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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS11715 [old locus tag: SAUSA300_2127 ]
  • pan locus tag?: SAUPAN005555000
  • symbol: SAUSA300_RS11715
  • pan gene symbol?: sepA
  • synonym:
  • product: multidrug resistance efflux pump SepA

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS11715 [old locus tag: SAUSA300_2127 ]
  • symbol: SAUSA300_RS11715
  • product: multidrug resistance efflux pump SepA
  • replicon: chromosome
  • strand: -
  • coordinates: 2301007..2301480
  • length: 474
  • essential: unknown

Accession numbers[edit | edit source]

  • Location: NC_007793 (2301007..2301480) NCBI
  • BioCyc:
  • MicrobesOnline:

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGATTGTGAATTATCTTAAACATAAGTTTTATAACTTGTTAACTACAATGATTGTTCTC
    TTTATTTTTGTACTTTCTGGTGCGATTTTTTTAACATTTCTAGGGTTTGGTTTATATGGA
    TTAAGTAGAATACTTATTTATTTTAGGTTGGGTGACTTTACATATAATAGAAGTATGTAC
    GATAACCTATTATATTATGGCAGTTATATAATCTTTGGGTACTTCATCATATTTGCAGTC
    GAGCATTTAATGGATTATTTTAGAAAAATGTTGCCTGAAAATGCATATTTTAGAGGCGCG
    ACTTTTCATTTGATTTCTTACACAGTTGCAACAACGTTATTTTATTTTATTATTCATCTG
    AACTATGTGTATATCAATATCGATTTTTGGGTCATCATGGTTATTATAGGGTTCTTATAT
    GTTTGTAAGCTTCAATTTTACCCTGAAAGTAAAAATTTAAATAATAGAAAATAG
    60
    120
    180
    240
    300
    360
    420
    474

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS11715 [old locus tag: SAUSA300_2127 ]
  • symbol: SAUSA300_RS11715
  • description: multidrug resistance efflux pump SepA
  • length: 157
  • theoretical pI: 9.40365
  • theoretical MW: 18899.3
  • GRAVY: 0.681529

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED:
  • PFAM:
    no clan defined SepA; Multidrug Resistance efflux pump (PF17080; HMM-score: 198)
    and 1 more
    DUF2070; Predicted membrane protein (DUF2070) (PF09843; HMM-score: 5)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002375
    • TAT(Tat/SPI): 0.000374
    • LIPO(Sec/SPII): 0.032554
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit | edit source]

  • GI: 446559511 NCBI
  • RefSeq: WP_000636857 NCBI
  • UniProt:

Protein sequence[edit | edit source]

  • MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRILIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATFHLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]