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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS00565 [old locus tag: SAUSA300_0109 ]
- pan locus tag?: SAUPAN000903000
- symbol: SAUSA300_RS00565
- pan gene symbol?: —
- synonym:
- product: EamA family transporter
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS00565 [old locus tag: SAUSA300_0109 ]
- symbol: SAUSA300_RS00565
- product: EamA family transporter
- replicon: chromosome
- strand: -
- coordinates: 122939..123811
- length: 873
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (122939..123811) NCBI
- BioCyc: SAUSA300_RS00565 BioCyc
- MicrobesOnline: see SAUSA300_0109
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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841ATGAATAAACTTCAAGACACTACTTTTCTATCATATTTATTTACTATCATATTGTGGGGA
TCTGCATTTCCAATGATAAAGATTGCGTTAAATGATTTTAGTGCAGAATCATTGTCGGCA
TTTCGTTTAATTTTAGCAACAATAATTCTCTTGCCGTTCGTAATTATAAAGAAATTGCCT
ACCCCTGAACTAAGAGATATCCCTGTTATTTTTATTTTAGGATTTTGTGGATTTGTGATA
TATCACACAGCTTTAAATTTTGGTGAAGCTTTGATTAGTGCAGGTATATCTGGAATTCTA
GTCTCTACAACGCCTATTTTTTCTAGTGCTTTAGCTTATATATTTTTAAAAGAGCATTTT
TCAAAATGGAATTGGTTAAGTTCACTCGTCGCATTTATAGGAATATCCATTATTTCAATA
AGTAAAGATGATTACACAACTATTAATGTATTAGGTGTTTTTATTATTTTACTTGCATCT
TTTAGTGAAAGTTTGTATTTTACTTTCCAGAAAAAATACATAGAAAAATACGGCTTCATC
GCTTTCACACTATATACAATAATGGCAAGCTCACCATTTATGCTTATTTTTATTCCTGAA
ATCATCAACGATATACACGGCGCCACTTTTACATCAATAGTATCGGTACTTTATTTAGCT
ATATTCCCTACTATAATTCCATACGTTTTGCTTGCTTATATTGTGAAGTCAGTTGGTGTC
TCTGATGCAACAATGTCTCTTTATTTAACACCTATCGTTTCTTTATTATTATCTTATCTG
TTATTAGACGAGCTACCAACAACCCTTGCTATTATAGGCGGAATTATCACTCTACTAGGC
GTTAGTTTAAGTAACTTCTTTCAAAATACATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS00565 [old locus tag: SAUSA300_0109 ]
- symbol: SAUSA300_RS00565
- description: EamA family transporter
- length: 290
- theoretical pI: 5.64589
- theoretical MW: 32151.1
- GRAVY: 1.0469
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Amino acids, peptides and amines carboxylate/amino acid/amine transporter (TIGR00950; HMM-score: 109.9)and 2 moreHypothetical proteins Conserved Tpt phosphate/phosphoenolpyruvate translocator (TIGR00817; HMM-score: 18.8)Transport and binding proteins Unknown substrate protein RarD (TIGR00688; HMM-score: 15.5)
- TheSEED: see SAUSA300_0109
- PFAM: DMT (CL0184) EamA; EamA-like transporter family (PF00892; HMM-score: 153.9)and 2 moreTPT; Triose-phosphate Transporter family (PF03151; HMM-score: 23.5)EmrE; Putative multidrug resistance efflux transporter (PF13536; HMM-score: 20.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 10
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.028947
- TAT(Tat/SPI): 0.004193
- LIPO(Sec/SPII): 0.011518
- predicted transmembrane helices (TMHMM): 10
⊟Accession numbers[edit | edit source]
- GI: 446960770 NCBI
- RefSeq: WP_001038026 NCBI
- UniProt: see SAUSA300_0109
⊟Protein sequence[edit | edit source]
- MNKLQDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLILATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGEALISAGISGILVSTTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLASFSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLYLAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGIITLLGVSLSNFFQNT
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- data available for N315
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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