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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2606 [new locus tag: SACOL_RS13635 ]
  • pan locus tag?: SAUPAN006269000
  • symbol: pyrD
  • pan gene symbol?: pyrD
  • synonym:
  • product: dihydroorotate dehydrogenase 2

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2606 [new locus tag: SACOL_RS13635 ]
  • symbol: pyrD
  • product: dihydroorotate dehydrogenase 2
  • replicon: chromosome
  • strand: +
  • coordinates: 2664549..2665613
  • length: 1065
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTACAAATTAATTAAACCTTTCTTATTCAAAATCGAACCCGAAAAAGCACACGGACTA
    ACTATCGATGCATTAAAAACGTTACAAAAGTTTCCGGTTTTATTCCCAGTCGTCGATAAA
    CTATTTACTTATAAGAATCCAACGTTATCACAAACGATACAAGGTAATACGTACGACAAT
    CCAATTGGCTTAGCAGCTGGTTTCGACAAATCTTGCGAAGTACCAAAAGCATTGGAACAC
    CTTGGATTCGGTGCTTTAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCT
    CAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAAT
    AATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTT
    GGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATA
    AAGGTTATTGATACGTTTAAACACGACGTTTCATTTTTCACAGTCAACATCAGTTCTCCA
    AATACTGAAAATCTTCAAAACTTCCATGATAAAGATGAATTTTCAATGTTATGCCAAGCT
    TTAACAGCATTTAAAAAACAACATGATGTAACAGTGCCAATTTATTTAAAACTAACGTCT
    GATATGGATTTCGATGGCTTAAAAGCACTATTACCAGCGATTACTGAGACATTTGACGGT
    ATCATCTTAGCAAACACAACGCGACAACGAGATGGTTTAACTTCTGCTAATAAAGTCGAA
    GAAGGCGGTTTGAGTGGTCGTCCATTATTTGAACGTAATTTAAAATTGATTAAGTATGCT
    TATCAGCAAACAAATGGTGAATTTTTAATTATAGGTACAGGCGGCGTATTCAGTACTGAA
    GATGCAATCAAAATGATGCGTCACGGTGCGTCACTTATTCAAATTTATTCATCACTTGTT
    ATTGAAGGCCCAGGTTTAACTAAGAAAATGAACAAAGGCATCGCACGTTACTTAAAAGAT
    CATCATTTTGACAATGTCAGTGATATTATAGGACTAGATGCCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1065

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2606 [new locus tag: SACOL_RS13635 ]
  • symbol: PyrD
  • description: dihydroorotate dehydrogenase 2
  • length: 354
  • theoretical pI: 8.56958
  • theoretical MW: 39536.3
  • GRAVY: -0.222599

⊟Function[edit | edit source]

  • reaction:
    EC 1.3.5.2?  ExPASy
    Dihydroorotate dehydrogenase (quinone) (S)-dihydroorotate + a quinone = orotate + a quinol
    EC 1.3.98.1?  ExPASy
    Dihydroorotate oxidase (fumarate) (S)-dihydroorotate + fumarate = orotate + succinate
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis dihydroorotate dehydrogenase (fumarate) (TIGR01036; EC 1.3.98.1; HMM-score: 328.6)
    and 2 more
    dihydroorotate dehydrogenase family protein (TIGR01037; EC 1.3.-.-; HMM-score: 100.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other isopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 11)
  • TheSEED  :
    • Dihydroorotate dehydrogenase (EC 1.3.3.1)
    Nucleosides and Nucleotides Pyrimidines De Novo Pyrimidine Synthesis  Dihydroorotate dehydrogenase (EC 1.3.3.1)
  • PFAM:
    TIM_barrel (CL0036) DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 244.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: FMN
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004344
    • TAT(Tat/SPI): 0.00029
    • LIPO(Sec/SPII): 0.000607
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTYKNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQPRMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYIKVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTAFKKQHDVTVPIYLKLTSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKLIKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGIARYLKDHHFDNVSDIIGLDA

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 98 [4]
  • interaction partners:
    SACOL1734(gapA2)glyceraldehyde 3-phosphate dehydrogenase 2  [5] (data from MRSA252)
    SACOL1513(hup)DNA-binding protein HU  [5] (data from MRSA252)
    SACOL1105(pdhD)dihydrolipoamide dehydrogenase  [5] (data from MRSA252)
    SACOL2128(pdp)pyrimidine-nucleoside phosphorylase  [5] (data from MRSA252)
    SACOL0585(rplJ)50S ribosomal protein L10  [5] (data from MRSA252)
    SACOL1448(sucB)dihydrolipoamide succinyltransferase  [5] (data from MRSA252)
    SACOL0594(tuf)elongation factor Tu  [5] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. ↑ 5.0 5.1 5.2 5.3 5.4 5.5 5.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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