From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS07325 [old locus tag: SAUSA300_1343 ]
  • pan locus tag?: SAUPAN003912000
  • symbol: SAUSA300_RS07325
  • pan gene symbol?: nth
  • synonym:
  • product: endonuclease III

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS07325 [old locus tag: SAUSA300_1343 ]
  • symbol: SAUSA300_RS07325
  • product: endonuclease III
  • replicon: chromosome
  • strand: -
  • coordinates: 1508025..1508684
  • length: 660
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGTAAGTAAGAAAAAAGCATTAGAAATGATTGATGTTATAGCAAATATGTTTCCGGAT
    GCAGAATGTGAATTAAAACATGATAATCCGTTCGAATTAACTATTGCTGTATTATTGTCA
    GCGCAATGTACAGACGTTCTAGTAAATAGAGTAACAACTGAGCTGTTTAAAAAATACAAA
    ACGCCTGAAGATTATTTGGCAGTCAGTGATGAAGAACTTATGAATGATATTCGTTCTATC
    GGTCTGTATCGTAACAAAGCTAAGAATATTAAGAAACTTTGTCAATCTTTGATTGATCAA
    TTTAATGGAGAAATACCACAAACACATAAGGAATTAGAAAGTTTAGCAGGTGTAGGACGT
    AAAACTGCTAATGTAGTCATGAGTGTAGCATTTGATGAACCTTCATTAGCTGTTGATACG
    CATGTAGAACGTGTTTCTAAACGCTTAGGTATTAATCGTTGGAAAGATAATGTGAGACAG
    GTAGAAGACCGTTTATGTTCTGTCATACCTAGAGATAGATGGAATAGGAGCCATCATCAA
    CTCATTTTCTTTGGAAGATACCACTGTTTAGCCCGAAAACCTAAATGCGATATTTGTCCA
    CTATTAGAAGATTGTAGAGAAGGACAAAAACGTTATAAAGCTAGTTTGAAAGAAGCGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS07325 [old locus tag: SAUSA300_1343 ]
  • symbol: SAUSA300_RS07325
  • description: endonuclease III
  • length: 219
  • theoretical pI: 8.39452
  • theoretical MW: 25234
  • GRAVY: -0.456621

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.99.18?  ExPASy
    DNA-(apurinic or apyrimidinic site) lyase The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair endonuclease III (TIGR01083; EC 4.2.99.18; HMM-score: 273.8)
    and 3 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair A/G-specific adenine glycosylase (TIGR01084; EC 3.2.2.-; HMM-score: 89)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair 8-oxoguanine DNA-glycosylase (ogg) (TIGR00588; EC 3.2.2.-; HMM-score: 21.6)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 14)
  • TheSEED: see SAUSA300_1343
  • PFAM:
    HHH (CL0198) HhH-GPD; HhH-GPD superfamily base excision DNA repair protein (PF00730; HMM-score: 82.1)
    and 4 more
    HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 33.7)
    no clan defined EndIII_4Fe-2S; Iron-sulfur binding domain of endonuclease III (PF10576; HMM-score: 23.9)
    DUF2400; Protein of unknown function (DUF2400) (PF09674; HMM-score: 12.6)
    P-loop_NTPase (CL0023) AAA_32; AAA domain (PF13654; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: [4Fe-4S] cluster
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.019353
    • TAT(Tat/SPI): 0.000335
    • LIPO(Sec/SPII): 0.007391
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCTDVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFNGEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQVEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKEA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_RS07325&oldid=99945"