PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS13525 [old locus tag: SAUSA300_2440 ]
• pan locus tag^?: SAUPAN006097000
• symbol: SAUSA300_RS13525
• pan gene symbol^?: fnbB
• synonym:
• product: fibronectin-binding protein A

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS13525 [old locus tag: SAUSA300_2440 ]
• symbol: SAUSA300_RS13525
• product: fibronectin-binding protein A
• replicon: chromosome
• strand: -
• coordinates: 2629241..2632063
• length: 2823
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2629241..2632063) NCBI
• BioCyc: SAUSA300_RS13525 BioCyc
• MicrobesOnline: see SAUSA300_2440

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  1981
  2041
  2101
  2161
  2221
  2281
  2341
  2401
  2461
  2521
  2581
  2641
  2701
  2761
  2821
  
  GTGAAAAGCAATCTTAGATACGGCATAAGAAAACACAAATTGGGAGCGGCCTCAGTATTC
  TTAGGAACAATGATCGTTGTTGGAATGGGACAAGAAAAAGAAGCTGCAGCATCGGAACAA
  AACAATACTACAGTAGAGGAAAGTGGGAGTTCAGCTACTGAAAGTAAAGCAAGCGAAACA
  CAAACAACTACAAATAACGTTAATACAATAGATGAAACACAATCATACAGCGCGACATCA
  ACTGAGCAACCATCACAATCAACACAAGTAACAACAGAAGAAGCACCGAAAACTGTGCAA
  GCACCAAAAGTAGAAACTTCGCGAGTTGATTTGCCATCGGAAAAAGTTGCTGATAAGGAA
  ACTACAGGAACTCAAGTTGACATAGCTCAACCAAGTAACGTCTCAGAAATTAAACCAAGA
  ATGAAAAGATCAACTGACGTTACAGCAGTTGCAGAGAAAGAAGTAGTGGAAGAAACTAAA
  GCGACAGGTACAGATGTAACAAATAAAGTGGAAGTAGAAGAAGGTAGTGAAATTGTAGGA
  CATAAACAAGATACGAATGTTGTAAATCCTCATAACGCAGAAAGAGTAACCTTGAAATAT
  AAATGGAAATTTGGAGAAGGAATTAAGGCGGGAGATTATTTTGATTTCACATTAAGCGAT
  AATGTTGAAACTCATGGTATCTCAACACTGCGTAAAGTTCCGGAGATAAAAAGTACAGAT
  GGTCAAGTTATGGCGACAGGAGAAATAATTGGAGAAAGAAAAGTTAGATATACGTTTAAA
  GAATATGTACAAGAAAAGAAAGATTTAACTGCTGAATTATCTTTAAATCTATTTATTGAT
  CCTACAACAGTGACGCAAAAAGGTAACCAAAATGTTGAAGTTAAATTGGGTGAGACTACG
  GTTAGCAAAATATTTAATATTCAATATTTAGGTGGAGTTAGAGATAATTGGGGAGTAACA
  GCTAATGGTCGAATTGATACTTTAAATAAAGTAGATGGGAAATTTAGTCATTTTGCGTAC
  ATGAAACCTAACAACCAGTCGTTAAGCTCTGTGACAGTAACTGGTCAAGTAACTAAAGGA
  AATAAACCAGGGGTTAATAATCCAACAGTTAAGGTATATAAACACATTGGTTCAGACGAT
  TTAGCTGAAAGCGTATATGCAAAGCTTGATGATGTCAGCAAATTTGAAGATGTGACTGAT
  AATATGAGTTTAGATTTTGATACTAATGGTGGTTATTCTTTAAACTTTAATAATTTAGAC
  CAAAGTAAAAATTATGTAATAAAATATGAAGGGTATTATGATTCAAATGCTAGCAACTTA
  GAATTTCAAACACACCTTTTTGGATATTATAACTATTATTATACAAGTAATTTAACTTGG
  AAAAATGGCGTTGCATTTTACTCTAATAACGCTCAAGGCGACGGCAAAGATAAACTAAAG
  GAACCTATTATAGAACATAGTACTCCTATCGAACTTGAATTTAAATCAGAGCCGCCAGTG
  GAGAAGCATGAATTGACTGGTACAATCGAAGAAAGTAATGATTCTAAGCCAATTGATTTT
  GAATATCATACAGCTGTTGAAGGTGCAGAAGGTCATGCAGAAGGTACCATTGAAACTGAA
  GAAGATTCTATTCATGTAGACTTTGAAGAATCGACACATGAAAATTCAAAACATCATGCT
  GATGTTGTTGAATATGAAGAAGATACAAACCCAGGTGGTGGTCAGGTTACTACTGAGTCT
  AACCTAGTTGAATTTGACGAAGATTCTACAAAAGGTATTGTAACTGGTGCTGTTAGCGAT
  CATACAACAATTGAAGATACGAAAGAATATACGACTGAAAGTAATCTGATTGAACTAGTA
  GATGAACTACCTGAAGAACATGGTCAAGCGCAAGGACCAATCGAGGAAATTACTGAAAAC
  AATCATCATATTTCTCATTCTGGTTTAGGAACTGAAAATGGTCACGGTAATTATGGCGTG
  ATTGAAGAAATCGAAGAAAATAGCCACGTGGATATTAAGAGTGAATTAGGTTACGAAGGT
  GGCCAAAATAGCGGTAATCAGTCATTTGAGGAAGACACAGAAGAAGATAAACCGAAATAT
  GAACAAGGTGGCAATATCGTAGATATCGATTTCGATAGTGTACCTCAAATTCATGGTCAA
  AATAATGGTAACCAATCATTCGAAGAAGATACAGAGAAAGACAAACCTAAGTATGAACAA
  GGTGGTAATATCATTGATATCGACTTCGACAGTGTGCCACATATTCACGGATTCAATAAG
  CACACTGAAATTATTGAAGAAGATACAAATAAAGATAAACCAAATTATCAATTCGGTGGA
  CACAATAGTGTTGACTTTGAAGAAGATACACTTCCACAAGTAAGTGGTCATAATGAAGGT
  CAACAAACGATTGAAGAAGATACAACACCTCCAATCGTGCCACCAACGCCACCGACACCA
  GAAGTACCAAGCGAGCCGGAAACACCAACACCACCGACACCAGAAGTACCAAGCGAGCCG
  GAAACACCAACACCGCCAACGCCAGAGGTACCAACTGAACCTGGTAAACCAATACCACCT
  GCTAAAGAAGAACCTAAAAAACCTTCTAAACCAGTGGAACAAGGTAAAGTAGTAACACCT
  GTTATTGAAATCAATGAAAAGGTTAAAGCAGTGGTACCAACTAAAAAAGCACAATCTAAG
  AAATCTGAACTACCTGAAACAGGTGGAGAAGAATCAACAAACAACGGCATGTTGTTCGGC
  GGATTATTTAGCATTTTAGGTTTAGCGTTATTACGCAGAAATAAAAAGAATCACAAAGCA
  TAA
  60
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  2160
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  2520
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  2640
  2700
  2760
  2820
  2823
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS13525 [old locus tag: SAUSA300_2440 ]
• symbol: SAUSA300_RS13525
• description: fibronectin-binding protein A
• length: 940
• theoretical pI: 4.36624
• theoretical MW: 103554
• GRAVY: -0.87117

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 26.7)
  and 1 more
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 20.8)
• TheSEED: see SAUSA300_2440
• PFAM:
  Adhesin (CL0204) SdrG_C_C; C-terminus of bacterial fibrinogen-binding adhesin (PF10425; HMM-score: 133.5)
  no clan defined Fn_bind; Fibronectin binding repeat (PF02986; HMM-score: 107.5)
  and 2 more
  Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 34.5)
  YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 32)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 10
  • Extracellular Score: 0
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 37 aa
  • SP(Sec/SPI): 0.879583
  • TAT(Tat/SPI): 0.010762
  • LIPO(Sec/SPII): 0.095299
  • Cleavage Site: CS pos: 37-38. AAA-SE. Pr: 0.7097
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446764166 NCBI
• RefSeq: WP_000841422 NCBI
• UniProt: see SAUSA300_2440

⊟Protein sequence

• MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTTVEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPKTVQAPKVETSRVDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSTDVTAVAEKEVVEETKATGTDVTNKVEVEEGSEIVGHKQDTNVVNPHNAERVTLKYKWKFGEGIKAGDYFDFTLSDNVETHGISTLRKVPEIKSTDGQVMATGEIIGERKVRYTFKEYVQEKKDLTAELSLNLFIDPTTVTQKGNQNVEVKLGETTVSKIFNIQYLGGVRDNWGVTANGRIDTLNKVDGKFSHFAYMKPNNQSLSSVTVTGQVTKGNKPGVNNPTVKVYKHIGSDDLAESVYAKLDDVSKFEDVTDNMSLDFDTNGGYSLNFNNLDQSKNYVIKYEGYYDSNASNLEFQTHLFGYYNYYYTSNLTWKNGVAFYSNNAQGDGKDKLKEPIIEHSTPIELEFKSEPPVEKHELTGTIEESNDSKPIDFEYHTAVEGAEGHAEGTIETEEDSIHVDFEESTHENSKHHADVVEYEEDTNPGGGQVTTESNLVEFDEDSTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSFEEDTEKDKPKYEQGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPQVSGHNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPTEPGKPIPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVVPTKKAQSKKSELPETGGEESTNNGMLFGGLFSILGLALLRRNKKNHKA

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications