From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS11645 [old locus tag: SAUSA300_2114 ]
  • pan locus tag?: SAUPAN005509000
  • symbol: SAUSA300_RS11645
  • pan gene symbol?: rocF
  • synonym:
  • product: arginase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS11645 [old locus tag: SAUSA300_2114 ]
  • symbol: SAUSA300_RS11645
  • product: arginase
  • replicon: chromosome
  • strand: -
  • coordinates: 2289284..2290192
  • length: 909
  • essential: unknown

Accession numbers[edit | edit source]

  • Location: NC_007793 (2289284..2290192) NCBI
  • BioCyc:
  • MicrobesOnline:

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGACAAAGACAAAAGCAATTGATATTATAGGTGCACCATCAACATTTGGACAAAGAAAA
    TTAGGTGTTGATTTAGGACCAACAGCAATTAGATATGCTGGATTAATTTCAAGATTAAAG
    CAATTAGACCTTGATGTATATGACAAGGGGGATATTAAGGTACCTGCTGTGAACATTGAA
    AAATTTCATAGTGAACAAAAAGGATTAAGAAATTATGATGAAATTATAGATGTTAATCAA
    AAATTAAATAAAGAGGTTTCAGCATCAATTGAAAATAACAGATTTCCTCTAGTTCTTGGT
    GGAGATCATTCTATTGCGGTAGGTTCAGTATCAGCAATAAGTAAACATTATAATAATTTA
    GGTGTTATTTGGTATGATGCACATGGTGATTTAAATATACCTGAAGAGTCACCAAGTGGA
    AATATTCATGGTATGCCTCTAAGGATTTTGACAGGCGAAGGTCCCAAAGAACTTTTAGAA
    TTAAATAGTAATGTAATCAAGCCAGAAAACATCGTACTAATTGGTATGAGAGATTTAGAT
    AAAGGTGAAAGACAATTTATCAAAGATCATAATATTAAAACATTTACTATGTCAGATATT
    GATAAATTGGGGATAAAGGAAGTAATTGAAAATACAATAGAATATTTGAAGTCACGCAAT
    GTTGATGGCGTTCATTTATCTTTAGATGTTGATGCTTTAGATCCGCTTGAAACGCCAGGC
    ACTGGTACTAGAGTTTTGGGTGGTCTTAGTTATAGAGAAAGCCATTTTGCATTGGAATTA
    CTGCATCAATCACATTTAATTTCCTCAATGGATTTAGTTGAAGTAAATCCATTGATTGAC
    AGTAATAATCATACTGCTGAACAAGCGGTTTCATTAGTTGGAACATTTTTTGGTGAAACT
    TTATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    909

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS11645 [old locus tag: SAUSA300_2114 ]
  • symbol: SAUSA300_RS11645
  • description: arginase
  • length: 302
  • theoretical pI: 5.21395
  • theoretical MW: 33264.6
  • GRAVY: -0.216887

Function[edit | edit source]

  • reaction:
    EC 3.5.3.1?  ExPASy
    Arginase L-arginine + H2O = L-ornithine + urea
  • TIGRFAM:
    arginase (TIGR01229; EC 3.5.3.1; HMM-score: 329.6)
    and 2 more
    agmatinase (TIGR01230; EC 3.5.3.11; HMM-score: 108)
    Metabolism Energy metabolism Amino acids and amines formimidoylglutamase (TIGR01227; EC 3.5.3.8; HMM-score: 98.8)
  • TheSEED:
  • PFAM:
    Arginase (CL0302) Arginase; Arginase family (PF00491; HMM-score: 258.3)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mn2+
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003747
    • TAT(Tat/SPI): 0.000601
    • LIPO(Sec/SPII): 0.000629
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

  • GI: 446090012 NCBI
  • RefSeq: WP_000167867 NCBI
  • UniProt:

Protein sequence[edit | edit source]

  • MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDLDVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGDHSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLELNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKSRNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVNPLIDSNNHTAEQAVSLVGTFFGETLL

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]