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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_03011
  • pan locus tag?: SAUPAN006431000
  • symbol: hisB
  • pan gene symbol?: hisB
  • synonym:
  • product: imidazoleglycerol-phosphate dehydratase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_03011
  • symbol: hisB
  • product: imidazoleglycerol-phosphate dehydratase
  • replicon: chromosome
  • strand: -
  • coordinates: 2784828..2785406
  • length: 579
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGATTTATCAAAAACAACGAAACACAGCTGAAACGCAACTAAATATTTCAATATCTGAT
    GATCAGTCACCATCGCATATTAATACAGGTGTGGGCTTTTTAAATCATATGTTAACCTTG
    TTTACATTTCATAGCGGTCTGTCATTAAACATTGAGGCACAAGGTGATATTGACGTAGAT
    GATCACCACGTAACTGAAGATATCGGCATTGTCATTGGCCAATTGTTACTTGAAATGATT
    AAAGATAAAAAGCATTTCGTTCGTTATGGAACGATGTACATTCCAATGGATGAAACATTA
    GCACGTGTCGTTGTGGATATAAGTGGGCGCCCATACCTATCATTCAATGCATCATTAAGT
    AAAGAAAAAGTTGGTACGTTTGATACGGAGTTAGTAGAAGAATTTTTTAGAGCGGTCGTA
    ATCAATGCAAGATTAACAACGCATATTGATTTAATTCGTGGAGGTAATACACACCATGAA
    ATTGAAGCTATATTCAAAGCGTTTTCCCGTGCATTAGGCATAGCGCTAACTGCAACTGAT
    GATCAGCGTGTGCCGTCATCGAAAGGTGTGATTGAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    579

⊟Protein[edit | edit source]

Protein Data Bank: 2AE8

⊟General[edit | edit source]

  • locus tag: SAOUHSC_03011
  • symbol: HisB
  • description: imidazoleglycerol-phosphate dehydratase
  • length: 192
  • theoretical pI: 5.4988
  • theoretical MW: 21456.2
  • GRAVY: -0.0807292

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.19?  ExPASy
    Imidazoleglycerol-phosphate dehydratase D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O
  • TIGRFAM:
  • TheSEED  :
    • Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19)
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19)
    and 1 more
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19)
  • PFAM:
    S5 (CL0329) IGPD; Imidazoleglycerol-phosphate dehydratase (PF00475; HMM-score: 200.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.032907
    • TAT(Tat/SPI): 0.008802
    • LIPO(Sec/SPII): 0.000557
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFHSGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLARVVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHEIEAIFKAFSRALGIALTATDDQRVPSSKGVIE

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CodY* (repression) regulon, HisR* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    HisR*(TF)important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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