From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS07330 [old locus tag: SAUSA300_1344 ]
  • pan locus tag?: SAUPAN003913000
  • symbol: SAUSA300_RS07330
  • pan gene symbol?: dnaD
  • synonym:
  • product: chromosome replication protein DnaD

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS07330 [old locus tag: SAUSA300_1344 ]
  • symbol: SAUSA300_RS07330
  • product: chromosome replication protein DnaD
  • replicon: chromosome
  • strand: -
  • coordinates: 1508674..1509360
  • length: 687
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGATAAATATCAATTAAAAGCAAGACCTGTAGTGATACGAAGAGAATTATTAGACCAT
    TACAGTGACTTAGGTTTAGATGAGCAAGATTTAGTCATTTTGCTTAAATTGATTTATGCT
    TCCGAAACATCAAATAAACAGCCTTCAATTGAATTGCTTCAGAAGGGCTCAACTATGCAA
    CCACGTGATATTACAATGGTTATTCAAAATTTGATACAACGTGAATTGCTAGAATTACAA
    GTTCAAAAAGATGAAGAAGGTAGATTTACCGAATATATGAATCTAGATCCTTTTTTCGAA
    AAATTAAGTCATATATTGAAGCAACAAAGTATGGAAACGAAAGAGCTAAATAGTAAAGAA
    AAATTTAAACAATTATTTAGAGTTTTAGAGGATACATTTGCAAGGCCGTTATCTCCATAT
    GAAATAGAAACGTTAAATCAATGGATTGATGTTGATAAACACGATACTGCAATTATACAA
    GCGGCATTAGACGAGGCGAATAGTTTGAATAAACTTAGCTTTAAATATATAGATCGTATT
    TTACTTAATTGGAAGAAAAACAATGTCAAAACAATTGACGATTCTAGAAAAATAAGAGAA
    AAATTCAATAAACCGAAAATGACGCACACAGTTAAAACGGTACCTAAATTTGATTGGTTA
    AACGGGGAGAACCTTGATGGTAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    687

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS07330 [old locus tag: SAUSA300_1344 ]
  • symbol: SAUSA300_RS07330
  • description: chromosome replication protein DnaD
  • length: 228
  • theoretical pI: 6.98192
  • theoretical MW: 26954.8
  • GRAVY: -0.657456

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaD domain protein (TIGR01446; HMM-score: 78.3)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions DnaD domain protein (TIGR01446; HMM-score: 78.3)
    and 2 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaD family protein (TIGR04548; HMM-score: 39)
    Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 10.6)
  • TheSEED: see SAUSA300_1344
  • PFAM:
    no clan defined DnaB_2; Replication initiation and membrane attachment (PF07261; HMM-score: 76.7)
    and 1 more
    Membr_traf_MHD; Munc13 (mammalian uncoordinated) homology domain (PF10540; HMM-score: 15.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002042
    • TAT(Tat/SPI): 0.000433
    • LIPO(Sec/SPII): 0.000278
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETSNKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKLSHILKQQSMETKELNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQAALDEANSLNKLSFKYIDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFDWLNGENLDGK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_RS07330&oldid=91636"