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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS09250 [old locus tag: SAUSA300_1693 ]
- pan locus tag?: SAUPAN004414000
- symbol: SAUSA300_RS09250
- pan gene symbol?: —
- synonym:
- product: MBL fold metallo-hydrolase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS09250 [old locus tag: SAUSA300_1693 ]
- symbol: SAUSA300_RS09250
- product: MBL fold metallo-hydrolase
- replicon: chromosome
- strand: -
- coordinates: 1867001..1867843
- length: 843
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (1867001..1867843) NCBI
- BioCyc: SAUSA300_RS09250 BioCyc
- MicrobesOnline: see SAUSA300_1693
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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841ATGAAAATCGGGGATATATCTATTCATTATCTAAATGGTGGCAATACAAAAATGGATGGC
GGTGCAATGTTTGGTGTTGTTCCGAAGCCGTTGTGGTCAAAGCAATACAATGCAAATGAA
CGAAATCAAATCAATTTACCGACACATCCAATTTTGATTCAAACGGCTCAATATAATTTG
ATTATAGATGCAGGTATTGGTAATGGTAAATTATCTGAAAAGCAATTACGTAATTTTGGA
GTAGATGAAGAAAGTCATATAATTGCTGATTTGGCAAATTATAATTTAACGCCAAAGGAT
ATTGATTATGTGCTAATGACACATATGCATTTTGATCATGCTGCCGGTTTGACTGATCAA
GCAGGACATGCAATTTTTGAAAATGCGATTCATGTTGTGCAACAAGATGAGTGGCATGAG
TTTATTGCACCTAATATAAGAAGTAAATCAACTTACTGGGATAAGAATAAAGGCGATTAT
AGTAACAAGTTGATTTTATTCGAAAAACATTTTGAACCGGTTCTGGGTATCAAGATGCAA
CATAGTGGAGGTCATAGCTTTGGCCACACGATTATTACGATTGAAAGTCAAGGAGATAAA
GCAGTTCATATGGGTGATATATTCCCAACTACTGCACATAAAAATCCTCTATGGGTAACG
GCATATGATGATTATCCTATGCAATCGATTCGTGAAAAAGAACGCATGATACCATATTTT
ATTCAGCAACAATATTGGTTCTTGTTTTATCATGATGAAAACTACTTTGCTGTAAAATAC
AGCGATAATGGTGAAAACATAGATGCATATATTTTACGTGAAACATTAGTTGATAATAAC
TAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS09250 [old locus tag: SAUSA300_1693 ]
- symbol: SAUSA300_RS09250
- description: MBL fold metallo-hydrolase
- length: 280
- theoretical pI: 6.06469
- theoretical MW: 32189
- GRAVY: -0.491429
⊟Function[edit | edit source]
- TIGRFAM: Transcription RNA processing ribonuclease Z (TIGR02651; EC 3.1.26.11; HMM-score: 19.7)Unknown function Enzymes of unknown specificity quinoprotein relay system zinc metallohydrolase 2 (TIGR04559; EC 3.-.-.-; HMM-score: 17.1)and 2 morephosphonate metabolism protein PhnP (TIGR03307; HMM-score: 12.8)arCOG00543 universal archaeal KH-domain/beta-lactamase-domain protein (TIGR03675; HMM-score: 12.8)
- TheSEED: see SAUSA300_1693
- PFAM: Metallo-HOrase (CL0381) Lactamase_B; Metallo-beta-lactamase superfamily (PF00753; HMM-score: 55.1)and 2 moreLactamase_B_2; Beta-lactamase superfamily domain (PF12706; HMM-score: 22.2)Lactamase_B_3; Beta-lactamase superfamily domain (PF13483; HMM-score: 21.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.027381
- TAT(Tat/SPI): 0.000388
- LIPO(Sec/SPII): 0.00277
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446612234 NCBI
- RefSeq: WP_000689580 NCBI
- UniProt: see SAUSA300_1693
⊟Protein sequence[edit | edit source]
- MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQINLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDIDYVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDYSNKLILFEKHFEPVLGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLWVTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDNGENIDAYILRETLVDNN
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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