Jump to navigation
Jump to search
NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS02085 [old locus tag: SAUSA300_0391 ]
- pan locus tag?: SAUPAN002056000
- symbol: SAUSA300_RS02085
- pan gene symbol?: —
- synonym:
- product: MazF/PemK family toxin
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS02085 [old locus tag: SAUSA300_0391 ]
- symbol: SAUSA300_RS02085
- product: MazF/PemK family toxin
- replicon: chromosome
- strand: -
- coordinates: 442430..443134
- length: 705
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (442430..443134) NCBI
- BioCyc: SAUSA300_RS02085 BioCyc
- MicrobesOnline: see SAUSA300_0391
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541
601
661ATGTCTAAAAATATCAATCAAGCAAATAGTAAATTAAATACGTCAAACAAAAAACTAAAA
CAAGCTTATTCAAAATCCGACAGTAAAAACATGAAAGTTATATACATGCCTCATTGGCTA
GAGTTTTATTCAATTGCTATACATAGCGATGTTACAAGCAATAAAAAAAGATATTATAAA
TCATATAGTAGAGGAACAGTGGTATATGTAAAACTAGGGAGTAATATTGGAAGTGAGTTT
TCTGGTAATCATTTTTGTGTAATATTAGATAACAAAGACAATAAAGGAAAAGAAACAGTT
ACAATAGTTCCACTTTCTTCCAAAGGTAATAAAAATTATTTGAAGTTAAACGAATCGGTG
CTAAATCTAACAGCAACAGATTTAAAAAAACAGATTATCGATATCAGTAGTAAAGTACAA
GCATTAGCCCAAAAATATAGAGAAATTGACATTAAACTTAGCACAGAAGATAAAAAGCTT
TTGTCTAACTTAAATCAAAAAGCTAATGAACTGTATAGAGTGATAGGTGTTTATTCCAAG
CACAAAGGGAAAGATACATACGTTAATATATCAGCAATTACAACAATAAGCAAAAGACGT
ATAAGCAAAATAAATGATTCTGATCCTACTGGAACGATAATAATTTCAGAAGATGACATG
AATGAAATCGAAAAGCAATTAAAGATAAAGTTCTTTTCTAATTGA60
120
180
240
300
360
420
480
540
600
660
705
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS02085 [old locus tag: SAUSA300_0391 ]
- symbol: SAUSA300_RS02085
- description: MazF/PemK family toxin
- length: 234
- theoretical pI: 10.2746
- theoretical MW: 26618.3
- GRAVY: -0.623077
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED: see SAUSA300_0391
- PFAM: CcdB_PemK (CL0624) PemK_toxin; PemK-like, MazF-like toxin of type II toxin-antitoxin system (PF02452; HMM-score: 52.4)and 4 moreGCS (CL0286) Glu_cys_ligase; Glutamate-cysteine ligase (PF04262; HMM-score: 15.6)no clan defined Muted; Organelle biogenesis, Muted-like protein (PF14942; HMM-score: 10)Spc24; Spc24 subunit of Ndc80 (PF08286; HMM-score: 8.3)DASH_Dad4; DASH complex subunit Dad4 (PF08650; HMM-score: 7.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.0158
- TAT(Tat/SPI): 0.000964
- LIPO(Sec/SPII): 0.002292
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 445964028 NCBI
- RefSeq: WP_000041883 NCBI
- UniProt: see SAUSA300_0391
⊟Protein sequence[edit | edit source]
- MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYSIAIHSDVTSNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTIVPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALAQKYREIDIKLSTEDKKLLSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISEDDMNEIEKQLKIKFFSN
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.