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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS04315 [old locus tag: SAUSA300_0799 ]
- pan locus tag?: SAUPAN002841000
- symbol: SAUSA300_RS04315
- pan gene symbol?: int
- synonym:
- product: site-specific integrase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS04315 [old locus tag: SAUSA300_0799 ]
- symbol: SAUSA300_RS04315
- product: site-specific integrase
- replicon: chromosome
- strand: -
- coordinates: 881996..883216
- length: 1221
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (881996..883216) NCBI
- BioCyc: SAUSA300_RS04315 BioCyc
- MicrobesOnline: see SAUSA300_0799
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1201ATGTGGTTAGAAAAATTTAAAAATAAGAATAAAGAAACTAAATATAGATATTACGAGAAG
TACAAAGACCCTCTCACTGCCAAATGGAGACGTGTTAGCGTGGTACTTAATAAGAATGGT
AAGCAATCACAAAAAGAAGCTCAAAGACTCTTAAATGAGCGTATAGAAGAAAAACTGAAT
GATAAGAAACCTACTACACTTAAGTCACTAACTTTCCATGTCGCATGTGATGAGTGGTTT
CAGAATTATATCAAAACGTCTGGGTCAAAAAGAACAACTATTAAAACTAAATTGAGTAAG
CTAAACACTTTGAAGAAATTTGTAGATGAAGATATTTTAATCAATAAAATAACACTTTCA
TATGCGCAACAAGTTTTCGATGAAATGGATAGTAAAGGTTATGTATATCAAGTAAACAAA
GATGCGTTAAGCATATTCAAAAATGTATTTGAATACACTAGACGTATCTATAAACTGCAA
GATTTAGAATTTTTAAAAGATATAACATTAAATAAAAGAATTAAGTCTTATGATGAAGTG
AAAGCTAAACGTAATAAGTACCTCGAATTAAATGAAATAGAATCTATCATCAAAGATATT
AATACGAAGGCTCAGAAGATGCACTCAGGTATCCATAAGCGGTTTTATTTATTCGTTGCA
CTCATGACAGAATTCCAAGCCTTAAATGGTATGCGTATTGGTGAAATGTTAGCCATTCAA
AATGAAGATATAGACTTTGATAATAAGAGTTTAAATATTAACGGAACAATCCATTGGTTT
CACGATGAATCTGGTGGATTCGGTGTAAAAGATACCACTAAAACAGAGTCTAGTTATAGA
ACAATTGGATTGAGTAGCAGAAGTTGCGAGATATTAAAGAAAGCAATACTGGAGAACAAA
AAGGATTCAAAATGGAATGATGGATATCTAAATAGAAATTTTGTATTTACTAATCATAAA
GGCAACCCAATGCAGACTGAAAGATTCAATAAAATCCTTAGGGAAGCAGCTAAAGATGTT
GGTATTGATAAAGAAGTTTCTTCACATATATTAAGACATAGCCACATATCATTACTCTCT
CAACAAGGCGTGTCACTTAAAGCTATTATGGATCGTGTAGGCCATTCCGACCACCGTACA
ACACTTTCCATTTATTCTCACGTTACTGAACAGATGGATAAGGATATGATGAACAAACTT
GAACAGGTAAAACTTGGATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS04315 [old locus tag: SAUSA300_0799 ]
- symbol: SAUSA300_RS04315
- description: site-specific integrase
- length: 406
- theoretical pI: 10.107
- theoretical MW: 47672.4
- GRAVY: -0.74532
⊟Function[edit | edit source]
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 79.7)and 3 moreDNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 59.1)Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 59.1)DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 58.9)
- TheSEED: see SAUSA300_0799
- PFAM: DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 105.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.010577
- TAT(Tat/SPI): 0.000597
- LIPO(Sec/SPII): 0.005117
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446188303 NCBI
- RefSeq: WP_000266158 NCBI
- UniProt: see SAUSA300_0799
⊟Protein sequence[edit | edit source]
- MWLEKFKNKNKETKYRYYEKYKDPLTAKWRRVSVVLNKNGKQSQKEAQRLLNERIEEKLNDKKPTTLKSLTFHVACDEWFQNYIKTSGSKRTTIKTKLSKLNTLKKFVDEDILINKITLSYAQQVFDEMDSKGYVYQVNKDALSIFKNVFEYTRRIYKLQDLEFLKDITLNKRIKSYDEVKAKRNKYLELNEIESIIKDINTKAQKMHSGIHKRFYLFVALMTEFQALNGMRIGEMLAIQNEDIDFDNKSLNINGTIHWFHDESGGFGVKDTTKTESSYRTIGLSSRSCEILKKAILENKKDSKWNDGYLNRNFVFTNHKGNPMQTERFNKILREAAKDVGIDKEVSSHILRHSHISLLSQQGVSLKAIMDRVGHSDHRTTLSIYSHVTEQMDKDMMNKLEQVKLG
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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