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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS05055 [old locus tag: SAUSA300_0941 ]
- pan locus tag?: SAUPAN003237000
- symbol: SAUSA300_RS05055
- pan gene symbol?: —
- synonym:
- product: ABC transporter substrate-binding protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS05055 [old locus tag: SAUSA300_0941 ]
- symbol: SAUSA300_RS05055
- product: ABC transporter substrate-binding protein
- replicon: chromosome
- strand: +
- coordinates: 1029745..1030704
- length: 960
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (1029745..1030704) NCBI
- BioCyc: SAUSA300_RS05055 BioCyc
- MicrobesOnline: see SAUSA300_0941
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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901GTGAATAGGAATATCGTTAAACTAGTTGTGTTCATGCTAATTTTAGTTGTAGCAGTAGCG
GGTTGTGGTCAAAAAGATACTGAAGAGAAAACTGAAATGACGACAATAAAAGATGAATTA
GGAACTGAAAAAATTAAGAAAAATCCTAAACGTGTTGTTGTATTAGAATATAGTTTTGCT
GATTATTTAGCAGCATTAGATATGAAACCTGTTGGTATTGCAGATGATGGCAGCACTAAA
AATATAACAAAGTCAGTAAGAGATAAGATTGGGGCATATGAATCGGTTGGATCTAGACCG
CAACCGAATATGGAAGTGATAAGTAAATTAAAACCGGATTTGATCATTGCAGATGTTAGC
AGACATAAGAAAATCAAATCAGAATTGAGCAAAATTGCTCCGACAATCATGTTAGTTAGC
GGTACGGGAGATTATAATGCAAATATTGAAGCATTTAAAACAGTCGCTAAAGCAGTAGGC
AAAGAGAAAGAAGGCGAGAAGCGTCTGGAAAAGCATGATAAAATATTAGCGGAGATTAGA
AAGAAAATTGAACAGAGTACGTTAAAATCTGCATTTGCATTCGGTATCTCAAGAGCAGGT
ATGTTTATTAATAATGAAGATACATTTATGGGACAATTCTTAATTAAAATGGGTATTCAA
CCTGAAGTCACAAAAGACAAAACTACGCATGTTGGTGAACGCAAGGGTGGTCCTTATATA
TATTTAAATAATGAAGAACTTGCCAATATCAATCCAAAAGTTATGATTTTAGCCACTGAC
GGAAAAACGGACAAAAATAGAACGAAATTCATTGATCCTGCAGTTTGGAAATCATTAAAA
GCTGTGAAAGATAACAAAGTTTATGACGTTGACCGAAATAAGTGGTTGAAATCAAGGGGG
ATTATCGCAAGTGAAAGTATGGCAGAAGATTTAGAAAAAATTGCAGAAAAAGCAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS05055 [old locus tag: SAUSA300_0941 ]
- symbol: SAUSA300_RS05055
- description: ABC transporter substrate-binding protein
- length: 319
- theoretical pI: 10.0458
- theoretical MW: 35566.2
- GRAVY: -0.410658
⊟Function[edit | edit source]
- TIGRFAM: heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 92.9)and 2 moreputative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 30.1)Transport and binding proteins Amino acids, peptides and amines lipoprotein, PulS/OutS family (TIGR01004; HMM-score: 11.8)
- TheSEED: see SAUSA300_0941
- PFAM: Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 115.9)and 2 moreLppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 15.2)LolA_LolB (CL0048) DUF576; Csa1 family (PF04507; HMM-score: 14.1)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 9.81
- Cellwall Score: 0.09
- Extracellular Score: 0.1
- Internal Helix: 1
- LocateP:
- SignalP: Signal peptide LIPO(Sec/SPII) length 21 aa
- SP(Sec/SPI): 0.000485
- TAT(Tat/SPI): 0.000074
- LIPO(Sec/SPII): 0.999197
- Cleavage Site: CS pos: 21-22. VAG-CG. Pr: 0.9998
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
- GI: 447004095 NCBI
- RefSeq: WP_001081351 NCBI
- UniProt: see SAUSA300_0941
⊟Protein sequence[edit | edit source]
- MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEKIKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQPNMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVGKEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMGIQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVWKSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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