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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS08030 [old locus tag: SAUSA300_1471 ]
- pan locus tag?: SAUPAN004077000
- symbol: SAUSA300_RS08030
- pan gene symbol?: xseB
- synonym:
- product: exodeoxyribonuclease 7 small subunit
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS08030 [old locus tag: SAUSA300_1471 ]
- symbol: SAUSA300_RS08030
- product: exodeoxyribonuclease 7 small subunit
- replicon: chromosome
- strand: -
- coordinates: 1626365..1626595
- length: 231
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (1626365..1626595) NCBI
- BioCyc: SAUSA300_RS08030 BioCyc
- MicrobesOnline: see SAUSA300_1471
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181ATGACTAAAGAAACGCAAAGTTTTGAAGAAATGATGCAAGAATTAGAGCAAATTGTTCAA
AAATTAGATAATGAAACAGTATCTTTAGAGGAATCATTAGATTTATATCAACGTGGTATG
AAACTATCAGCAGCTTGTGACACAACTTTAAAAAATGCCGAAAAAAAGGTGAATGACTTA
ATAAAAGAAGAAGCTGAGGATGTAAAAAATGACGAATCTACCGATGAATAA60
120
180
231
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS08030 [old locus tag: SAUSA300_1471 ]
- symbol: SAUSA300_RS08030
- description: exodeoxyribonuclease 7 small subunit
- length: 76
- theoretical pI: 3.9341
- theoretical MW: 8759.64
- GRAVY: -0.977632
⊟Function[edit | edit source]
- reaction: EC 3.1.11.6? ExPASyExodeoxyribonuclease VII Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates
- TIGRFAM: DNA metabolism Degradation of DNA exodeoxyribonuclease VII, small subunit (TIGR01280; EC 3.1.11.6; HMM-score: 81)and 2 moreCell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides polysaccharide chain length determinant protein, PEP-CTERM locus subfamily (TIGR03007; HMM-score: 11.3)Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobaltochelatase, CobN subunit (TIGR02257; EC 6.6.1.2; HMM-score: 11)
- TheSEED: see SAUSA300_1471
- PFAM: no clan defined Exonuc_VII_S; Exonuclease VII small subunit (PF02609; HMM-score: 76.1)and 6 moreDASH_Dad2; DASH complex subunit Dad2 (PF08654; HMM-score: 13.8)ADIP; Afadin- and alpha -actinin-Binding (PF11559; HMM-score: 13.2)DUF4359; Domain of unknown function (DUF4359) (PF14271; HMM-score: 12.8)DUF1657; Protein of unknown function (DUF1657) (PF07870; HMM-score: 12.7)DUF2120; Uncharacterized protein conserved in archaea (DUF2120) (PF09893; HMM-score: 12)Med4; Vitamin-D-receptor interacting Mediator subunit 4 (PF10018; HMM-score: 10.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.009855
- TAT(Tat/SPI): 0.035215
- LIPO(Sec/SPII): 0.000826
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446082010 NCBI
- RefSeq: WP_000159865 NCBI
- UniProt: see SAUSA300_1471
⊟Protein sequence[edit | edit source]
- MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSAACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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