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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS05105 [old locus tag: SAUSA300_0950 ]
  • pan locus tag?: SAUPAN003250000
  • symbol: SAUSA300_RS05105
  • pan gene symbol?: sspB
  • synonym:
  • product: staphopain B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS05105 [old locus tag: SAUSA300_0950 ]
  • symbol: SAUSA300_RS05105
  • product: staphopain B
  • replicon: chromosome
  • strand: -
  • coordinates: 1038279..1039460
  • length: 1182
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAATAGTTCATGTAAATCTAGAGTATTCAATATTATAAGCATCATAATGGTTTCAATG
    CTTATTTTATCACTAGGCGCATTTGCTAATAACAATAAAGCCAAAGCCGATTCACACTCT
    AAACAGCTAGAAATTAATGTTAAGAGTGACAAAGTACCTCAAAAAGTAAAAGATCTAGCA
    CAACAACAATTTGCTGGTTATGCTAAAGCATTAGATAAACAAAGTAATGCAAAAACTGGT
    AAATATGAACTTGGCGAAGCTTTTAAAATTTATAAATTTAATGGTGAAGAAGATAATAGT
    TACTATTATCCAGTTATAAAAGACGGTAAAATTGTTTATACTTTAACACTTAGTCCTAAA
    AATAAAGATGATTTAAACAAATCAAAAGAAGACATGAATTACAGTGTTAAAATTTCAAAC
    TTCATCGCTAAAGATTTAGACCAAATTAAAGATAAAAATTCAAATATCACTGTTCTTACT
    GATGAAAAAGGGTTTTATTTTGAAGAAGATGGCAAAGTTAGATTAGTAAAAGCTACGCCT
    CTACCTGGTAATGTAAAAGAAAAAGAAAGTGCTAAAACAGTTTCAGCAAAATTGAAACAA
    GAGTTAAAAAATACAGTAACACCTACTAAAGTTGAAGAAAACGAAGCGATACAAGAAGAT
    CAAGTTCAATATGAAAATACATTAAAAAACTTCAAAATTAGAGAACAACAATTCGATAAC
    TCATGGTGTGCAGGATTCAGTATGGCAGCATTATTAAATGCAACTAAAAATACAGACACT
    TATAATGCACATGATATTATGCGTACATTATACCCTGAAGTAAGTGAGCAAGACCTTCCT
    AATTGTGCAACATTCCCTAATCAAATGATTGAATACGGTAAATCACAAGGCAGAGATATT
    CATTATCAAGAAGGCGTACCATCATATGAACAAGTTGATCAACTTACAAAAGATAATGTA
    GGAATTATGATCCTTGCACAAAGTGTATCTCAAAACCCTAATGACCCACATTTAGGACAT
    GCGCTAGCAGTTGTTGGTAATGCTAAAATTAATGACCAAGAAAAACTTATTTACTGGAAT
    CCTTGGGATACAGAATTATCAATCCAAGATGCAGATTCAAGCCTATTACATTTATCATTC
    AATCGTGATTATAACTGGTATGGTTCAATGATAGGTTACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1182

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS05105 [old locus tag: SAUSA300_0950 ]
  • symbol: SAUSA300_RS05105
  • description: staphopain B
  • length: 393
  • theoretical pI: 5.75782
  • theoretical MW: 44518.8
  • GRAVY: -0.655471

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.22.48?  ExPASy
    Staphopain Broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate
  • TIGRFAM:
  • TheSEED: see SAUSA300_0950
  • PFAM:
    Peptidase_CA (CL0125) Peptidase_C47; Staphopain peptidase C47 (PF05543; HMM-score: 316.3)
    Cystatin (CL0121) Staphopain_pro; Staphopain proregion (PF14731; HMM-score: 269.1)
    and 1 more
    no clan defined NusG_II; NusG domain II (PF07009; HMM-score: 7.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helix: 1
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 36 aa
    • SP(Sec/SPI): 0.953122
    • TAT(Tat/SPI): 0.01408
    • LIPO(Sec/SPII): 0.023049
    • Cleavage Site: CS pos: 36-37. AKA-DS. Pr: 0.7703
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNSSCKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLEINVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYYYPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLTDEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQEDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSEQDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPNDPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIGY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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