PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS14490 [old locus tag: SAUSA300_2606 ]
• pan locus tag^?: SAUPAN006428000
• symbol: SAUSA300_RS14490
• pan gene symbol^?: hisF
• synonym:
• product: imidazole glycerol phosphate synthase cyclase subunit

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS14490 [old locus tag: SAUSA300_2606 ]
• symbol: SAUSA300_RS14490
• product: imidazole glycerol phosphate synthase cyclase subunit
• replicon: chromosome
• strand: -
• coordinates: 2834237..2834995
• length: 759
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2834237..2834995) NCBI
• BioCyc: SAUSA300_RS14490 BioCyc
• MicrobesOnline: see SAUSA300_2606

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATTAAAAAACGTATCATTCCATGTTTAGATGTCAAAGATGGTCGTGTCGTTAAAGGG
  ATTCAATTTAAAGGATTAAGGGATATTGGGAATCCTGTTGATTTAGCAATGTATTACAAT
  GAAGCGGGTGCTGATGAATTAGTATTTTTAGACATCTCTAAGACGGAAGAGGGTCATAGC
  TTAATGCTAGAAGTGATTGAACAGACAGCGTCACGCTTGTTTATCCCTCTTACTGTAGGG
  GGTGGGATTCAAAGTCTCGATGATATTACCCAATTGCTAAATCATGGTGCAGATAAAGTA
  TCATTAAATTCAAGTGCTTTAAAAAATCCACAGCTCATTAAACAAGCGAGTGATAAATTC
  GGTAGACAATGCATCTGCATAGCAATTGATAGCTATTATGATCCTGAAAGAAAAGCACAT
  TATTGTTGTACGACTGGTGGTAAAAAAATGACAAATATTAAAGTATATGACTGGGTACAG
  CAAGTAGAACAGTTAGGTGCAGGTGAGCTCCTCGTTACAAGTATGGGACATGATGGTATG
  AAACAAGGCTTTGATATTGAACACCTAGCAAATATTAAGTCTCTTGTAAATATTCCAATC
  ATTGCTTCTGGTGGTGGTGGCAATGCACAACACTTTGTAGAATTATTTGATCAGACGGAT
  GTTTCTGCAGGTTTAGCTGCAAGTATATTACATGATCGAGAAACGACGGTTCAATCTATT
  AAAGAAGTGATACGGCAAGGGGGTATAGCAGTAAGATGA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS14490 [old locus tag: SAUSA300_2606 ]
• symbol: SAUSA300_RS14490
• description: imidazole glycerol phosphate synthase cyclase subunit
• length: 252
• theoretical pI: 6.15747
• theoretical MW: 27498.4
• GRAVY: -0.097619

⊟Function

• reaction:
  5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H₂O?
• TIGRFAM:
  Metabolism Amino acid biosynthesis Histidine family imidazoleglycerol phosphate synthase, cyclase subunit (TIGR00735; HMM-score: 334.3)
  glycosyl amidation-associated protein WbuZ (TIGR03572; HMM-score: 271.3)
  and 12 more
  Metabolism Amino acid biosynthesis Histidine family 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase (TIGR00007; EC 5.3.1.16; HMM-score: 118.7)
  bifunctional HisA/TrpF protein (TIGR01919; EC 5.3.1.16,5.3.1.24; HMM-score: 74.2)
  Unknown function General hisA/hisF family protein (TIGR00734; HMM-score: 34.6)
  Metabolism Amino acid biosynthesis Histidine family phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (TIGR02129; EC 5.3.1.16; HMM-score: 33.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 24.3)
  Unknown function General putative TIM-barrel protein, nifR3 family (TIGR00737; HMM-score: 18.7)
  geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 16.2)
  dihydroorotate dehydrogenase family protein (TIGR01037; EC 1.3.-.-; HMM-score: 14.3)
  Metabolism Energy metabolism Glycolysis/gluconeogenesis fructose-1,6-bisphosphate aldolase, class II (TIGR01859; EC 4.1.2.13; HMM-score: 14.2)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA dihydrouridine synthase A (TIGR00742; EC 1.-.-.-; HMM-score: 13.6)
  mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 12.9)
  phosphoglycerol geranylgeranyltransferase (TIGR01769; EC 2.5.1.41; HMM-score: 12.3)
• TheSEED: see SAUSA300_2606
• PFAM:
  TIM_barrel (CL0036) His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 266.3)
  and 7 more
  Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 29.3)
  TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 26.6)
  NMO; Nitronate monooxygenase (PF03060; HMM-score: 21.7)
  G3P_antiterm; Glycerol-3-phosphate responsive antiterminator (PF04309; HMM-score: 20)
  PK_TIM (CL0151) PEP_mutase; Phosphoenolpyruvate phosphomutase (PF13714; HMM-score: 13.8)
  TIM_barrel (CL0036) ThiG; Thiazole biosynthesis protein ThiG (PF05690; HMM-score: 13)
  DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003209
  • TAT(Tat/SPI): 0.000081
  • LIPO(Sec/SPII): 0.000378
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446515263 NCBI
• RefSeq: WP_000592609 NCBI
• UniProt: see SAUSA300_2606

⊟Protein sequence

• MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGADELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQQVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQTDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR

⊟Experimental data

• experimentally validated:
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY, HisR* see SAUSA300_2606

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications