From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS12565 [old locus tag: SAUSA300_2273 ]
  • pan locus tag?: SAUPAN005807000
  • symbol: SAUSA300_RS12565
  • pan gene symbol?: —
  • synonym:
  • product: sodium:proton antiporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS12565 [old locus tag: SAUSA300_2273 ]
  • symbol: SAUSA300_RS12565
  • product: sodium:proton antiporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2443384..2444697
  • length: 1314
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGAAGTACATAAAAAGAGCAATGCTTGGGCATTATTCCCCTTGTTATTATTTGTGGCG
    TTGTTTTTAGGCGTAGGTATTATCACAGGTGATTTTACTTCAATGCCATTAAATGTTGCA
    ATTACGATAACGGTAATTGTGGCGTTATTAATGAATCGAAAAGAATCATTTGCTAAAAAA
    GTTGAGGTCTTCACGAAAGGTGCAGGCCATTCAAACATTGTTTTAATGATGTTGATTTTT
    ATTTTAGCAGGTGCATTTTCAAATACAGCTGAAAAAATGGGTGGCGTAAAGTCGACAGTT
    AATTTAGGACTATCATTAATCCCAGAAAATTTAATTATTGTAGGATTGTTTGTGATTTGT
    ATGTTTGTTTCTATATCTATGGGGACGTCTGTAGGAACTGTTGCTGCAATAGCACCTGTT
    GGTTACGGATTTGCACAAGCGACAGATGTACCAACTGCGCTAGCAATGGCTACAGTTGTT
    GGAGGTGCAATGTTCGGCGATAATCTATCAATGATTTCAGATACAACAATTGCTGCGGTT
    AGAACACAACATACGAAAATGAAAGATAAATTCAGAGTAAACTTTAAAATTGTATTGCCA
    GGTGCTATTTTAACGATTATTATTCTGTATTTCTTAACGAATGGCATTTCTTTAAATCAT
    GCTAAAAACTATGATTATAATCTAATTAAAGTTGTACCGTATATCTTAGTATTGGTATTA
    GCATTATTAGGTGTAAATGTCATTATCGTATTGATTGGCGGAACATTATTAGCCGGAATC
    ATTGGTCTCATAGATGGTTCATTCGGTTGGATGGGGCTACTAGATGCAGTGTCCAAAGGT
    ATTATAAGCATGGAAGATATTGCTATGATTGCGTTGCTAATTGGTGGTTTAGTCGGTATT
    ATTCAACACAATGGTGGTATTGAATGGTTGTTGCAATTTGTTCGCTCTAAAGTGAAATCA
    AAGCGTGGCGCAGAATTAGGAATTGCTAGTTTGGTAAGTGTCGCAGATATTGCCACGGCA
    AACAACACCATCTCTATTATTATGTCAGGTCCTTTAGCTAAAAATATTGCAGATGAATAT
    GATGTAGATTCAAGGAAATCAGCAAGTATTCTTGATATATTTGGAGGCTGCTTCCAAGGT
    TTATTACCATATAGCCCGCAAGTCATTTCAGCGGCTGGTGTAGCTGGTATTTCACCGTTA
    ATGTTATTCCCATACAGCATTTATCCTATATTATTAGGTGTATGCGGACTTATTGCGATT
    TTATTTAATTTTCCAAAGCTAAAAAAGAACACTAGTCAAGAAATGAAACATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1314

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS12565 [old locus tag: SAUSA300_2273 ]
  • symbol: SAUSA300_RS12565
  • description: sodium:proton antiporter
  • length: 437
  • theoretical pI: 9.40995
  • theoretical MW: 46324.1
  • GRAVY: 0.848513

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds Na+/H+ antiporter NhaC (TIGR00931; HMM-score: 86.9)
  • TheSEED: see SAUSA300_2273
  • PFAM:
    IT (CL0182) Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 79.1)
    and 4 more
    Na_H_antiport_2; Na+-H+ antiporter family (PF13726; HMM-score: 21.2)
    CitMHS; Citrate transporter (PF03600; HMM-score: 19.1)
    DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 14.5)
    GPCR_A (CL0192) Ceramidase; Ceramidase (PF05875; HMM-score: 5.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.099348
    • TAT(Tat/SPI): 0.000884
    • LIPO(Sec/SPII): 0.067644
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITITVIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNLGLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVVGGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISLNHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDAVSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSVADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAAGVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_RS12565&oldid=82842"