PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS08145 [old locus tag: SAUSA300_1492 ]
• pan locus tag^?: SAUPAN004108000
• symbol: SAUSA300_RS08145
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS08145 [old locus tag: SAUSA300_1492 ]
• symbol: SAUSA300_RS08145
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1646167..1646748
• length: 582
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (1646167..1646748) NCBI
• BioCyc: SAUSA300_RS08145 BioCyc
• MicrobesOnline: see SAUSA300_1492

⊟Phenotype

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⊟DNA sequence

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  ATGAAAAAATTGGTTTCAATTGTTGGCGCAACATTATTGTTAGCTGGATGTGGATCACAA
  AATTTAGCACCATTAGAAGAAAAAACAACAGATTTAAGAGAAGATAATCATCAACTCAAA
  CTAGATATTCAAGAACTTAATCAACAAATTAGTGATTCTAAATCTAAAATTAAAGGGCTT
  GAAAAGGATAAAGAAAACAGTAAAAAAACTGCATCTAATAATACGAAAATTAAATTGATG
  AATGTTACATCAACATACTACGACAAAGTTGCTAAAGCTTTGAAATCCTATAACGATATT
  GAGAAAGATGTAAGTAAAAACAAAGGCGATAAGAATGTTCAATCGAAATTAAATCAAATT
  TCTAATGATATTCAAAGTGCTCACACTTCATACAAAGATGCTATCGATGGTTTATCACTT
  AGTGATGATGATAAAAAAACGTCTAAAAATATCGATAAATTAAACTCTGATTTGAATCAT
  GCATTTGATGATATTAAAAATGGCTATCAAAATAAAGATAAAAAACAACTTACAAAAGGA
  CAACAAGCGTTGTCAAAATTAAACTTAAATGCAAAATCATGA
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  582
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS08145 [old locus tag: SAUSA300_1492 ]
• symbol: SAUSA300_RS08145
• description: hypothetical protein
• length: 193
• theoretical pI: 9.8465
• theoretical MW: 21472
• GRAVY: -0.999482

⊟Function

• TIGRFAM:
  SH3 domain protein (TIGR04211; HMM-score: 14.2)
  and 6 more
  Cellular processes Cellular processes Pathogenesis type III secretion apparatus needle protein (TIGR02105; HMM-score: 11.3)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type III secretion apparatus needle protein (TIGR02105; HMM-score: 11.3)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 11.2)
  Genetic information processing Mobile and extrachromosomal element functions Plasmid functions integrating conjugative element protein, PFL_4705 family (TIGR03752; HMM-score: 7.3)
  phage lysis regulatory protein, LysB family (TIGR03495; HMM-score: 5.8)
  Metabolism Energy metabolism Photosynthesis photosystem II protein PsbQ (TIGR03042; HMM-score: 5.1)
• TheSEED: see SAUSA300_1492
• PFAM:
  Transthyretin (CL0287) T6_Ig_like; T6 antigen Ig like domain (PF18002; HMM-score: 16.9)
  LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 15.8)
  no clan defined TMCO5; TMCO5 family (PF14992; HMM-score: 15.6)
  DUF7449; Domain of unknown function (DUF7449) (PF24242; HMM-score: 14.1)
  OML_zippers (CL0590) LPP; Lipoprotein leucine-zipper (PF04728; HMM-score: 14)
  no clan defined DUF6317; Family of unknown function (DUF6317) (PF19840; HMM-score: 13.6)
  and 19 more
  DUF4763; Domain of unknown function (DUF4763) (PF15960; HMM-score: 13.5)
  YabA; Initiation control protein YabA (PF06156; HMM-score: 13.2)
  EsxAB (CL0352) Hydro_N_hd; Hydrolase N-terminal helical domain (PF22905; HMM-score: 13)
  DUF5344; Family of unknown function (DUF5344) (PF17279; HMM-score: 12.9)
  no clan defined DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 12.3)
  LppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 11.7)
  no clan defined MRPL52; Mitoribosomal protein mL52 (PF18699; HMM-score: 10.8)
  LemA; LemA family (PF04011; HMM-score: 10)
  PoNi_N; PoNe immunity protein (PoNi), N-terminal (PF08928; HMM-score: 9.6)
  Uso1_p115_C; Uso1 / p115 like vesicle tethering protein, C terminal region (PF04871; HMM-score: 9.2)
  FlaC_arch; Flagella accessory protein C (FlaC) (PF05377; HMM-score: 8.6)
  APG6_N; Apg6 coiled-coil region (PF17675; HMM-score: 8.3)
  Cnn_1N; Centrosomin N-terminal motif 1 (PF07989; HMM-score: 8.1)
  Ax_dynein_light; Axonemal dynein light chain (PF10211; HMM-score: 7.9)
  bZIP (CL0018) bZIP_1; bZIP transcription factor (PF00170; HMM-score: 7.4)
  MazG (CL0231) Nuf2_DHR10-like; Nuf2, DHR10-like domain (PF18595; HMM-score: 7.4)
  no clan defined GrpE; GrpE (PF01025; HMM-score: 7.3)
  RPW8; Arabidopsis broad-spectrum mildew resistance protein RPW8 (PF05659; HMM-score: 6.9)
  Prominin; Prominin (PF05478; HMM-score: 4.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0001
  • Cytoplasmic Membrane Score: 0.6328
  • Cell wall & surface Score: 0.0317
  • Extracellular Score: 0.3354
• LocateP:
• SignalP: Signal peptide LIPO(Sec/SPII) length 16 aa
  • SP(Sec/SPI): 0.000578
  • TAT(Tat/SPI): 0.000055
  • LIPO(Sec/SPII): 0.999143
  • Cleavage Site: CS pos: 16-17. LAG-CG. Pr: 0.9997
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446660340 NCBI
• RefSeq: WP_000737686 NCBI
• UniProt: see SAUSA300_1492

⊟Protein sequence

• MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEKDVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNHAFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications