PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS00275 [old locus tag: SAUSA300_0053 ]
• pan locus tag^?: SAUPAN000807000
• symbol: SAUSA300_RS00275
• pan gene symbol^?: speG
• synonym:
• product: spermidine N1-acetyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS00275 [old locus tag: SAUSA300_0053 ]
• symbol: SAUSA300_RS00275
• product: spermidine N1-acetyltransferase
• replicon: chromosome
• strand: -
• coordinates: 63100..63597
• length: 498
• essential: unknown

⊟Accession numbers

• Location: NC_007793 (63100..63597) NCBI
• BioCyc: SAUSA300_RS00275 BioCyc
• MicrobesOnline: see SAUSA300_0053

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  481
  
  ATGAAACTAAGAGCATTAGAGTATAGTGATTTACTTTTTGTTCATGAATTAAATAATGAA
  TATTCTATTATGTCTTATTGGTTTGAAGAGCCTTATGAATCCTTAACGGAACTTCAACAT
  TTATTTGATAAGCATTTATTAGATGAATCGGAAAGACGTTTTATTGTTGAAGATGAAAAT
  CAAGTCGTAGGTATTGTTGAATTAGTAGAAATTAATTACATCCATCGTAATTGTGAAATA
  CAAATAATTATTAAACCTGAGTTCAGTGGTAAAGGTTATGCAAAATTCGCATTCGAAAAA
  GCGATAATTTATGCATTCAATATTTTAAATATGCACAAGATCTATCTATATGTTGATGCA
  GATAACAAAAAAGCTATTCATATATACGAGTCACAAGGATTTAAAACAGAAGGATTACTT
  AAAGAGCAATTCTACACTAAAGGTAAATATAAAGATGCCTATTTTATGTCATTATTGAAA
  TCTGAATATATTTTGTAG
  60
  120
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  498
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS00275 [old locus tag: SAUSA300_0053 ]
• symbol: SAUSA300_RS00275
• description: spermidine N1-acetyltransferase
• length: 165
• theoretical pI: 5.10639
• theoretical MW: 19780.6
• GRAVY: -0.270303

⊟Function

• reaction:
  EC 2.3.1.57?  ExPASy
  Diamine N-acetyltransferase Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine
• TIGRFAM:
  UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase (TIGR03585; EC 2.3.1.202; HMM-score: 72.5)
  and 3 more
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal-protein-alanine acetyltransferase (TIGR01575; EC 2.3.1.128; HMM-score: 47.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs mycothiol synthase (TIGR03448; EC 2.3.1.189; HMM-score: 27.6)
  Cellular processes Cellular processes Toxin production and resistance aminoglycoside N(6')-acetyltransferase, AacA4 family (TIGR04431; EC 2.3.1.82; HMM-score: 13.8)
• TheSEED: see SAUSA300_0053
• PFAM:
  Acetyltrans (CL0257) Acetyltransf_3; Acetyltransferase (GNAT) domain (PF13302; HMM-score: 65.4)
  Acetyltransf_1; Acetyltransferase (GNAT) family (PF00583; HMM-score: 57.2)
  Acetyltransf_4; Acetyltransferase (GNAT) domain (PF13420; HMM-score: 54.9)
  and 7 more
  Acetyltransf_7; Acetyltransferase (GNAT) domain (PF13508; HMM-score: 38.8)
  Acetyltransf_10; Acetyltransferase (GNAT) domain (PF13673; HMM-score: 35.1)
  Acetyltransf_8; Acetyltransferase (GNAT) domain (PF13523; HMM-score: 25.8)
  GNAT_acetyltran; GNAT acetyltransferase (PF12746; HMM-score: 22.2)
  FR47; FR47-like protein (PF08445; HMM-score: 20.6)
  Acetyltransf_15; Putative acetyl-transferase (PF17013; HMM-score: 16.9)
  no clan defined CBS; CBS domain (PF00571; HMM-score: 13.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001282
  • TAT(Tat/SPI): 0.000074
  • LIPO(Sec/SPII): 0.000139
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446699087 NCBI
• RefSeq: WP_000776433 NCBI
• UniProt: see SAUSA300_0053

⊟Protein sequence

• MKLRALEYSDLLFVHELNNEYSIMSYWFEEPYESLTELQHLFDKHLLDESERRFIVEDENQVVGIVELVEINYIHRNCEIQIIIKPEFSGKGYAKFAFEKAIIYAFNILNMHKIYLYVDADNKKAIHIYESQGFKTEGLLKEQFYTKGKYKDAYFMSLLKSEYIL

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications