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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_03008
  • pan locus tag?: SAUPAN006428000
  • symbol: SAOUHSC_03008
  • pan gene symbol?: hisF
  • synonym:
  • product: multifunctional imidazole glycerol phosphate synthase subunit/phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_03008
  • symbol: SAOUHSC_03008
  • product: multifunctional imidazole glycerol phosphate synthase subunit/phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase
  • replicon: chromosome
  • strand: -
  • coordinates: 2782800..2783559
  • length: 759
  • essential: no DEG other strains
  • comment: The sequence of SAOUHSC_03008 was corrected based on the resequencing performed by Berscheid et al., 2012 [1], resulting in a shorter CDS.

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGATTAAAAAACGTATCATTCCATGTTTAGATGTCAAAGATGGTCGTGTCGTTAAAGGG
    ATTCAATTTAAAGGATTAAGGGATATTGGGAATCCTGTTGATTTAGCAATGTATTACAAT
    GAAGCGGGTGCTGATGAATTAGTATTTTTAGACATCTCTAAGACGGAAGAGGGTCATAGC
    TTAATGCTAGAAGTGATTGAACAGACAGCGTCACGCTTGTTTATCCCTCTTACTGTAGGG
    GGTGGGATTCAAAGTCTCGATGATATTACCCAATTGCTAAATCATGGTGCAGATAAAGTA
    TCATTAAATTCAAGTGCTTTAAAAAATCCACAGCTCATTAAACAAGCGAGTGATAAATTC
    GGTAGACAATGCATCTGCATAGCAATTGATAGCTATTATGATCCTGAAAGAAAAGCACAT
    TATTGTTGTACGACTGGTGGTAAAAAAATGACAAATATTAAAGTATATGACTGGGTACAG
    CAAGTAGAACAGTTAGGTGCAGGTGAGCTCCTCGTTACAAGTATGGGACATGATGGTATG
    AAACAAGGCTTTGATATTGAACACCTAGCAAATATTAAGTCTCTTGTAAATATTCCAATC
    ATTGCTTCTGGTGGTGGTGGCAATGCACAACACTTTGTAGAATTATTTGATCAGACGGAT
    GTTTCTGCAGGTTTAGCTGCAAGTATATTACATGATCGAGAAACGACGGTTCAATCTATT
    AAAGAAGTGATACGGCAAGGGGGTATAGCAGTAAGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    759

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_03008
  • symbol: SAOUHSC_03008
  • description: multifunctional imidazole glycerol phosphate synthase subunit/phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase
  • length: 252
  • theoretical pI: 6.15747
  • theoretical MW: 27498.4
  • GRAVY: -0.097619

Function[edit | edit source]

  • reaction:
    5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O?
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family imidazoleglycerol phosphate synthase, cyclase subunit (TIGR00735; HMM-score: 334.3)
    glycosyl amidation-associated protein WbuZ (TIGR03572; HMM-score: 271.3)
    and 12 more
    Metabolism Amino acid biosynthesis Histidine family 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase (TIGR00007; EC 5.3.1.16; HMM-score: 118.7)
    bifunctional HisA/TrpF protein (TIGR01919; EC 5.3.1.16,5.3.1.24; HMM-score: 74.2)
    Unknown function General hisA/hisF family protein (TIGR00734; HMM-score: 34.6)
    Metabolism Amino acid biosynthesis Histidine family phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (TIGR02129; EC 5.3.1.16; HMM-score: 33.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 24.3)
    Unknown function General putative TIM-barrel protein, nifR3 family (TIGR00737; HMM-score: 18.7)
    geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 16.2)
    dihydroorotate dehydrogenase family protein (TIGR01037; EC 1.3.-.-; HMM-score: 14.3)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis fructose-1,6-bisphosphate aldolase, class II (TIGR01859; EC 4.1.2.13; HMM-score: 14.2)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA dihydrouridine synthase A (TIGR00742; EC 1.-.-.-; HMM-score: 13.6)
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 12.9)
    phosphoglycerol geranylgeranyltransferase (TIGR01769; EC 2.5.1.41; HMM-score: 12.3)
  • TheSEED:  
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-)
    and 5 more
    Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)
    Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)
    Histidine Biosynthesis in plants  Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-)
    Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)
    Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)
  • PFAM:
    TIM_barrel (CL0036) His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 266.3)
    and 7 more
    Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 29.3)
    TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 26.6)
    NMO; Nitronate monooxygenase (PF03060; HMM-score: 21.7)
    G3P_antiterm; Glycerol-3-phosphate responsive antiterminator (PF04309; HMM-score: 20)
    PK_TIM (CL0151) PEP_mutase; Phosphoenolpyruvate phosphomutase (PF13714; HMM-score: 13.8)
    TIM_barrel (CL0036) ThiG; Thiazole biosynthesis protein ThiG (PF05690; HMM-score: 13)
    DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 12.7)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003209
    • TAT(Tat/SPI): 0.000081
    • LIPO(Sec/SPII): 0.000378
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGADELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQQVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQTDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulators: CodY* (repression) regulon, HisR* (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise  
    HisR(TF)important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise  

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Anne Berscheid, Peter Sass, Konstantin Weber-Lassalle, Ambrose L Cheung, Gabriele Bierbaum
    Revisiting the genomes of the Staphylococcus aureus strains NCTC 8325 and RN4220.
    Int J Med Microbiol: 2012, 302(2);84-7
    [PubMed:22417616] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]