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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS03930 [old locus tag: SAUSA300_0730 ]
  • pan locus tag?: SAUPAN002653000
  • symbol: SAUSA300_RS03930
  • pan gene symbol?: gdpS
  • synonym:
  • product: GGDEF domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS03930 [old locus tag: SAUSA300_0730 ]
  • symbol: SAUSA300_RS03930
  • product: GGDEF domain-containing protein
  • replicon: chromosome
  • strand: -
  • coordinates: 809991..811061
  • length: 1071
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTTCGAAGCATTTATATACAATATATCTGTTATAGTCGCTGGAATCTATTTATTCCAT
    CGCTTACAGTATTCAGAAAACAAACGTATGGTTTTTTCTAAGGCTTATGTCACTGTTTTA
    ATGACAATCGTATCATTATTATTATCTGTCTACCCTATCCCTTATCGTGAAGATTATTTA
    ATTCACTTAACATTCGTTCCTTTATTGTTTTTAGGTAGATTTACAAATATGGTCTATACA
    TTATCTGCCACAGTAATTGTAGCAATTGTTGAAATTGTAGTATTTAACAACTCGATTATG
    TACGGTGTTACGTTGATTGTTATTGCTGCAGTAACAAGTGCTATTGGACCATTTTTAAAA
    CAAAATGATGTGCTATCATTATTAATACTTAATGTTGTTACAATAATCATTTTATTTGGT
    GTCGCGTTAGTGAGTCCTATTTACACACTATCGGAAGTTATCATTTTAATACCAATTTCA
    TTAATTATCACTTTAGCTTCAGCGATAACATTTGTCGATATTTGGCATTTCTTCTCACTC
    GTAAATCGTTATGAAAACGAAGATAAATATGATTACTTAACAGGCTTAGGTAATGTAAAA
    GAATTTGATAGACATTTAAATGAAATTTCACGAAAAGCTGAAAAAGAACATCAAAGTATC
    GCATTATTATTAATCGATATCGATGGATTTAAAGATGTCAATGATACCTACTCACACAAA
    TCAGGTGATGCTGTATTAAAACAAATGTCTCAATTACTTAAAAACTATGTGCCGAATCAA
    TTTAAAATTTTTAGAAATGGTGGCGAAGAGTTCTCAGTTGTCATTCACAATTATTCACTT
    GATCAAAGTGTTAAATTAGCTGAAAATATTCGTTCGGGTGTTGAAAAATCATCATTCCAT
    TTACCAAACAAAGAAGTTATTAAATTGTCAGTGTCTATCGGTGTTGGTTATTTAACAGAT
    GACGATCCAAAATCACAGCGTAAAGTGTTTAAAGATGCAGACGATATGGTGCATGTAGCC
    AAAAATCAAGGGCGAAACAAAGTAATGTTTAACCCTATTATCAATTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1071

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS03930 [old locus tag: SAUSA300_0730 ]
  • symbol: SAUSA300_RS03930
  • description: GGDEF domain-containing protein
  • length: 356
  • theoretical pI: 7.04024
  • theoretical MW: 40344.8
  • GRAVY: 0.365169

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Signal transduction Other diguanylate cyclase (GGDEF) domain (TIGR00254; HMM-score: 138.7)
    Signal transduction Regulatory functions Small molecule interactions diguanylate cyclase (GGDEF) domain (TIGR00254; HMM-score: 138.7)
  • TheSEED: see SAUSA300_0730
  • PFAM:
    Nucleot_cyclase (CL0276) GGDEF; Diguanylate cyclase, GGDEF domain (PF00990; HMM-score: 122.1)
    and 2 more
    Gx_transp (CL0315) 5TM-5TMR_LYT; 5TMR of 5TMR-LYT (PF07694; HMM-score: 50.3)
    no clan defined DUF2207; Predicted membrane protein (DUF2207) (PF09972; HMM-score: 6.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002442
    • TAT(Tat/SPI): 0.00032
    • LIPO(Sec/SPII): 0.002105
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIVSLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYGVTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPISLIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQSIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIHNYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDADDMVHVAKNQGRNKVMFNPIINL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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