PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS03740 [old locus tag: SAUSA300_0697 ]
• pan locus tag^?: SAUPAN002598000
• symbol: SAUSA300_RS03740
• pan gene symbol^?: queC
• synonym:
• product: 7-cyano-7-deazaguanine synthase QueC

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS03740 [old locus tag: SAUSA300_0697 ]
• symbol: SAUSA300_RS03740
• product: 7-cyano-7-deazaguanine synthase QueC
• replicon: chromosome
• strand: -
• coordinates: 771258..771926
• length: 669
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (771258..771926) NCBI
• BioCyc: SAUSA300_RS03740 BioCyc
• MicrobesOnline: see SAUSA300_0697

⊟Phenotype

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⊟DNA sequence

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  ATGGAAAGTGTATTAAATAATGAAAAAGCCATCGTTGTCTTTAGTGGTGGTCAAGACAGT
  ACAACATGTCTCTTTTATGCAAAAAAACATTTCAAAGAAGTTGAACTCGTAACGTTTAAT
  TATGGCCAAAGACATGATACTGAAATTGAAGTCGCAAAACAAATTGCACAAGATCAAGGA
  ATGAAACATCACGTTTTAGATATGTCATTATTATCACAACTTACTCCAAACGCATTAACA
  CAACATGATATGGAAATTACTAATAATGAAGATGGTATACCTAATACATTTGTTCCAGCT
  AGAAATTTACTTTTCTTGTCGTTTGCAGGCGCTCTAGCTTATCAAATTGGGGCTAAGCAT
  ATTATTACAGGCGTATGTGAAACAGACTTTTCAGGCTACCCAGACTGTCGCGATAGTTTT
  ATAAAATCAATGAACGTAACATTAAGCCTAGCTATGGACAAAGATTTTGTCATTCATACT
  CCTTTAATGTGGTTAAACAAAGCAGAAACGTGGAAATTAAGTGATGAACTCGAAGTTTTA
  GATTATATTCGTACAAAAACATTAACATGCTATAACGGTATCATTGGGGATGGCTGTGGT
  GAATGTCCAGCTTGTCATTTACGTCAACGTGGACTAAATCAATATCTTGAAAGTAAAGGA
  GCGCTTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS03740 [old locus tag: SAUSA300_0697 ]
• symbol: SAUSA300_RS03740
• description: 7-cyano-7-deazaguanine synthase QueC
• length: 222
• theoretical pI: 5.27733
• theoretical MW: 24887.2
• GRAVY: -0.186937

⊟Function

• reaction:
  EC 6.3.4.20?  ExPASy
  7-cyano-7-deazaguanine synthase 7-carboxy-7-carbaguanine + NH₃ + ATP = 7-cyano-7-carbaguanine + ADP + phosphate + H₂O
• TIGRFAM:
  Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 278.9)
  and 11 more
  Hypothetical proteins Conserved TIGR00268 family protein (TIGR00268; HMM-score: 26.1)
  Metabolism Amino acid biosynthesis Aspartate family asparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 26)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 23.4)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 21.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 21.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD+ synthetase (TIGR00552; EC 6.3.1.5; HMM-score: 20.7)
  Metabolism Amino acid biosynthesis Glutamate family argininosuccinate synthase (TIGR00032; EC 6.3.4.5; HMM-score: 17.2)
  MJ0570-related uncharacterized domain (TIGR00290; HMM-score: 14.7)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), C-terminal domain (TIGR00884; EC 6.3.5.2; HMM-score: 13.4)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (TIGR00420; EC 2.1.1.61; HMM-score: 12.6)
  Metabolism Central intermediary metabolism Sulfur metabolism sulfate adenylyltransferase, small subunit (TIGR02039; EC 2.7.7.4; HMM-score: 12.5)
• TheSEED: see SAUSA300_0697
• PFAM:
  HUP (CL0039) QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 291.9)
  and 8 more
  Asn_synthase; Asparagine synthase (PF00733; HMM-score: 24.8)
  ATP_bind_3; PP-loop family (PF01171; HMM-score: 21.7)
  NAD_synthase; NAD synthase (PF02540; HMM-score: 19)
  PAPS_reduct; Phosphoadenosine phosphosulfate reductase family (PF01507; HMM-score: 17.8)
  Arginosuc_synth; Arginosuccinate synthase (PF00764; HMM-score: 16.8)
  ThiI; Thiamine biosynthesis protein (ThiI) (PF02568; HMM-score: 15.3)
  Diphthami_syn_2; Diphthamide synthase (PF01902; HMM-score: 14.2)
  TIM_barrel (CL0036) AP_endonuc_2; Xylose isomerase-like TIM barrel (PF01261; HMM-score: 13.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005103
  • TAT(Tat/SPI): 0.000115
  • LIPO(Sec/SPII): 0.000828
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446368869 NCBI
• RefSeq: WP_000446724 NCBI
• UniProt: see SAUSA300_0697

⊟Protein sequence

• MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQRHDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARNLLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHTPLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLESKGAL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: PreQ1 riboswitch see SAUSA300_0697

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications