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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS06010 [old locus tag: SAUSA300_1110 ]
- pan locus tag?: SAUPAN003500000
- symbol: SAUSA300_RS06010
- pan gene symbol?: sun
- synonym:
- product: 16S rRNA (cytosine(967)-C(5))-methyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS06010 [old locus tag: SAUSA300_1110 ]
- symbol: SAUSA300_RS06010
- product: 16S rRNA (cytosine(967)-C(5))-methyltransferase
- replicon: chromosome
- strand: +
- coordinates: 1214998..1216305
- length: 1308
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (1214998..1216305) NCBI
- BioCyc: SAUSA300_RS06010 BioCyc
- MicrobesOnline: see SAUSA300_1110
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1261ATGATAGAAAACGTGAGAAGTCTTGCTTTTGACACGATTCAAGATATATTAAATGAAGGT
GCGTATAGTAACTTGCGTATCAATGAAGTGTTGTCAGAAAATGAATTAAATGCAATGGAT
AAGGCTTTATTTACAGAAATTGTCTACGGAACCGTTAAAAGAAAATATACGTTAGATTTT
TATTTAAAGCCTTTTGTGAAAACAAAGATTAAGGCATGGGTTAGGCAATTATTATGGATG
AGTATTTATCAATATGTTTATTTAGATAAAGTTCCAAATCATGCCATTATTAATGAAGCA
GTTGAAATAGCAAAAGAACGCGGTGGCTATCATAATGGTAATGTCGTAAATGGTATTTTA
CGTACAATGATGCGTAGTGACTTACCTGATTTTAATGAAATTGCAGATCCTAAAAAAAGA
ATGGCAATCGAGTATAGTATGCCGAAGTGGATTATAGATCATTGGGCAACACATTATGGT
CTCGAAGAAACTGAAACAATTTTACAGTCATTTTTAGAAACGACATCAACAACTGTGCGT
GCCAACCTGACGCGAGCATCATTAGATGATATTATTGAAAAGTTGCAAGACGAAGGTTAT
GACGTTGAAAAAGATCATGACTTACCTTATTGTCTCCATATAGGAGGACAACCAATTATT
CATTCTCGTTCATTTAAAGATGGATTCGTTTCAATTCAAGATAAAAGCTCAATGTTTGTT
GCACACATTATGAATGTAGACCGACATGATCACGTATTAGATGCATGTAGTGCACCTGGC
GGTAAAGCTTGTCACATTGCTGAAGTTTTAATGCCAGAAGGGCAAGTTGACGCTTCAGAT
ATACATGATCACAAAATAGACTTAATTAATTTTAATATAAAAAAATTACGATTAACAAAT
ATTAAAGCTTTTCAATATGATGCGACAAAACCTTATGATAAAACATACGATAAGATACTT
GTTGATGCACCATGTAGCGGATTAGGTGTAATGAGACATAAGCCGGAGATTAAGTATACT
CAAAGCAAACAACATATTGAGTCACTAGTTGAATTACAGCTTGAAATATTGGAAAATGTA
AAAAACAATGTAAAAATAGGTGGCGAAATCATCTATTCAACATGTACAATTGAGCAACTA
GAAAATGAAAACGTGATTTATACGTTTTTGAAAAATAATAAAAACTTCGAATTTGAACCG
TTTCAACATCCGATAACTGGAGAGTTGGTCAAAACGTTACAAATCATGCCGCAAGACTTT
AATTCAGATGGATTCTTTATCACTAAGATAAAAAGAAAGGACAATTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS06010 [old locus tag: SAUSA300_1110 ]
- symbol: SAUSA300_RS06010
- description: 16S rRNA (cytosine(967)-C(5))-methyltransferase
- length: 435
- theoretical pI: 5.97329
- theoretical MW: 50113
- GRAVY: -0.364368
⊟Function[edit | edit source]
- reaction: EC 2.1.1.176? ExPASy16S rRNA (cytosine967-C5)-methyltransferase S-adenosyl-L-methionine + cytosine967 in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA
- TIGRFAM: Protein synthesis tRNA and rRNA base modification 16S rRNA (cytosine(967)-C(5))-methyltransferase (TIGR00563; EC 2.1.1.176; HMM-score: 394)and 12 moreProtein synthesis tRNA and rRNA base modification NOL1/NOP2/sun family putative RNA methylase (TIGR00446; EC 2.1.1.-; HMM-score: 152.1)Transcription Transcription factors transcription antitermination factor NusB (TIGR01951; HMM-score: 57.4)Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 25.2)Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 18.5)Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 17.2)Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone demethylmenaquinone methyltransferase (TIGR02752; EC 2.1.1.163; HMM-score: 17.2)Protein synthesis tRNA and rRNA base modification N2,N2-dimethylguanosine tRNA methyltransferase (TIGR00308; EC 2.1.1.-; HMM-score: 16.8)Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 16.7)Regulatory functions DNA interactions biotin operon repressor (TIGR00122; HMM-score: 13.4)Protein fate Protein modification and repair protein-L-isoaspartate O-methyltransferase (TIGR00080; EC 2.1.1.77; HMM-score: 13.3)Protein synthesis tRNA and rRNA base modification ribosomal RNA large subunit methyltransferase J (TIGR00438; EC 2.1.1.166; HMM-score: 12.4)methyltransferase, FxLD system (TIGR04364; HMM-score: 10.5)
- TheSEED: see SAUSA300_1110
- PFAM: NADP_Rossmann (CL0063) Methyltr_RsmB-F; 16S rRNA methyltransferase RsmB/F (PF01189; HMM-score: 175.8)and 9 moreNusB (CL0633) NusB; NusB family (PF01029; HMM-score: 108.2)NADP_Rossmann (CL0063) Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 33.4)FtsJ; FtsJ-like methyltransferase (PF01728; HMM-score: 17.5)DUF938; Protein of unknown function (DUF938) (PF06080; HMM-score: 14.4)DUF2431; Domain of unknown function (DUF2431) (PF10354; HMM-score: 14.4)Ubiquitin (CL0072) NLE; NLE (NUC135) domain (PF08154; HMM-score: 14)NADP_Rossmann (CL0063) Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 13.4)Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 12.6)TRM; N2,N2-dimethylguanosine tRNA methyltransferase (PF02005; HMM-score: 12.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002253
- TAT(Tat/SPI): 0.000171
- LIPO(Sec/SPII): 0.000532
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446494382 NCBI
- RefSeq: WP_000572236 NCBI
- UniProt: see SAUSA300_1110
⊟Protein sequence[edit | edit source]
- MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALFTEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVEIAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYGLEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQPIIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQVDASDIHDHKIDLINFNIKKLRLTNIKAFQYDATKPYDKTYDKILVDAPCSGLGVMRHKPEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKNNKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAUSA300_RS03530 LysR family transcriptional regulator [1] (data from MRSA252) SAUSA300_RS04885 NAD(+) kinase [1] (data from MRSA252) SAUSA300_RS05350 pyruvate dehydrogenase E1 component subunit alpha [1] (data from MRSA252) SAUSA300_RS05355 pyruvate dehydrogenase E1 component subunit beta [1] (data from MRSA252) SAUSA300_RS05360 dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex [1] (data from MRSA252) SAUSA300_RS05365 dihydrolipoyl dehydrogenase [1] (data from MRSA252) SAUSA300_RS07100 dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex [1] (data from MRSA252) SAUSA300_RS08910 aldehyde dehydrogenase [1] (data from MRSA252) SAUSA300_RS08950 isocitrate dehydrogenase (NADP(+)) [1] (data from MRSA252) SAUSA300_RS08975 pyruvate kinase [1] (data from MRSA252) SAUSA300_RS12030 30S ribosomal protein S13 [1] (data from MRSA252) SAUSA300_RS12120 30S ribosomal protein S3 [1] (data from MRSA252) SAUSA300_RS13745 pyruvate oxidase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)