PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS11225 [old locus tag: SAUSA300_2040 ]
• pan locus tag^?: SAUPAN005357000
• symbol: SAUSA300_RS11225
• pan gene symbol^?: rodA
• synonym:
• product: cell division protein FtsW

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS11225 [old locus tag: SAUSA300_2040 ]
• symbol: SAUSA300_RS11225
• product: cell division protein FtsW
• replicon: chromosome
• strand: +
• coordinates: 2206043..2207245
• length: 1203
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2206043..2207245) NCBI
• BioCyc: SAUSA300_RS11225 BioCyc
• MicrobesOnline: see SAUSA300_2040

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  
  ATGAATTATTCATCTCGTCAACAGCCGGATAAGCATTGGCTTCGCAAAGTAGACTGGGTA
  TTAGTAGCCACTATAGCTGTTTTAGCAATTTTCAGTGTTCTGCTTATTAACTCGGCAATG
  GGCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGT
  GCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATAT
  TTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCT
  ATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTATCAGTATTCAG
  CCATCTGAATTCATGAAAATTATTTTAATTTTAGCATTAGCGCGTGTCGTTTCTAGACAT
  AATCAATTCACATTCAATAAATCATTCCAAAGTGATTTGTTATTATTTTTCAAAATTATT
  GGTGTCTCGTTAGTACCAAGTATTTTAATATTACTGCAAAATGACCTAGGAACTACATTA
  GTATTAGCTGCTATTATTGCAGGTGTGATGTTAGTAAGTGGTATAACATGGCGTATCTTA
  GCACCTATCTTTATTACAGGTATTGTTGGTGCAATGACAGTCATTTTAGGTATTCTATAT
  GCACCCGCATTAATTGAAAATTTATTAGGTGTCCAACTGTATCAAATGGGACGAATCAAT
  TCATGGCTTGACCCCTATACATATAGTAGTGGTGATGGCTATCATTTAACTGAATCACTT
  AAAGCTATCGGTTCTGGACAGTTACTAGGTAAAGGATACAATCACGGTGAAGTTTATATA
  CCTGAAAATCATACTGACTTTATCTTTTCAGTGATTGGAGAGGAACTTGGCTTTATCGGT
  TCTGTCATATTGATCTTAATATTTTTATTTTTAATCTTCCATCTAATAAGATTAGCTGCG
  AAAATTGAAGATCAATTTAACAAAATCTTTATCGTTGGTTTCGTCACTTTACTTGTGTTC
  CATATTTTACAAAATATTGGTATGACAATTCAGTTGTTACCAATCACTGGTATTCCATTA
  CCATTTATTAGTTATGGTGGTAGTGCGCTATGGAGTATGATGACTGGAATAGGTATAGTC
  TTATCAATTTATTATCATGAACCAAAACGATATGTCGATTTATACCATCCAAAAAGTAAT
  TAA
  60
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  420
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  840
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  960
  1020
  1080
  1140
  1200
  1203
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS11225 [old locus tag: SAUSA300_2040 ]
• symbol: SAUSA300_RS11225
• description: cell division protein FtsW
• length: 400
• theoretical pI: 9.31632
• theoretical MW: 44600.8
• GRAVY: 0.765

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan rod shape-determining protein RodA (TIGR02210; HMM-score: 326.2)
  Cellular processes Cellular processes Cell division rod shape-determining protein RodA (TIGR02210; HMM-score: 326.2)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan cell division protein FtsW (TIGR02614; HMM-score: 273.1)
  Cellular processes Cellular processes Cell division cell division protein FtsW (TIGR02614; HMM-score: 273.1)
  and 2 more
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan stage V sporulation protein E (TIGR02615; HMM-score: 199.3)
  Cellular processes Cellular processes Sporulation and germination stage V sporulation protein E (TIGR02615; HMM-score: 199.3)
• TheSEED: see SAUSA300_2040
• PFAM:
  Membrane_trans (CL0142) FTSW_RODA_SPOVE; Cell cycle protein (PF01098; HMM-score: 319.3)
  and 1 more
  no clan defined Clostridium_P47; Clostridium P-47 protein (PF06597; HMM-score: 11.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.022773
  • TAT(Tat/SPI): 0.000872
  • LIPO(Sec/SPII): 0.025839
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: 447032683 NCBI
• RefSeq: WP_001109939 NCBI
• UniProt: see SAUSA300_2040

⊟Protein sequence

• MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQYSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPITPIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKIIGVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGILYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHGEVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGFVTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYVDLYHPKSN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications