PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS12550 [old locus tag: SAUSA300_2270 ]
• pan locus tag^?: SAUPAN005803000
• symbol: SAUSA300_RS12550
• pan gene symbol^?: glvC
• synonym:
• product: PTS alpha-glucoside transporter subunit IIBC

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS12550 [old locus tag: SAUSA300_2270 ]
• symbol: SAUSA300_RS12550
• product: PTS alpha-glucoside transporter subunit IIBC
• replicon: chromosome
• strand: -
• coordinates: 2439955..2441559
• length: 1605
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2439955..2441559) NCBI
• BioCyc: SAUSA300_RS12550 BioCyc
• MicrobesOnline: see SAUSA300_2270

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAATGCGATAAAGCGTTTCGGTAGTGCAATGATTGTACCGGTATTAATGTTCGCTTTC
  TTTGGGATAGTGCTTGGCTTTGCGACATTATTTAAAAATCCTACAATCATGGGGAGTTTA
  GCAGATCAACATACGTTTTGGTTTAAATTTTGGTCAGTAATAGAATCTGGTGGCTGGGTT
  ATTTTTACGCATATGGAAGTTGTATTTGTTGTAGGTCTACCACTTTCTTTAGCTAAAAAA
  GCACCAGGTCATGCAGCACTTGCAGCATTGATGGGTTACTTAATGTTTAATACATTTATA
  AATGCCATTTTAACACAATGGCCACATACGTTTGGCGCTAATTTAGAAAAAGGCGTTGAA
  AATGTACCTGGTTTAAAATCAATTGCAGGTATTGCCACATTGGATACGAATATTCTAGGT
  GGTATTATTATTTCAGCTATTATTACTTGGATTCACAATAGATACTATAGTAAACGATTA
  CCAGAAATGGTTGGTGTATTCCAAGGTTTAACATTTGTAGTTACGATTTCATTTTTTGTG
  ATGTTACCATTAGCAGCAATTACTTGTGTTATTTGGCCAACTGTTCAGCACGGTATTGGT
  TCAATGCAACACTTTATCATTGCTTCAGGCTATATCGGTGTATGGTTGTATCACTTCTTA
  GAACGTGTATTAATACCAACAGGATTACATCACTTTATTTATGCACCAATTGAAGTTGGA
  CCAGTAGTAGTCAATCACGGCTTGAAAGCTGAATGGTTACAACATTTAAATGAGTTTGCT
  AAGAGTACTAAGCCATTAAAAGAGCAATTCCCTTATGGCTTTATGCTTCAAGGAAATGGG
  AAAGTATTTGGATGTTTAGGTATTGCTTTAGCAATGTATGCTACGACACCTAAAGAAAAT
  CGTAAAAAGGTGGCGGCACTACTTATTCCTGCCACATTAACAGCAGTCGTTGTAGGTATT
  ACTGAGCCATTAGAGTTTACATTCTTATTCATCGCACCATACTTATTTGTTTTACACGCA
  GTTTTAGCAGCATCAATGGATACTTTAATGTACGCATTTGGTGTTGTAGGTAACATGGGT
  GGTGGCTTGTTAGACTTTATTTCAACAAACTGGTTGCCATTAGGTAAAGAACATTGGGGT
  ACTTATGTTGCACAAGTTATTATCGGTCTGATATTTGTAGCGATTTACTTCTTCTTATTC
  AGATTCTTAATTTTGAAATTTGATATTCCATTACCTGGACGTAAGAAAACAGAAGAGGAA
  GTTAAATTATTTTCTAAGCAAGATTACAAAAATAAAAAAGGTGATAGCGTAGATTCTAAA
  CGTGCTTCATCTGGTAATGAATACGAAGACAAAGCTGCATATTACCTCGACGGTTTAGGC
  GGCAAAGAAAATATCAAAGATGTTACTAACTGTACAACAAGATTACGCTTAACTGTTTAT
  GATGAAAGTAAAGTTGCAGATACGGAATATTTCACGCATCAACAAATGGCACACGGATTA
  GTAAAGAGTGGTAAGAGTATCCAAGTTGTAGTAGGTATGACAGTACCACAAGTTCGTGAA
  GCATTTGAACAAATGGTTGAGGATCAATCGTCCGAAGATAAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS12550 [old locus tag: SAUSA300_2270 ]
• symbol: SAUSA300_RS12550
• description: PTS alpha-glucoside transporter subunit IIBC
• length: 534
• theoretical pI: 8.64496
• theoretical MW: 59329.2
• GRAVY: 0.366105

⊟Function

• reaction:
  EC 2.7.1.69?  ExPASy
  Transferred entry: 2.7.1.191, 2.7.1.192, 2.7.1.193, 2.7.1.194, 2.7.1.195, 2.7.1.196, 2.7.1.197, 2.7.1.198, 2.7.1.199, 2.7.1.200, 2.7.1.201, 2.7.1.202, 2.7.1.203, 2.7.1.204, 2.7.1.205, 2.7.1.206, 2.7.1.207 and 2.7.1.208
• TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 966.1)
  and 11 more
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 290.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 278.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 277.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 237.7)
  Signal transduction Signal transduction PTS PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 237.7)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 162)
  PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 57.8)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 56)
  Signal transduction Signal transduction PTS PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 56)
  PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 52.5)
  PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 49.5)
• TheSEED: see SAUSA300_2270
• PFAM:
  PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 221.7)
  and 2 more
  no clan defined PTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 53.9)
  Ldr_toxin; Toxin Ldr, type I toxin-antitoxin system (PF13940; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.065349
  • TAT(Tat/SPI): 0.00115
  • LIPO(Sec/SPII): 0.019006
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: 446914246 NCBI
• RefSeq: WP_000991502 NCBI
• UniProt: see SAUSA300_2270

⊟Protein sequence

• MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQHTFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINAILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRLPEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYHFLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFMLQGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPYLFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIFVAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASSGNEYEDKAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQVVVGMTVPQVREAFEQMVEDQSSEDK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA, GlvR* see SAUSA300_2270

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications