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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

organism: Staphylococcus aureus USA300_FPR3757
locus tag: SAUSA300_RS13235 [old locus tag: SAUSA300_2391 ]
pan locus tag^?: SAUPAN005998000
symbol: SAUSA300_RS13235
pan gene symbol^?: opuCC
synonym:
product: amino acid transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SAUSA300_RS13235 [old locus tag: SAUSA300_2391 ]
symbol: SAUSA300_RS13235
product: amino acid transporter
replicon: chromosome
strand: -
coordinates: 2574313..2575254
length: 942
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Location: NC_007793 (2574313..2575254) NCBI
BioCyc: SAUSA300_RS13235 BioCyc
MicrobesOnline: see SAUSA300_2391

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
421
481
541
601
661
721
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841
901
ATGAAGAAAATTAAATATATACTTGTCGTGTTTGTCTTATCGCTTACCGTATTATCTGGA
TGTAGTTTGCCCGGACTAGGTAGTAAGAGCACGAAAAATGATGTCAAAATTACAGCATTA
TCAACAAGCGAATCGCAAATTATTTCACATATGTTACGGTTGTTAATAGAGCATGATACA
CACGGTAAGATAAAGCCAACATTAGTAAATAATTTAGGGTCAAGTACGATTCAACATAAT
GCCTTAATTAATGGGGATGCTAATATATCAGGTGTTAGATATAATGGCACAGATTTAACG
GGAGCTTTGAAGGAAGCACCAATTAAAAATCCTAAGAAAGCAATGATAGCAACACAACAA
GGATTTAAAAAGAAATTTGATCAAACGTTTTTTGATTCGTATGGTTTTGCGAATACGTAT
GCATTCATGGTAACGAAGGAAACCGCTAAAAAATATCATTTAGAGACAGTTTCAGATTTA
GCAAAGCATAGTAAAGATTTACGTTTAGGTATGGATAGTTCATGGATGAATCGTAAAGGC
GATGGCTATGAAGGATTTAAAAAAGAGTATGGTTTTGACTTTGGTACAGTGAGACCAATG
CAAATAGGTCTAGTCTACGACGCATTAAACTCAGAGAAGTTAGACGTTGCATTAGGTTAT
TCTACAGATGGTCGAATTGCGGCGTATGATTTGAAAGTACTTAAAGATGATAAACAATTT
TTCCCACCTTATGCTGCGAGTGCTGTTGCAACAAATGAATTATTACGGCAACACCCAGAA
CTTAAAACGACGATTAATAAGTTGACAGGAAAGATTTCGACTTCAGAGATGCAACGCTTG
AATTATGAAGCGGATGGTAAAGGTAAAGAACCTGCTGTCGTCGCAGAAGAATTTTTGAAA
AAACACCACTATTTTGATAAACAGAAAGGTGGTCATAAGTAA
60
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942

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SAUSA300_RS13235 [old locus tag: SAUSA300_2391 ]
symbol: SAUSA300_RS13235
description: amino acid transporter
length: 313
theoretical pI: 9.89386
theoretical MW: 34812.7
GRAVY: -0.4377

⊟Function[edit | edit source]

reaction:
EC 3.6.3.32? ExPASy
Quaternary-amine-transporting ATPase ATP + H₂O + quaternary amine(Out) = ADP + phosphate + quaternary amine(In)
TIGRFAM:
Signal transduction Regulatory functions Protein interactions Phr family secreted Rap phosphatase inhibitor (TIGR04429; HMM-score: 14.7)
TheSEED: see SAUSA300_2391
PFAM:
PBP (CL0177) OpuAC; Substrate binding domain of ABC-type glycine betaine transport system (PF04069; HMM-score: 180)
and 2 more
LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 12.9)
no clan defined Nrap_D2; Nrap protein PAP/OAS-like domain (PF17403; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 9.68
- Cellwall Score: 0.17
- Extracellular Score: 0.16
- Internal Helix: 1
LocateP:
SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
- SP(Sec/SPI): 0.001304
- TAT(Tat/SPI): 0.000196
- LIPO(Sec/SPII): 0.998173
- Cleavage Site: CS pos: 20-21. LSG-CS. Pr: 0.9989
predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

GI: 446644207 NCBI
RefSeq: WP_000721553 NCBI
UniProt: see SAUSA300_2391

⊟Protein sequence[edit | edit source]

MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSESQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGALKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDLAKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVALGYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSEMQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

regulator: CodY see SAUSA300_2391

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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