DNA sequence

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  ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTATCAACAGAAATGCAA
  AGCACAGAAGGTTATAGTATCGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTC
  CATCATTTTGAAGTTAAAGATATATACGCTGACCGTGGTATTTCAGGTAAATCTATGAAC
  AGACCTGAGCTCCAACGTATGTTGAAAGATGCAAAAGAAGGCAATATCGACTGTGTTATG
  GTATACAAAACAAACCGATTAGCTCGTAATACATCTGATCTTCTCAAAATTGTCGAAGAT
  TTACACAAACAAAATGTCGAATTTTTCAGTTTGTCAGAGCGTATGGAAGTCAATACTTCT
  TCTGGTAAACTCATGTTACAGATACTTGCGAGTTTCTCAGAATTCGAACGTAATAACATT
  GTCGAGAACGTATTTATGGGTCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGCAAT
  TTACCACTAGGTTATGACAAAATACCAGATAGTAAACACGAGCTAATGATTAACCAACAT
  GAAGCTAATATTGTAAAATATATATTCGAGTGCTATGCCAAAGGACATGGCTATCGTAAA
  ATTGCCAATGCATTGAATCACAAAGGATATGTCACTAAAAAGGGGAAACCTTTCAGTATT
  AGTTCAATCACATACATCTTAGCTAACCCTTTCTATATCGGCAAAATTCAATTTGCGAAA
  TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTGAATGATAAACCAGTGATAGCTGAA
  GGTAAGCATTCCCCCATTATTAATCAAGATTTATGGGATAAAGTACAAATGCGTAAGAAA
  CAAGTCAGTCAAAAACCCCAAGTCCATGGCAAAGGAACGAATCTGCTTACAGGCATTATT
  CACTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACCACGAATACTCTTAAAGAC
  GGGACTAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAA
  GTATGTTCGGCAAACAGTGTAAGAGCTGATGTGATTGAAGATTATGTGATGAAGCAAATA
  CTTGAAATAGTCAAAAGTGATAAAGTCATTCAACGCGTAGTAACACACGTCAATCAAGAA
  AATCAAGTTGATGGCGCTGCACTTCATCACGATATTGCTTATAAGCAACAACAATATGAT
  GAAGTACAAATCAAAATAAATAACTTGATTAAAACCATCGAGGATAATCCGGACTTAACA
  TCAGTAATCAGACCAAGTATTCAAAAATATGAAAAGCAACTCAATGACATTACGAATCAA
  ATCAACCAACTCAAAAATCAACAAAATGAAGATAAGACTTTATTTGATGCCAAAGAAATC
  AGTAAACTATTACAACACATCTTTCATGATATTAAGCACATAGAAAAATCTCGACTCAAA
  GCATTGTATCTATCAGTGATTGATCGCATTGATATTAAAAAAGATGGTAATCATAAAAAA
  CAATTCTATGTCACACTCAAACTTAATAACGAAATCATTAAACAACTTTTCAATAATAAA
  CAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTATTTTTGCCCCAAACACTATATCTT
  ACTATTTAG
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  1629
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS00200 [old locus tag: SAUSA300_0037 ]
• symbol: SAUSA300_RS00200
• description: recombinase RecB
• length: 542
• theoretical pI: 9.79919
• theoretical MW: 62654.4
• GRAVY: -0.628044

⊟Function

• TIGRFAM:
• TheSEED: see SAUSA300_0037
• PFAM:
  no clan defined Resolvase; Resolvase, N terminal domain (PF00239; HMM-score: 141.9)
  and 12 more
  Recombinase; Recombinase (PF07508; HMM-score: 90)
  Zn_Beta_Ribbon (CL0167) Ogr_Delta; Ogr/Delta-like zinc finger (PF04606; HMM-score: 65.4)
  Zn_ribbon_recom; Recombinase zinc beta ribbon domain (PF13408; HMM-score: 56.6)
  zf-C4_Topoisom; Topoisomerase DNA binding C4 zinc finger (PF01396; HMM-score: 15.1)
  no clan defined Laminin_II; Laminin Domain II (PF06009; HMM-score: 15.1)
  TMPIT; TMPIT-like protein (PF07851; HMM-score: 15)
  HTH (CL0123) HTH_21; HTH-like domain (PF13276; HMM-score: 14.7)
  no clan defined DUF1796; Putative papain-like cysteine peptidase (DUF1796) (PF08795; HMM-score: 12.2)
  Zn_Beta_Ribbon (CL0167) zf-ribbon_3; zinc-ribbon domain (PF13248; HMM-score: 11.8)
  no clan defined Tho2; Transcription factor/nuclear export subunit protein 2 (PF11262; HMM-score: 9.9)
  DUF948; Bacterial protein of unknown function (DUF948) (PF06103; HMM-score: 7.2)
  FlxA; FlxA-like protein (PF14282; HMM-score: 6.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.018476
  • TAT(Tat/SPI): 0.00105
  • LIPO(Sec/SPII): 0.003324
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447109352 NCBI
• RefSeq: WP_001186608 NCBI
• UniProt: see SAUSA300_0037

⊟Protein sequence

• MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGNLPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDIAYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNEDKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications