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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS14015 [old locus tag: SAUSA300_2526 ]
- pan locus tag?: SAUPAN006269000
- symbol: SAUSA300_RS14015
- pan gene symbol?: pyrD
- synonym:
- product: dihydroorotate dehydrogenase (quinone)
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS14015 [old locus tag: SAUSA300_2526 ]
- symbol: SAUSA300_RS14015
- product: dihydroorotate dehydrogenase (quinone)
- replicon: chromosome
- strand: +
- coordinates: 2727853..2728917
- length: 1065
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (2727853..2728917) NCBI
- BioCyc: SAUSA300_RS14015 BioCyc
- MicrobesOnline: see SAUSA300_2526
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1021ATGTACAAATTAATTAAACCTTTCTTATTCAAAATCGAACCCGAAAAAGCACACGGACTA
ACTATCGATGCATTAAAAACGTTACAAAAGTTTCCGGTTTTATTCCCAGTCGTCGATAAA
CTATTTACTTATAAGAATCCAACGTTATCACAAACGATACAAGGTAATACGTACGACAAT
CCAATTGGCTTAGCAGCTGGTTTCGACAAATCTTGCGAAGTACCAAAAGCATTGGAACAC
CTTGGATTCGGTGCTTTAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCT
CAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAAT
AATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTT
GGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATA
AAGGTTATTGATACGTTTAAACACGACGTTTCATTTTTCACAGTCAACATCAGTTCTCCA
AATACTGAAAATCTTCAAAACTTCCATGATAAAGATGAATTTTCAATGTTATGCCAAGCT
TTAACAGCATTTAAAAAACAACATGATGTAACAGTGCCAATTTATTTAAAACTAACGTCT
GATATGGATTTCGATGGCTTAAAAGCACTATTACCAGCGATTACTGAGACATTTGACGGT
ATCATCTTAGCAAACACAACGCGACAACGAGATGGTTTAACTTCTGCTAATAAAGTCGAA
GAAGGCGGTTTGAGTGGTCGTCCATTATTTGAACGTAATTTAAAATTGATTAAGTATGCT
TATCAGCAAACAAATGGTGAATTTTTAATTATAGGTACAGGCGGCGTATTCAGTACTGAA
GATGCAATCAAAATGATGCGTCACGGTGCGTCACTTATTCAAATTTATTCATCACTTGTT
ATTGAAGGCCCAGGTTTAACTAAGAAAATGAACAAAGGCATCGCACGTTACTTAAAAGAT
CATCATTTTGACAATGTCAGTGATATTATAGGACTAGATGCCTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS14015 [old locus tag: SAUSA300_2526 ]
- symbol: SAUSA300_RS14015
- description: dihydroorotate dehydrogenase (quinone)
- length: 354
- theoretical pI: 8.56958
- theoretical MW: 39536.3
- GRAVY: -0.222599
⊟Function[edit | edit source]
- reaction: EC 1.3.5.2? ExPASyDihydroorotate dehydrogenase (quinone) (S)-dihydroorotate + a quinone = orotate + a quinolEC 1.3.98.1? ExPASyDihydroorotate oxidase (fumarate) (S)-dihydroorotate + fumarate = orotate + succinate
- TIGRFAM: Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis dihydroorotate dehydrogenase (fumarate) (TIGR01036; EC 1.3.98.1; HMM-score: 328.6)and 2 moredihydroorotate dehydrogenase family protein (TIGR01037; EC 1.3.-.-; HMM-score: 100.4)Biosynthesis of cofactors, prosthetic groups, and carriers Other isopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 11)
- TheSEED: see SAUSA300_2526
- PFAM: TIM_barrel (CL0036) DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 244.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: FMN
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 1.05
- Cytoplasmic Membrane Score: 8.78
- Cellwall Score: 0.08
- Extracellular Score: 0.09
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004344
- TAT(Tat/SPI): 0.00029
- LIPO(Sec/SPII): 0.000607
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446199560 NCBI
- RefSeq: WP_000277415 NCBI
- UniProt: see SAUSA300_2526
⊟Protein sequence[edit | edit source]
- MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTYKNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQPRMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYIKVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTAFKKQHDVTVPIYLKLTSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKLIKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGIARYLKDHHFDNVSDIIGLDA
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAUSA300_RS11505 (deoA) pyrimidine-nucleoside phosphorylase [1] (data from MRSA252) SAUSA300_RS02805 50S ribosomal protein L10 [1] (data from MRSA252) SAUSA300_RS02850 elongation factor Tu [1] (data from MRSA252) SAUSA300_RS05365 dihydrolipoyl dehydrogenase [1] (data from MRSA252) SAUSA300_RS07100 dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex [1] (data from MRSA252) SAUSA300_RS07430 DNA-binding protein HU [1] (data from MRSA252) SAUSA300_RS08910 aldehyde dehydrogenase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)